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2023
This chapter considers ultracentrifugation, which provides methods for the characterisation of the sizes and shapes of macromolecules and macromolecular assemblies. Ultracentrifugation is used in three ways: velocity sedimentation, equilibrium sedimentation, and density-gradient sedimentation.
Peter Atkins +3 more
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This chapter considers ultracentrifugation, which provides methods for the characterisation of the sizes and shapes of macromolecules and macromolecular assemblies. Ultracentrifugation is used in three ways: velocity sedimentation, equilibrium sedimentation, and density-gradient sedimentation.
Peter Atkins +3 more
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Calibrating analytical ultracentrifuges
European Biophysics Journal, 2021Analytical ultracentrifugation (AUC) is based on the concept of recording and analyzing macroscopic macromolecular redistribution that results from a centrifugal force acting on the mass of suspended macromolecules in solution. Since AUC rests on first principles, it can provide an absolute measurement of macromolecular mass, sedimentation and ...
Huaying Zhao +3 more
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Analytical Ultracentrifugation
Current Protocols in Protein Science, 1996AbstractAnalytical ultracentrifugation is one of the most powerful, though as yet underexploited, techniques available to molecular biology and biochemistry. This overview describes applications for analytical ultracentrifugation along with important considerations relating to experimental design.
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Lipid Exchange by Ultracentrifugation
2016Lipids play an important role in maintaining P-type ATPase structure and function, and often they are crucial for ATPase activity. When the P-type ATPases are in the membrane, they are surrounded by a mix of different lipid species with varying aliphatic chain lengths and saturation, and the complex interplay between the lipids and the P-type ATPases ...
Drachmann, Nikolaj Düring +1 more
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Analytical Ultracentrifugation
2005Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over ...
Z Aziz +42 more
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