Results 151 to 160 of about 57,276 (313)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Multiple solutions for possibly degenerate equations in divergence form

Electronic Journal of Differential Equations, 2016
Via variational methods, we establish the existence of at least two distinct weak solutions for the Dirichlet problem associated to a possibly degenerate equation in divergence form.
Andrea Pinamonti
doaj  

Approximations Of Posterior Distributions In Blind Deconvolution

, 2008
In this paper the blind deconvolution problem is formulated using the variational framework. With its use approximations of the involved probability distributions are developed resulting in two algorithms for the estimation of the posterior distributions
Rafael Molina, Using Variational Methods, Javier Mateos   +2 more
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Nontrivial solutions for noncooperative elliptic systems at resonance

Electronic Journal of Differential Equations, 2001
In this article we establish the existence of a nonzero solution for variational noncooperative elliptic systems under Dirichlet boundary conditions and a resonant condition at infinity.
Elves A. B. Silva
doaj  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Existence and nonexistence of nontrivial solutions for Choquard type equations

Electronic Journal of Differential Equations, 2016
In this article, we consider the nonlocal problem $$ -\Delta u+u=q(x)\Big(\int_{\mathbb{R}^N}\frac{q(y)|u(y)|^p}{|x-y|^{N-\alpha}}dy \Big)|u|^{p-2}u,\quad x\in \mathbb{R}^N, $$ where $N\geq 3$, $\alpha\in (0,N)$, $\frac{N+\alpha}{N}
Tao Wang
doaj  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Variational Methods and Critical Point Theory 2013

Abstract and Applied Analysis, 2013
M. Victoria Otero-Espinar, Juan J. Nieto, Donal O’Regan, Kanishka Perera   +3 more
doaj   +1 more source