Results 141 to 150 of about 100,244 (261)

Protocol to isolate endosomal and small extracellular vesicles from cultured cells through ultracentrifugation. [PDF]

STAR Protoc
Domingues MN, Palhinhas L, Pereira P, Ferreira JV.   +3 more
europepmc   +1 more source

Morphological Transitions in the Self‐Assembly of Rigid Sphere–Rod Amphiphiles Achieved by Adjusting the Linker Length

Chemistry – A European Journal, EarlyView.
Linker length regulates the self‐assembly of rigid sphere–rod amphiphiles. Long linkers enable rigid sphere–rod molecules to undergo solvent‐dependent conformational switching between I‐ and T‐shaped states, driving morphological transitions of the assemblies from striped spherical structures to onion‐like structures.
Jingfan Wei, Yifan Zhou, Kexing Xiao, Xiangqian Li, Hao Liu, Tianbo Liu   +5 more
wiley   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Reply: Extracellular vesicle-mediated signalling in human rescue <i>in vitro</i> maturation: methodological and mechanistic considerations. [PDF]

Hum Reprod Open
Makieva S, Saenz-de-Juano MD, Almiñana C, Bauersachs S, Bernal-Ulloa S, Xie M, Velasco AG, Cervantes N, Sachs M, Ulbrich SE, Leeners B.   +10 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Comparison of methods for isolation of extracellular vesicles from bronchoalveolar lavage fluid. [PDF]

Extracell Vesicles Circ Nucl Acids
Fraser ME, Patel R, Shinabery T, Zagorski J, Chen L, Dhillon NK, Clauss M, Doud EH, Mosley AL, Gaston B, Twigg Iii HL.   +10 more
europepmc   +1 more source

Quadruplex Bioactive FAND for Treating Acute Liver Failure Induced by Acetaminophen or Hepatectomy

Exploration, EarlyView.
This work developed a bioactive quadruplex FANDHP@FuEVs with intrinsic inflammation‐targeting properties of M2‐EVs and MSC‐EVs, harnessing the synergistic biological activities by leveraging four natural active components—M2‐EVs, MSC‐EVs, HGF, and PPC—to deliver pleiotropic therapies, including antioxidant, anti‐inflammatory, pro‐regenerative, and M1 ...
Meng Sun, Fang Fang, Juan Liu, Yueyun Fan, Sa Wang, Chuang Zhang, Weiyu Li, Zhengyang Quan, Dongxu Zhao, Min Hu, Jinfeng Zhang   +10 more
wiley   +1 more source

Mesenchymal stem cell-derived extracellular vesicles for osteochondral defect regeneration: a systematic review and meta-analysis of preclinical studies. [PDF]

J Transl Med
Wen Y, Meng G, Yang S, Mei H.
europepmc   +1 more source

Comparative analysis of different biophysical techniques for exosome characterization. [PDF]

Sci Rep
Yu X, Wang Z, Zhang R, Guo W, Chang Q, Chu W, Zeng C, Wang L, Yu C, Li W.   +9 more
europepmc   +1 more source

Unraveling the Plasma Extracellular Vesicles Proteome: Predictors of Resistance in Immunotherapy of Advanced Melanoma

International Journal of Cancer, EarlyView.
ABSTRACT Despite substantial clinical benefit from immune checkpoint inhibitors (ICI), advanced melanoma remains challenging due to frequent treatment resistance. Resistance may be intrinsic (primary) or emerge over time (secondary). Biomarkers predicting distinct resistance phenotypes before therapy are lacking.
Julian Kött, Glenn Geidel, Carmen M. T. Roeper, Lucija Ačkar, Bente Siebels, Hannah Voß, Hartmut Schlüter, Michaela Schweizer, Kilian Müller, Alessandra Rünger, Tim Zell, Benjamin Deitert, Ali Zeinal‐Abedini, Isabel Heidrich, Stefan W. Schneider, Klaus Pantel, Daniel J. Smit, Christoffer Gebhardt   +17 more
wiley   +1 more source