The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation [PDF]
We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or
A Maas +46 more
core +13 more sources
Erythrocytes as Carriers of Therapeutic Enzymes. [PDF]
Therapeutic enzymes are administered for the treatment of a wide variety of diseases. They exert their effects through binding with a high affinity and specificity to disease-causing substrates to catalyze their conversion to a non-noxious product, to ...
Bax, BE
core +1 more source
Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood [PDF]
The activity of urease purified from Proteus mirabilis bacteria wasestimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained asstandard in size (20- 40)nm, purity 99%. The enzyme activity was estimated byincubating the pure enzyme with
Hadeel Dawud, Entesar Ali
doaj +1 more source
Managemental influences on the selective proliferation of two strains of haemolytic Escherichia coli in weaned pigs [PDF]
In an experimental study on a piggery it was found that haemolytic Escherichia coli of O-serotypes 138 or 139 proliferated in the intestinal tracts of pigs following weaning, with E.
Hampson, D.J. +3 more
core +2 more sources
RovM and CsrA Negatively Regulate Urease Expression in Yersinia pseudotuberculosis
Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium Yersinia ...
Qingyun Dai +10 more
doaj +1 more source
In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatography-based metabolomic analysis, urea needed to be degraded by urease.
Jungyeon Kim +5 more
doaj +1 more source
Temperature and humidity (TH) post‐treatment is a relatively mild way to change the silk fibroin (SF) secondary structure. Here, urease is immobilized in SF scaffold (SFS) by TH post‐treatment, and the effects of annealing on the immobilized urease ...
Xiao‐Qin Qian +5 more
doaj +1 more source
Prevalence of Helicobacter pylori in patients with gastro-oesophageal reflux disease : systematic review. [PDF]
Objectives: To ascertain the prevalence of Helicobacter pylori in patients with gastro-oesophageal reflux disease and its association with the disease.
Childs, S. +3 more
core +2 more sources
The Maturation Pathway of Nickel Urease
Maturation of urease involves post-translational insertion of nickel ions to form an active site with a carbamylated lysine ligand and is assisted by urease accessory proteins UreD, UreE, UreF and UreG.
Yap Shing Nim, Kam-Bo Wong
doaj +1 more source
Soil enzyme activities in the rhizosphere of field-grown sugar beet inoculated with the biocontrol agent Pseudomonas fluorescens F113 [PDF]
The original publication is available at www.springerlink.com . Copyright Springer DOI : 10.1007/s003740050397Pseudomonas fluorescens F113, which produces the antimicrobial compound 2,4-diacetylphloroglucinol is a prospective biocontrol agent.
Lynch, J.M. +4 more
core +1 more source

