Results 71 to 80 of about 10,674,979 (295)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Solar ultraviolet irradiance in the ozone hole region in Antarctica
Antarctic Record, 2001 To analyze characteristics of solar ultraviolet irradiance in the ozone hole region, solar ultraviolet-B (UV-B : 280-315nm) and ultraviolet-A (UV-A : 315-400nm) irradiance at noon were measured from October 4,1999 to January 31,2000 at Syowa Station in ...
Shu Takeshita +3 more
doaj +1 more source
BMC Plant Biology, 2010 Background Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV ...
Martinez-Zapater Jose M +5 more
doaj +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Journal of Plant Interactions, 2018 An enhanced UV-B radiation (5.0 kJ m−2) was supplied before, during, and after Magnaporthe oryzae infection. The effects of single and compound stress of the UV-B radiation and M.
Yongmei He +6 more
doaj +1 more source
Efeitos da radiação ultravioleta-A e ultravioleta-B sobre os embriões do camarão de água-doce Macrobrachium olfersi (Crustacea, Decapoda) e o papel da radiação ultravioleta-A na fotorreativação [PDF]
, 2011 Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Biologia Celular e do Desenvolvimento, Florianópolis, 2011Nas últimas décadas os pesquisadores têm voltado sua atenção para os ...
Cardoso, Valquíria Machado
core
Regulation of nucleotide excision repair by UV-DDB: prioritization of damage recognition to internucleosomal DNA [PDF]
, 2011 How tightly packed chromatin is thoroughly inspected for DNA damage is one of the fundamental unanswered questions in biology. In particular, the effective excision of carcinogenic lesions caused by the ultraviolet (UV) radiation of sunlight depends on ...
Naegeli, H +23 more
core +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Frontiers in Plant Science, 2016 The depletion of the ozone layer in the stratosphere has led to a dramatic spike in ultraviolet B (UV-B) intensity and increased UV-B light levels.
Min Young Yoon +10 more
doaj +1 more source