Results 41 to 50 of about 1,117,643 (337)

Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase [PDF]

open access: yes, 2007
Background The subcloning of a DNA fragment from an entry vector into a destination vector is a routinely performed task in molecular biology labs. Results We here present a novel benchtop procedure to achieve rapid recombination into any destination ...
Fromme, Tobias, Klingenspor, Martin
core   +3 more sources

Complete mitochondrial genomes of Anopheles stephensi and An. dirus and comparative evolutionary mitochondriomics of 50 mosquitoes

open access: yesScientific Reports, 2017
To better understand the phylogeny and evolution of mosquitoes, the complete mitochondrial genome (mitogenome) of Anopheles stephensi and An. dirus were sequenced and annotated, and a total of 50 mosquito mitogenomes were comparatively analyzed.
You-Jin Hao   +8 more
doaj   +1 more source

Genome-wide identification, characterization and classification of ionotropic glutamate receptor genes (iGluRs) in the malaria vector Anopheles sinensis (Diptera: Culicidae)

open access: yesParasites & Vectors, 2018
Background Ionotropic glutamate receptors (iGluRs) are conserved ligand-gated ion channel receptors, and ionotropic receptors (IRs) were revealed as a new family of iGluRs.
Ting-Ting Wang   +3 more
doaj   +1 more source

Efficacy and safety of dihydroartemisinin–piperaquine for the treatment of uncomplicated Plasmodium falciparum and Plasmodium vivax malaria in Papua and Sumatra, Indonesia

open access: yesMalaria Journal, 2022
Background Dihydroartemisinin–piperaquine (DHA–PPQ) has been adopted as first-line therapy for uncomplicated falciparum malaria in Indonesia since 2010.
Puji B. S. Asih   +13 more
doaj   +1 more source

HSP superfamily of genes in the malaria vector Anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance

open access: yesMalaria Journal, 2019
Background Heat shock proteins (HSPs) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress.
Feng-Ling Si   +5 more
doaj   +1 more source

Protection of Rabbits against Rabbit Hemorrhagic Disease Virus by Immunization with the VP60 Protein Expressed in Plants with a Potyvirus-Based Vector [PDF]

open access: yes, 1997
A new plum pox potyvirus (PPV)-based vector has been constructed for the expression of full-length individual foreign proteins. The foreign sequences are cloned between the NIb replicase and capsid protein (CP) cistrons. The heterologous protein is split
Fernández-Fernández, María Rosario   +5 more
core   +2 more sources

Molecular dissection of the mechanism by which EWS/FLI expression compromises actin cytoskeletal integrity and cell adhesion in Ewing sarcoma. [PDF]

open access: yes, 2014
Ewing sarcoma is the second-most-common bone cancer in children. Driven by an oncogenic chromosomal translocation that results in the expression of an aberrant transcription factor, EWS/FLI, the disease is typically aggressive and micrometastatic upon ...
Beckerle, Mary C   +8 more
core   +1 more source

Optimizing tick artificial membrane feeding for Ixodes scapularis

open access: yesScientific Reports, 2023
Artificial membrane feeding (AMF) is a powerful and versatile technique with a wide range of applications in the study of disease vectors species. Since its first description, AMF has been under constant optimization and standardization for different ...
Melina Garcia Guizzo   +5 more
doaj   +1 more source

Phylogeny of Rhynchium and Its Related Genera (Hymenoptera: Eumeninae) Based on Universal Single-Copy Orthologs and Ultraconserved Elements

open access: yesInsects, 2023
The subfamily Eumeninae is a large group of fierce predatory insects that prey mainly on the larvae of Lepidoptera pests. Because of the highly similar morphologies of the genus Rhynchium and its related genera in the subfamily, including Rhynchium ...
Min Dai   +3 more
doaj   +1 more source

A conserved filamentous assembly underlies the structure of the meiotic chromosome axis. [PDF]

open access: yes, 2019
The meiotic chromosome axis plays key roles in meiotic chromosome organization and recombination, yet the underlying protein components of this structure are highly diverged.
Caballero, Iracema   +10 more
core   +4 more sources

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