Results 41 to 50 of about 269,913 (265)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Subsurface Structure in Japan Based on P and S waves Travel Time Analysis Using Genetic Algorithm in Japan Seismological Network

International Journal of Science and Engineering, 2014
Experiment to obtain the subsurface structure in Japan is conducted using seismograms analysis of earthquakes in Japan. All 101 data was used from events in 2012, selected by a maximum depth of 60 km and magnitude between 4.2 to 5.5 Mj.
A. M. Miftahul Huda, Bagus Jaya Santosa
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

P-wave crustal tomography of Greece with use of an accurate two-point ray tracer

Annals of Geophysics, 1997
The three-dimensional velocity structure of the crust in the Aegean sea and the surrounding regions (34.0º-42.OºN, 19.0ºE-29.0ºE) is investigated by inversion of about 10000 residuals of arrival times of P-wave from local events.
G. N. Stavrakakis, G. Karantonis, G. Drakatos   +2 more
doaj   +1 more source

Derivation of Engineering Design Criteria for Flow Field Around Intake Structure: A Numerical Simulation Study

Journal of Marine Science and Engineering, 2020
The primary environmental impact caused by seawater intake operation is marine life impingement resulting from the intake velocity. Environmental Protection Agency (EPA) of United State has regulated the use of velocity cap fitted at intake structures to
Lee Hooi Chie, Ahmad Khairi Abd Wahab
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Low-Velocity Impact Resistance of Double-Layer Folded Sandwich Structure

Machines, 2022
The folded sandwich structure might inevitably be impacted at low velocity when it is working, which will lead to the decline of the mechanical properties.
Yuechen Duan, Zhiren Zhan, Ting Zou, Ying Tie, Zhen Cui, Tingting Wang   +5 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Interstellar cloud structure: the statistics of centroid velocities [PDF]

Astronomy & Astrophysics, 2006
The investigation of the statistical properties of maps of line centroids has been used for almost 50 years, but there is still no general agreement on their interpretation. We try to quantify which properties of underlying turbulent velocity fields can be derived from centroid velocity maps, and we test conditions under which the scaling behaviour of ...
Ossenkopf, V., Esquivel, A., Lazarian, A., Stutzki, J.   +3 more
openaire   +2 more sources