Results 61 to 70 of about 166,094 (382)

Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales

Journal of Extracellular Vesicles, 2018
Extracellular vesicles (EVs) provide a complex means of intercellular signalling between cells at local and distant sites, both within and between different organs.
Anna-Kristin Ludwig, Kyra de Miroschedji, T. Doeppner, V. Börger, Johannes Ruesing, V. Rebmann, S. Durst, S. Jansen, Michel Bremer, E. Behrmann, B. Singer, H. Jastrow, J. Kuhlmann, F. El Magraoui, H. Meyer, D. Hermann, B. Opalka, S. Raunser, M. Epple, P. Horn, B. Giebel   +20 more
semanticscholar   +1 more source

Thermal proteome profiling and proteome analysis using high‐definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next‐generation galeterone analog VNPP433‐3β in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Elevated level of cholesterol is positively correlated to prostate cancer development and disease severity. Cholesterol‐lowering drugs, such as statins, are demonstrated to inhibit prostate cancer. VNPP433‐3β interrupts multiple signaling and metabolic pathways, including cholesterol biosynthesis, AR‐mediated transcription of several oncogenes, mRNA 5′
Retheesh S. Thankan, Elizabeth Thomas, Mehari M. Weldemariam, Puranik Purushottamachar, Weiliang Huang, Maureen A. Kane, Yuji Zhang, Nicholas Ambulos, Bi‐Dar Wang, David Weber, Vincent C. O. Njar   +10 more
wiley   +1 more source

Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality

Journal of Extracellular Vesicles, 2019
Extracellular vesicles (EVs) from a variety of stem cell sources are believed to harbour regenerative capacity, which may be exploited for therapeutic purposes.
Alice Gualerzi, Sander Alexander Antonius Kooijmans, Stefania Niada, Silvia Picciolini, Anna Teresa Brini, Giovanni Camussi, Marzia Bedoni   +6 more
doaj   +1 more source

Characterization of Plasma-Derived Extracellular Vesicles Isolated by Different Methods: A Comparison Study

Bioengineering, 2019
Extracellular vesicles (EV) are small membrane structures released by cells that act as potent mediators of intercellular communication. The study of EV biology is important, not only to strengthen our knowledge of their physiological roles, but also to ...
Esther Serrano-Pertierra, Myriam Oliveira-Rodríguez, Montserrat Rivas, Pedro Oliva, Javier Villafani, Ana Navarro, M. Carmen Blanco-López, Eva Cernuda-Morollón   +7 more
doaj   +1 more source

CNS Mitochondria‐Derived Vesicle in Blood: Potential Biomarkers for Brain Mitochondria Dysfunction

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial dysfunction is a hallmark of neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD). Our goal was to develop practical, noninvasive methods to assess mitochondrial status through the detection of mitochondria‐derived vesicles (MDVs).
Qi Liu, Wentao Chen, Yahong Wu, Zhen Guo, Jun Chen, Chen Tian, Pan Wang, Shaopeng Zeng, Bin Xu, Jing Duan, Shilong Han, Xiao Xiong, Jing Zhang   +12 more
wiley   +1 more source

Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing—A Comparative Study

Sensors, 2020
Exosomes are cell-derived nanovesicles released into biological fluids, which are involved in cell-to-cell communication. The analysis of the content and the surface of the exosomes allow conclusions about the cells they are originating from and the ...
Silio Lima Moura, Mercè Martì, María Isabel Pividori   +2 more
doaj   +1 more source

Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content

Frontiers in Microbiology, 2021
Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are mediators of cell survival and pathogenesis by facilitating virulence factor dissemination and resistance to antimicrobials.
Shelby L. Reimer, Daniel R. Beniac, Shannon L. Hiebert, Timothy F. Booth, Patrick M. Chong, Garrett R. Westmacott, George G. Zhanel, Denice C. Bay   +7 more
doaj   +1 more source

Crystallization of Adenylylsulfate Reductase from Desulfovibrio gigas: A Strategy Based on Controlled Protein Oligomerization [PDF]

, 2011
Adenylylsulfate reductase (adenosine 5′-phosphosulfate reductase, APS reductase or APSR, E.C.1.8.99.2) catalyzes the conversion of APS to sulfite in dissimilatory sulfate reduction. APSR was isolated and purified directly from massive anaerobically grown
Chan, Sunney I., Chen, Chun-Jung, Chiang, Yuan-Lan, Chuankhayan, Phimonphan, Fang, Jou-Yin, Hsieh, Yin-Cheng, Huang, Yen-Chieh, Liu, Ming-Yih, Wang, Vincent C.-C., Yang, Ming-Chi   +9 more
core   +1 more source

Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

PLoS ONE, 2015
Background Exosomes are emerging targets for biomedical research. However, suitable methods for the isolation of blood plasma-derived exosomes without impurities have not yet been described.
T. Baranyai, Kata Herczeg, Zsófia Onódi, I. Voszka, K. Módos, N. Marton, G. Nagy, Imre Mäger, M. Wood, S. El Andaloussi, Z. Pálinkás, Vikas Kumar, P. Nagy, Á. Kittel, E. Buzás, P. Ferdinandy, Z. Giricz   +16 more
semanticscholar   +1 more source

The Potential for Extracellular Vesicles in Nanomedicine: A Review of Recent Advancements and Challenges Ahead

Advanced Biology, EarlyView.
Extracellular vesicles (EVs) play a dual role in diagnostics and therapeutics, offering innovative solutions for treating cancer, cardiovascular, neurodegenerative, and orthopedic diseases. This review highlights EVs’ potential to revolutionize personalized medicine through specific applications in disease detection and treatment.
Farbod Ebrahimi, Anjali Kumari, Samaneh Ghadami, Saqer Al Abdullah, Kristen Dellinger   +4 more
wiley   +1 more source