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Two-step hydrolysis method for monosaccharide composition analysis of natural polysaccharides rich in uronic acids

, 2020
Monosaccharide composition analysis is important to characterize the structure of complex carbohydrates. However, techniques for analyzing this in acidic polysaccharides are immature, due to incomplete hydrolysis.
Hui-Fang Shi   +6 more
semanticscholar   +1 more source

On the determination of galactosamine-uronic acid

Analytical Biochemistry, 1966
Abstract A procedure for measuring galactosamine-uronic acid was developed by modifying the Levvy-McAllan (10) colorimetric procedures for free hexosamines. The modification developed included a lower tetraborate concentration and a longer alkaline heating period. A shorter N -acetylation period is required than used by Roseman and Daffner (9). With
S T, Brownlee, R W, Wheat
openaire   +2 more sources

Identification of uronic acids in mucopolysaccharides

Archives of Biochemistry and Biophysics, 1963
The isolation or identification of uronic acids from acid mucopolysaccharides can be achieved by hydrolysis with formic acid and chromatography of the hydrolyzates.
B, RADHAKRISHNAMURTHY, G S, BERENSON
openaire   +2 more sources

Synthesis and Application of Uronic Acids

Current Organic Chemistry, 2014
This review concerns selected methods for the synthesis of uronic acids and uronic acid-containing oligosaccharides. Construction of uronic acids is broadly represented by methods based on carbohydrates and non-carbohydrates as well as de novo synthesis.
Nowogródzki, Marcin, Młynarski, Jacek
openaire   +2 more sources

Automated ion-exchange chromatography of uronic acids and uronic acid containing oligosaccharides

Analytical Biochemistry, 1968
Abstract An automated anion-exchange chromatographic procedure for the separation of uronic acids and uronic acid containing oligosaccharides is described. The method has been applied, in particular, to the separation and quantitation of l -iduronic and d -glucuronic acid in hydrolyzates of dermatan sulfate, and the hydrolysis conditions required ...
L A, Fransson, L, Rodén, M L, Spach
openaire   +2 more sources

Uronic acid determination

Talanta, 1958
Abstract Modifications of the McCready, Swensen and Maclay apparatus for uronic acid determination are described which enable determinations to be made on 20-mg samples.
S.A. Barker   +3 more
openaire   +1 more source

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