Functional interplay between NTP leaving group and base pair recognition during RNA polymerase II nucleotide incorporation revealed by methylene substitution. [PDF]
, 2016 RNA polymerase II (pol II) utilizes a complex interaction network to select and incorporate correct nucleoside triphosphate (NTP) substrates with high efficiency and fidelity.
Chong, Jenny +10 more
core +2 more sources
Light‐Triggered CRISPR/Cas12a for Genomic Editing and Tumor Regression
Angewandte Chemie, EarlyView.A photo‐triggered CRISPR/Cas12a machinery for gene editing is introduced. The machinery is applied for endogenous DNA methyltransferase 1 (DNMT1) gene editing and for in vitro disruption of the hepatocyte growth factor (HGF) gene. The machinery is used for in vivo knockout and disruption of the HGF gene in HepG2 tumors, resulting in effective apoptosis
Hong Liu +7 more
wiley +2 more sources
Transition behavior of the seizure dynamics modulated by the astrocyte inositol triphosphate noise [PDF]
, 2021 Epilepsy is a neurological disorder with recurrent seizures of complexity and randomness. Until now, the mechanism of epileptic randomness has not been fully elucidated. Inspired by the recent finding that astrocyte GTPase-activating protein (G-protein)-coupled receptors could be involved in stochastic epileptic seizures, we proposed a neuron-astrocyte
arxiv +1 more source
A Comparative Study of the Structural Dynamics of Four Terminal Uridylyl Transferases. [PDF]
, 2017 African trypanosomiasis occurs in 36 countries in sub-Saharan Africa with 10,000 reported cases annually. No definitive remedy is currently available and if left untreated, the disease becomes fatal.
Amaro, Rommie E +2 more
core +1 more source
Lethal Mutagenesis of Picornaviruses with N-6-Modified Purine Nucleoside Analogues [PDF]
, 2008 RNA viruses exhibit extraordinarily high mutation rates during genome replication. Nonnatural ribonucleosides that can increase the mutation rate of RNA viruses by acting as ambiguous substrates during replication have been explored as antiviral agents ...
Barr, Eric W. +11 more
core +4 more sources
Unzipping and binding of small interfering RNA with single walled Carbon Nanotube: a platform for small interfering RNA delivery [PDF]
J. Chem. Phys. 136, 065106 (2012), 2012 In an effort to design efficient platform for siRNA delivery, we combine all atom classical and quantum simulations to study the binding of small interfering RNA (siRNA) by pristine single wall carbon nanotube (SWCNT). Our results show that siRNA strongly binds to SWCNT surface via unzipping its base-pairs and the propensity of unzipping increases with
arxiv +1 more source
Chemistry Central Journal, 2008 Background Although the necessity of divalent magnesium and manganese for full activity of sugar nucleotidyltransferases and glycosyltransferases is well known, the role of these metal cations in binding the substrates (uridine 5'-triphosphate, glucose-1-
Pohl Nicola L +2 more
doaj +1 more source
Human Deoxycytidine Kinase Is a Valuable Biocatalyst for the Synthesis of Nucleotide Analogues [PDF]
, 2019 Natural ribonucleoside-5’-monophosphates are building blocks for nucleic acids which are used for a number of purposes, including food additives. Their analogues, additionally, are used in pharmaceutical applications.
Hellendahl, Katja F. +4 more
core +1 more source
Lethal Mutagenesis of Poliovirus Mediated by a Mutagenic Pyrimidine Analogue [PDF]
, 2007 Lethal mutagenesis is the mechanism of action of ribavirin against poliovirus (PV) and numerous other RNA viruses. However, there is still considerable debate regarding the mechanism of action of ribavirin against a variety of RNA viruses.
Brown, Daniel M. +11 more
core +4 more sources
Model for the unidirectional motion of a dynein molecule [PDF]
Phys. Rev. E 77, 051916 (2008), 2008 Cytoplasmic dyneins transport cellular organelles by moving on a microtubule filament. It has been found recently that depending on the applied force and the concentration of the adenosine triphosphate (ATP) molecules, dynein's step size varies. Based on these studies, we propose a simple model for dynein's unidirectional motion taking into account the
arxiv +1 more source