Results 61 to 70 of about 824,962 (311)

AAV Production Everywhere: A Simple, Fast, and Reliable Protocol for In‐house AAV Vector Production Based on Chloroform Extraction

Current Protocols in Neuroscience, 2020
Recombinant adeno‐associated virus (rAAV) is a mammalian virus that has been altered to be used as a gene delivery vehicle. Several changes to the viral genome have made them replication deficient so that this aspect of the viral infection cycle is under
M. Negrini, G. Wang, A. Heuer, T. Björklund, M. Davidsson   +4 more
semanticscholar   +1 more source

Structural dynamics of the plant hormone receptor ETR1 in a native‐like membrane environment

FEBS Letters, EarlyView.
The present study unveils the structural and signaling dynamics of ETR1, a key plant ethylene receptor. Using an optimized nanodisc system and solution NMR, we captured full‐length ETR1 in a native‐like membrane environment. Our findings reveal dynamic domain uncoupling and Cu(I)‐induced rigidification, providing the first evidence of metal‐triggered ...
Moritz Lemke, Nils Alexander Lakomek, Georg Groth   +2 more
wiley   +1 more source

Mechanistic basis for inhibition of the extended‐spectrum β‐lactamase GES‐1 by enmetazobactam and tazobactam

FEBS Letters, EarlyView.
Antimicrobial resistance (AMR) is of huge importance, resulting in over 1 million deaths each year. Here, we describe how a new drug, enmetazobactam, designed to help fight resistant bacterial diseases, inhibits a key enzyme (GES‐1) responsible for AMR. Our data show it is a more potent inhibitor than the related tazobactam, with high‐level computation
Michael Beer, Philip Hinchliffe, Marko Hanževački, Christopher R. Bethel, Catherine L. Tooke, Marc W. Van der Kamp, Krisztina M. Papp‐Wallace, Robert A. Bonomo, Stuart Shapiro, Adrian J. Mulholland, James Spencer   +10 more
wiley   +1 more source

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source

AAV Genome Interactions Important for Vector Production and Transduction.

Human Gene Therapy, 2020
Recombinant Adeno-Associated Virus (rAAV) has emerged as one of the most promising gene therapy delivery vectors. Development of these vectors took advantage of key features of the wild-type AAV virus (wtAAV), enabled by basic studies of the underlying ...
Anna C. Maurer, M. Weitzman
semanticscholar   +1 more source

Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1

FEBS Letters, EarlyView.
Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri, Andrea Lapucci, Giuseppe Orsomando, Nadia Raffaelli, Alberto Chiarugi, Daniela Buonvicino   +5 more
wiley   +1 more source

Coinfection With Trypanosoma brucei Confers Protection Against Cutaneous Leishmaniasis

Frontiers in Immunology, 2018
Infection with certain bacteria, parasites, and viruses alters the host immune system to Leishmania major influencing disease outcome. Here, we determined the outcome of a chronic infection with Trypanosoma brucei brucei on cutaneous leishmaniasis (CL ...
Lais Pereira, Fabiano Oliveira, Shannon Townsend, Sonia Metangmo, Claudio Meneses, Ian N. Moore, Claudia I. Brodskyn, Jesus G. Valenzuela, Stefan Magez, Stefan Magez, Shaden Kamhawi   +10 more
doaj   +1 more source

RAD50 missense variants differentially affect the DNA damage response and mitotic progression

FEBS Letters, EarlyView.
RAD50 incorporates into the MRN complex and initiates the DNA damage response. Furthermore, RAD50 promotes mitotic progression. RAD50 missense variants capable of forming an MRN complex supported the DNA damage response and mitotic features to different extents in complementation experiments, indicating these functions are separable and might impact ...
Hanna Redeker, Swantje Kebel, Lea Völkening, Anna Vatselia, Louisa Weinhold, Girmay Asgedom, Axel Schambach, Detlev Schindler, Thilo Dörk, Kristine Bousset   +9 more
wiley   +1 more source

Malnutrition exacerbates pathogenesis of Lutzomyia longipalpis sand fly-transmitted Leishmania donovani

Communications Biology
Visceral leishmaniasis (VL) is transmitted by Leishmania-infected sand fly bites and malnutrition is a known risk factor in human VL. Models using sand fly transmission or malnutrition promote parasite dissemination.
Eva Iniguez, Johannes Doehl, Pedro Cecilio, Tiago Donatelli Serafim, Caroline Percopo, Yvonne Rangel-Gonzalez, Somaditya Dey, Elvia J. Osorio, Patrick Huffcutt, Sofia Roitman, Claudio Meneses, Mara Short, Jesus G. Valenzuela, Peter C. Melby, Shaden Kamhawi   +14 more
doaj   +1 more source

Overcoming insecticide resistance in Anopheles mosquitoes by using faster-acting solid forms of deltamethrin

Malaria Journal, 2023
Background Controlling malaria-transmitting Anopheles mosquitoes with pyrethroid insecticides is becoming increasingly challenging because of widespread resistance amongst vector populations.
Jessica Carson, Bryan Erriah, Stephania Herodotou, Alexander G. Shtukenberg, Leilani Smith, Svetlana Ryazanskaya, Michael D. Ward, Bart Kahr, Rosemary Susan Lees   +8 more
doaj   +1 more source