Regulatory assembly of the vacuolar proton pump VOV1-ATPase in yeast cells by FLIM-FRET
We investigate the reversible disassembly of VOV1-ATPase in life yeast cells by time resolved confocal FRET imaging. VOV1-ATPase in the vacuolar membrane pumps protons from the cytosol into the vacuole. VOV1-ATPase is a rotary biological nanomotor driven
Batisse, Claire +4 more
core +1 more source
Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae [PDF]
In the budding yeast Saccharomyces cerevisiae, the Cdc42 effector Ste20 plays a crucial role in the regulation of filamentous growth, a response to nutrient limitation.
Höfken, T, Kane, PM, Li, SC, Lin, M
core +1 more source
Molecular Cloning and Expression of Three Isoforms of the 100-kDa a Subunit of the Mouse Vacuolar Proton-translocating ATPase [PDF]
We have identified cDNAs encoding three isoforms (a1, a2, and a3) of the 100-kDa a subunit of the mouse vacuolar proton-translocating ATPase (V-ATPase). The predicted protein sequences of the three isoforms are 838, 856, and 834 amino acids, respectively, and they display approximately 50% identity between isoforms.
T, Nishi, M, Forgac
openaire +2 more sources
Dynamic subcellular translocation of V-type H+ -ATPase is essential for biomineralization of the diatom silica cell wall. [PDF]
Diatom cell walls, called frustules, are main sources of biogenic silica in the ocean and their intricate morphology is an inspiration for nanoengineering.
Hildebrand, Mark +2 more
core
Vacuolar H+-Pyrophosphatase AVP1 is Involved in Amine Fungicide Tolerance in Arabidopsis thaliana and Provides Tridemorph Resistance in Yeast [PDF]
Amine fungicides are widely used as crop protectants. Their success is believed to be related to their ability to inhibit postlanosterol sterol biosynthesis in fungi, in particular sterol-Δ8,Δ7-isomerases and sterol-Δ14-reductases, with a concomitant ...
Agustín eHernández +9 more
core +1 more source
RNA interference (RNAi) technologies, host‐induced gene silencing (HIGS) and spray‐induced gene silencing (SIGS), potentially offer sustainable crop protection. However, efficacy, costs, regulatory clarity, and socio‐environmental impacts require further evaluation for broader use.
Elisabetta Sergi +12 more
wiley +1 more source
Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes [PDF]
The vacuolar-type H+-translocating ATPase (v-H+-ATPase) has been implicated in the amino aciddependent activation of the mechanistic target of rapamycin complex 1 (MTORC1), an important regulator of macroautophagy. To reveal the mechanistic links between
Damme, Markus +11 more
core +2 more sources
Proteostasis of organelles in aging and disease
Cells rely on regulated proteostasis mechanisms to keep their internal compartments functioning properly. When these mechanisms fail, damaged proteins accumulate, disrupting organelles, such as the nucleus, mitochondria, endoplasmic reticulum, Golgi, and lysosomes, as well as membraneless organelles, such as stress granules, processing bodies, the ...
Yara Nabawi +5 more
wiley +1 more source
Rotating with the brakes on and other unresolved features of the vacuolar ATPase [PDF]
The rotary ATPase family is comprised of the ATP synthase (F-ATPase), vacuolar ATPase (V-ATPase) and acrahael ATPase (A-ATPase). These either predominantly utilise a proton gradient for ATP synthesis or use ATP to produce a proton gradient, driving ...
Abrahams +45 more
core +1 more source
This review examines salt glands in exo‐recretohalophytes, in which epidermal stem cells differentiate into unicellular, bicellular, or multicellular salt glands. Salt ions are transported to the leaves via the transpiration stream and enter salt glands through symplastic and apoplastic pathways. Finally, salt glands actively secrete salt ions from the
Limin Wang +11 more
wiley +1 more source

