Results 61 to 70 of about 600,320 (348)

Seamless, modular binary vectors for plant cell and molecular biology

Plant Biotechnology Journal
Summary In plants, genetic manipulations are commonly performed using Agrobacterium tumefaciens , a plant pathogen whose abilities as a ‘natural genetic engineer’ have been co‐opted for biotechnological applications. In the widely used binary vector systems,
Rory Greenhalgh, Wilson Horner, Katherine A. Klimpel, Snigdha Chatterjee, M. Regina Scarpin, Jacob O. Brunkard   +5 more
openaire   +3 more sources

Comparison of the standard WHO susceptibility tests and the CDC bottle bioassay for the determination of insecticide susceptibility in malaria vectors and their correlation with biochemical and molecular biology assays in Benin, West Africa [PDF]

Parasites & Vectors, 2013
The detection of insecticide resistance in natural populations of Anopheles vectors is absolutely necessary for malaria control. In the African region, the WHO insecticide susceptibility test is the most common method for assessing resistance status.
Aïzoun, Nazaire, Ossè, Razaki, Azondekon, Roseric, Alia, Roland, Oussou, Olivier, Gnanguenon, Virgile, Aikpon, Rock, Padonou, Gil, Akogbéto, Martin   +8 more
openaire   +2 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Genome Evolution of Wolbachia Strain wPip from the Culex pipiens Group [PDF]

, 2008
The obligate intracellular bacterium Wolbachia pipientis strain wPip induces cytoplasmic incompatibility (CI), patterns of crossing sterility, in the Culex pipiens group of mosquitoes.
Sinkins, Steven P., Sanders, Mandy J, Walker, T, Sebaihia, M., Quail, M. A., Quail, MA, Quail, Michael A., Sanders, MJ, Sebaihia, M, Parkhill, J, Thomson, N., Lord, Angela, Sanders, Susanne, Klasson, L, Sanders, Mandy J., Parkhill, J., Klasson, Lisa, Lord, A., Sanders, S, Klasson, L., Sinkins, Steven P, Lord, A, Sinkins, S. P., O'Neill, Scott L, Thomson, N, Quail, Michael A, Sebaihia, Mohammed, O'Neill, S. L., Parkhill, Julian, Earl, J, Sanders, S., Walker, Thomas, Thomson, Nicholas, O'Neill, Scott L., Earl, J., O'Neill, SL, Earl, Julie, Walker, T., Sinkins, SP, Sanders, M. J.   +39 more
core   +1 more source

Centrin-deficient Leishmania mexicana confers protection against Old World visceral leishmaniasis

npj Vaccines, 2022
Leishmaniasis is one of the top neglected tropical diseases with significant morbidity and mortality in low and middle-income countries (LMIC). However, this disease is also spreading in the developed world.
Subir Karmakar, Greta Volpedo, Wen-Wei Zhang, Patrick Lypaczewski, Nevien Ismail, Fabiano Oliveira, James Oristian, Claudio Meneses, Sreenivas Gannavaram, Shaden Kamhawi, Shinjiro Hamano, Jesus G. Valenzuela, Greg Matlashewski, Abhay R. Satoskar, Ranadhir Dey, Hira L. Nakhasi   +15 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Aspects of the molecular biology of potato virus Y [PDF]

, 1989
Aspects from the coat protein from Potato Virus Y (PVY) were investigated. Polyadenylated, full length RNA was isolated from purified preparations of two strains of the virus, PVYN and PVYC.
Hay, Joanne M.
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Gametocytes: insights gained during a decade of molecular monitoring [PDF]

, 2008
In vertebrate hosts, malaria parasites produce specialized male and female sexual stages (gametocytes). Soon after being taken up by a mosquito, gametocytes rapidly produce gametes and, once mated, they infect their vector and can be transmitted to new ...
Babiker, Hamza A., Reece, Sarah E., Schneider, Petra   +2 more
core   +1 more source