Results 101 to 110 of about 2,245,201 (365)

Forced Salivation As a Method to Analyze Vector Competence of Mosquitoes [PDF]

Journal of Visualized Experiments, 2018
Vector competence is defined as the potential of a mosquito species to transmit a mosquito-borne virus (mobovirus) to a vertebrate host. Viable virus particles are transmitted during a blood meal via the saliva of an infected mosquito. Forced salivation assays allow determining the vector potential on the basis of single mosquitoes, avoiding the use of
Stephanie Jansen, Mayke Leggewie, Anna Heitmann, Egbert Tannich, Jonas Schmidt-Chanasit, Renke Lühken   +5 more
openaire   +3 more sources

Characterization and Vector Competence Studies of Chikungunya Virus Lacking Repetitive Motifs in the 3′ Untranslated Region of the Genome

Viruses, 2021
Using reverse genetics, we analyzed a chikungunya virus (CHIKV) isolate of the Indian Ocean lineage lacking direct repeat (DR) elements in the 3′ untranslated region, namely DR1a and DR2a.
Yauhen Karliuk, Anja vom Hemdt, Janett Wieseler, Martin Pfeffer, Beate M. Kümmerer   +4 more
doaj   +1 more source

Effects of Blood Meal Source on Aedes albopictus Life Table Characteristics and Vector Competence for Dengue Virus [PDF]

, 2016
Presented for World Environmental Health Day, September 26, 2016 in Greenville, North Carolina.Commercially available blood can be used as an alternative to live animals to feed mosquitoes; however, the extent to which alternative blood sources affect
Harris, Jonathan W., NC DOCKS at East Carolina University, Richards, Stephanie L., Tippett, Joshua W., van Dodewaard, Caitlin A.M.   +4 more
core  

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Analysis of switching dynamics with competing support vector machines [PDF]

Proceedings of the 2002 International Joint Conference on Neural Networks. IJCNN'02 (Cat. No.02CH37290), 2003
We present a framework for the unsupervised segmentation of switching dynamics using support vector machines. Following the architecture by Pawelzik et al., where annealed competing neural networks were used to segment a nonstationary time series, in this paper, we exploit the use of support vector machines, a well-known learning technique.
Chang, Ming-Wei, Lin, Chih-Jen, Weng, R.C.   +2 more
openaire   +5 more sources

Horizontal and Vertical Transmission of Powassan Virus by the Invasive Asian Longhorned Tick, Haemaphysalis longicornis, Under Laboratory Conditions

Frontiers in Cellular and Infection Microbiology, 2022
The Asian longhorned tick, Haemaphysalis longicornis, is an ixodid tick native to East Asia that was first detected in North America outside a port of entry in 2017. This invasive species has since been detected in 17 states. As the invasive range of the
Wilson R. Raney, Erik J. Herslebs, Ingeborg M. Langohr, Madeline C. Stone, Meghan E. Hermance   +4 more
doaj   +1 more source

Expression systems for industrial Gram-positive bacteria with low guanine and cytosine content [PDF]

, 1997
Recent years have seen an increase in the development of gene expression systems for industrial Gram-positive bacteria with low guanine and cytosine content that belong to the genera Bacillus, Clostridium, Lactococcus, Lactobacillus, Staphylococcus and ...
Kleerebezem, Michiel,, Kuipers, Oscar P.,, Vos, Willem M. de,   +2 more
core   +2 more sources

Exploring Lutzomyia longipalpis sand flies vector competence to Leishmania major parasites.

Journal of Infectious Diseases, 2020
Lutzomyia longipalpis sand flies are the major natural vector of Leishmania infantum parasites, responsible for the transmission of visceral leishmaniasis in the New World.
P. Cecílio, A. A. Pires, J. Valenzuela, P. Pimenta, A. Cordeiro-da-Silva, N. Secundino, Fabiano Oliveira   +6 more
semanticscholar   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Vector competence and filariasis transmission in Mali [PDF]

, 2016
Lymphatic filariasis (LF) is a public health problem in 73 countries and is associated with marked morbidity and disability. It is unique because of its transmission by five main genera of mosquitoes, including Culex, Aedes, Anopheles, Mansonia and Ochlerotatus. In Mali, LF endemicity mapping in 2004 found all eight administrative regions to be endemic
openaire   +2 more sources