Results 41 to 50 of about 2,566,277 (265)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Increased efficacy for in-house validation of real-time PCR GMO detection methods [PDF]

, 2009
To improve the efficacy of the in-house validation of GMO detection methods (DNA isolation and real-time PCR, polymerase chain reaction), a study was performed to gain insight in the contribution of the different steps of the GMO detection method to the ...
I. M. J. Scholtens, L. Hougs, B. Molenaar, Kok, E.J., Scholtens-Toma, I.M.J., Molenaar, B., Thissen, J.T.N.M., E. J. Kok, Voet, H., van der, Hougs, L., J. T. N. M. Thissen, H. van der Voet, van der Voet, H.   +12 more
core   +1 more source

A Comparison of State-Based Modelling Tools for Model Validation

, 2008
In model-based testing, one of the biggest decisions taken before modelling is the modelling language and the model analysis tool to be used to model the system under investigation. UML, Alloy and Z are examples of popular state-based modelling languages.
Aydal, Emine, Mark Utting, Woodcock, Jim, Emine G. Aydal, Aydal, Emine G., Utting, Mark, Aydal, Emine G, Jim Woodcock   +7 more
core   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Portuguese validation of the Internet addiction test: an empirical study [PDF]

, 2014
Background and aims: Research into Internet addiction (IA) has increased greatly over the last decade. Despite its various definitions and general lack of consensus regarding its conceptualisation amongst researchers, instruments for measuring this ...
Griffiths, M, Pontes, H, Ivone M. Patrão, Patrão, I.M., Patrão, Ivone Alexandra Martins, Griffiths, M.D., Pontes, Halley de Oliveira Miguel, Pontes, Halley M., Griffiths, Mark D., Mark D. Griffiths, Pontes, Halley, Patrão, I, Halley M. Pontes, Patrão, Ivone M.   +13 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Development and validation of a paediatric long-bone fracture classification : a prospective multicentre study in 13 European paediatric trauma centres [PDF]

, 2011
Background: The aim of this study was to develop a child-specific classification system for long bone fractures and to examine its reliability and validity on the basis of a prospective multicentre study.
Marzi, Ingo, Ralf Kraus, Lutz von Laer, Kraus, Ralf, Kaiser, Martin, Röder Christoph, von Laer Lutz, Schneidmüller, Dorien, Martin Kaiser, Dietrich, Daniel, Christoph Röder, Dietrich, Daniel J., Ingo Marzi, Kaiser Martin, Marzi Ingo, Röder, Christoph, Schneidmüller Dorien, Dietrich Daniel, von Laer, Lutz, Laer, Lutz von, Kraus Ralf, Dorien Schneidmüller, Daniel Dietrich   +22 more
core   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source