Results 181 to 190 of about 67,993 (297)

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Protein hydrolysates in cell culture: Toward multi‐omics characterization

Biotechnology Progress, EarlyView.
While protein hydrolysates are widely used in cell culture applications, they remain undefined and variable products. Multi‐omic characterization evaluating composition and function can transition hydrolysates toward semi‐defined media components.
Michelle Combe, Brandon M. Wrage, Angel Varela‐Rohena, Stanislav Sokolenko   +3 more
wiley   +1 more source

Gauging lipid vulnerabilities in mRNA-LNPs under various environmental stressors through TIMS-TOF analysis. [PDF]

RSC Adv
Girgis M, Saed A, Alishetty S, Carrasco M, O'neill D, Baby N, Petruncio G, Hoemann C, Albano M, Peng X, Wang B, Forsberg E, Rahil MN, Saleh A, Paige M.   +14 more
europepmc   +1 more source

β‐1,4‐galactosyltransferase 1 inhibitors modulate cellular N‐glycan profiles

Biotechnology Progress, EarlyView.
Graphical representation of the study workflow used to investigate the effects of known in vitro galactosyltransferase inhibitors on cell culture performance and recombinant IgG galactosylation. Abstract N‐glycosylation is a key post‐translational modification of proteins influencing their physicochemical properties as well as biological activity. This
Jaka Kranjc, Stane Pajk, Marko Anderluh, Anja Pišlar, Matjaž Brinc   +4 more
wiley   +1 more source

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo‐Voigt hard modeling during anion‐exchange chromatography

Biotechnology Progress, EarlyView.
Abstract In biopharmaceutical manufacturing, protein aggregation is a critical quality attribute, necessitating rapid and reliable analytical strategies during downstream processes like anion‐exchange chromatography (AEX). While Raman spectroscopy enables continuous monitoring of protein secondary structure, standard data‐driven regression models ...
Jakob Heyer‐Müller, Robin Schiemer, Lars Robbel, Michael Schmitt, Jürgen Hubbuch   +4 more
wiley   +1 more source

Toxicity Profile of the Oceanic Pufferfish <i>Lagocephalus lagocephalus</i> in the Eastern Atlantic Area. [PDF]

Mar Drugs
Nocchi N, Santana-Mayor Á, Conde-Díaz A, Hernández-Lopez V, Rodríguez Hernández A, Brito A, Díaz-Marrero AR, Fernández JJ.   +7 more
europepmc   +1 more source

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP)

Biotechnology Progress, EarlyView.
Abstract High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical ...
Aylin Mohammadzadehmarandi, Nate Heller, Mohammadreza Ahmadimashhadi, Jon Petrone, Nicholas Marchand, Siavash Darvishmanesh, Ka Zhang, Stefano Menegatti, Andrew L. Zydney   +8 more
wiley   +1 more source

Dispersive micro-solid-phase extraction coupled to UHPLC-Q-TOF-MS and UHPLC-QqQ-MS for suspect and target screening of illicit drugs and pharmaceuticals in wastewater. [PDF]

Anal Bioanal Chem
Nascimento MM, Mesquita PRR, Cunha RL, da Silva Junior ALS, da Rocha GO, de Jesus RM, de P Pereira PA, de Andrade JB.   +7 more
europepmc   +1 more source

Late‐Stage Modification of Halotryptophan‐Containing Peptides Via Negishi Cross‐Coupling

Chemistry – A European Journal, EarlyView.
Negishi cross‐coupling provides access to the late‐stage functionalization of bromotryptophan‐containing peptides under comparatively mild conditions and is applicable to a range of peptide substrates in the investigated model systems. The examined alkyl iodides and variation of N‐terminal residues demonstrate the feasibility of the transformation in a
Laura Cerveson, Marcel‐Jannik Heldt, Norbert Sewald   +2 more
wiley   +1 more source

Correction: Biological activity, UHPLC-MS phytochemical profiling, and computational studies of the leaf extract of Acridocarpus socotranus. [PDF]

Sci Rep
Al-Madhagi WM, Ali NAA, Al-Fatimi MA, Anwar S, Ahemad N, Mansi IA, Mhaidat NM, Al-Badani RN, Almarabi I, Naouar A, Al-Balas Q, Al-Khulaidi A.   +11 more
europepmc   +1 more source