We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Efeitos da radiação ultravioleta-A e ultravioleta-B sobre os embriões do camarão de água-doce Macrobrachium olfersi (Crustacea, Decapoda) e o papel da radiação ultravioleta-A na fotorreativação [PDF]
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Biologia Celular e do Desenvolvimento, Florianópolis, 2011Nas últimas décadas os pesquisadores têm voltado sua atenção para os ...
Cardoso, Valquíria Machado
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Фотоморфогенетична відповідь рослин гороху (Pisum sativum L.) на дію ультрафіолетової радіації
Aim. The comparation of photomorphogenetic response of pea plants in wide dose range of UV-C and UV-B irradiation was studied. Methods. Pea plants (Pisum sativum L.) cultivar Aronis were grown in water culture during 10 days and then were irradiated by ...
V. V. Zhuk +2 more
doaj +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Influência de fatores exógenos na germinação de esporos e da radiação ultravioleta B (RUV B) no desenvolvimento de gametófitos de Acrostichum Danaeifolium Langsd. & Fisch (Polypodiopsida, Pteridaceae): uma pteridófita do manguezal [PDF]
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Biologia VegetalA distribuição da vegetação mundial vem se alterando ao longo do tempo, mas atualmente as alterações climáticas ...
Freitas, Michelle Carneiro de Andrade de
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UV-B induced morphological changes - an enigma [PDF]
Peer ...
Klem, Karel +3 more
core +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Effects of salicylic acid pre-treatment on cadmium and/or UV-B stress in soybean seedlings
The present study examined the effect of salicylic acid (SA) pre-treatment on soybean seedlings exposed to cadmium and/or UV-B stress. Dry mass, pigment content, net photosynthetic rate (PN), stomatal conductance (gs), and transpiration rate (E) were ...
Y. Y. Li +4 more
core +1 more source
Regulation of nucleotide excision repair by UV-DDB: prioritization of damage recognition to internucleosomal DNA [PDF]
How tightly packed chromatin is thoroughly inspected for DNA damage is one of the fundamental unanswered questions in biology. In particular, the effective excision of carcinogenic lesions caused by the ultraviolet (UV) radiation of sunlight depends on ...
Naegeli, H +29 more
core +2 more sources

