Results 31 to 40 of about 352,568 (281)

Glyco-variant library of the versatile enzyme horseradish peroxidase [PDF]

Glycobiology, 2014
When the glycosylated plant enzyme horseradish peroxidase (HRP) is conjugated to specific antibodies, it presents a powerful tool for medical applications. The isolation and purification of this enzyme from plant is difficult and only gives low yields.
Capone, Simona, Pletzenauer, Robert, Maresch, Daniel, Metzger, Karl, Altmann, Friedrich, Herwig, Christoph, Spadiut, Oliver   +6 more
openaire   +2 more sources

Catabolic profiling of selective enzymes in the saccharification of non-food lignocellulose parts of biomass into functional edible sugars and bioenergy: An in silico bioprospecting

Journal of Advanced Veterinary and Animal Research, 2022
Objectives: The research aims to analyze the catabolic strength of different hydrolytic enzymes in assessing the biological conversion potential of lignocellulose parts of agricultural biomass wastes into functional edible sugars and biofuels. Materials
Parag Kumar Paul, Salauddin Al Azad, Mohammad Habibur Rahman, Mithila Farjana, Muhammad Ramiz Uddin, Dipta Dey, Shafi Mahmud, Tanzila Ismail Ema, Partha Biswas, Maliha Anjum, Ozifatun Jannat Akhi, Shahlaa Zernaz Ahmed   +11 more
doaj   +1 more source

Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences [PDF]

Journal of the American Chemical Society, 2022
White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous biocatalysts. VPs are attractive enzymes for research and industrial use but their recombinant production is extremely challenging.
Barber-Zucker, Shiran, Mindel, Vladimir, Garcia-Ruiz, Eva, Weinstein, Jonathan J., Alcalde, Miguel, Fleishman, Sarel J.   +5 more
openaire   +3 more sources

Peroxidase profiling reveals genetic linkage between peroxidase gene clusters and basal host and non-host resistance to rusts and mildew in barley [PDF]

, 2010
Background - Higher plants possess a large multigene family encoding secreted class III peroxidase (Prx) proteins. Peroxidases appear to be associated with plant disease resistance based on observations of induction during disease challenge and the ...
Stam, Piet, Linden, C. Gerard van der, Ana M. González, Stam Piet, Kohutova Zuzana, Thierry C Marcel, Marcel, T. C., Niks Rients E., Rients E Niks, Kohutova, Z., Ana M González, Zuzana Kohutova, Stam, P., González Fernández, Ana María, C Gerard van der Linden, Rients E. Niks, van der Linden, C.G., Marcel, T.C., van der Linden C. Gerard, C. Gerard van der Linden, Niks, R. E., Kohutova, Zuzana, Niks, R.E., González Ana M., Gonzalez, A.M., Piet Stam, Marcel Thierry C., Thierry C. Marcel, Linden, C.G., van der   +28 more
core   +1 more source

Acute and persistent Mycobacterium tuberculosis infections depend on the thiol peroxidase TpX. [PDF]

, 2009
The macrophage is the natural niche of Mycobacterium tuberculosis infection. In order to combat oxidative and nitrosative stresses and persist in macrophages successfully, M. tuberculosis is endowed with a very efficient antioxidant complex.
Hu, Y, Yanmin Hu, Anthony R M Coates, Anthony R. M. Coates, Coates, AR   +4 more
core   +2 more sources

Lignin-degrading peroxidases in white-rot fungus Trametes hirsuta 072. Absolute expression quantification of full multigene family. [PDF]

PLoS ONE, 2017
Ligninolytic heme peroxidases comprise an extensive family of enzymes, which production is characteristic for white-rot Basidiomycota. The majority of fungal heme peroxidases are encoded by multigene families that differentially express closely related ...
Daria V Vasina, Konstantin V Moiseenko, Tatiana V Fedorova, Tatiana V Tyazhelova   +3 more
doaj   +1 more source

Mushroom Ligninolytic Enzymes―Features and Application of Potential Enzymes for Conversion of Lignin into Bio-Based Chemicals and Materials

Applied Sciences, 2021
Mushroom ligninolytic enzymes are attractive biocatalysts that can degrade lignin through oxido-reduction. Laccase, lignin peroxidase, manganese peroxidase, and versatile peroxidase are the main enzymes that depolymerize highly complex lignin structures ...
Seonghun Kim
doaj   +1 more source

The use of soybean peroxidase in amperometric biosensors [PDF]

, 2004
Traditionally Horseradish peroxidase (HRP) the archetypal class III plant peroxidase has been investigated with respect to chemical modifications, organic solvent tolerance studies, reaction kinetics, kinetics in organic solvents, immunohistochemical and
Carolan, Neil
core   +2 more sources

Characterization of a Dye-Decolorizing Peroxidase from Irpex lacteus Expressed in Escherichia coli: An Enzyme with Wide Substrate Specificity Able to Transform Lignosulfonates

Journal of Fungi, 2021
A dye-decolorizing peroxidase (DyP) from Irpex lacteus was cloned and heterologously expressed as inclusion bodies in Escherichia coli. The protein was purified in one chromatographic step after its in vitro activation.
Laura Isabel de Eugenio, Rosa Peces-Pérez, Dolores Linde, Alicia Prieto, Jorge Barriuso, Francisco Javier Ruiz-Dueñas, María Jesús Martínez   +6 more
doaj   +1 more source

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase [PDF]

Biochemical Journal, 2011
The VPs (versatile peroxidases) secreted by white-rot fungi are involved in the natural decay of lignin. In the present study, a fusion gene containing the VP from Pleurotus eryngii was subjected to six rounds of directed evolution, achieving a level of secretion in Saccharomyces cerevisiae (21 mg/l) as yet unseen for any ligninolytic peroxidase.
García-Ruiz E, González-Pérez D, Ruiz-Dueñas FJ, Martínez AT, Alcalde M   +4 more
openaire   +3 more sources