Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy [PDF]
Molecular Biomedicine, 2022 Intracellular vesicle trafficking is the fundamental process to maintain the homeostasis of membrane-enclosed organelles in eukaryotic cells. These organelles transport cargo from the donor membrane to the target membrane through the cargo containing ...
Lele Cui +9 more
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Cooperative inhibition of SNARE-mediated vesicle fusion by α-synuclein monomers and oligomers [PDF]
Scientific Reports, 2021 The primary hallmark of Parkinson's disease (PD) is the generation of Lewy bodies of which major component is α-synuclein (α-Syn). Because of increasing evidence of the fundamental roles of α-Syn oligomers in disease progression, α-Syn oligomers have ...
Gyeongji Yoo +4 more
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SNARE Regulatory Proteins in Synaptic Vesicle Fusion and Recycling. [PDF]
Front Mol Neurosci, 2021 Membrane fusion is a universal feature of eukaryotic protein trafficking and is mediated by the soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) family.
Sauvola CW, Littleton JT.
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Requirement of Cholesterol for Calcium‐Dependent Vesicle Fusion by Strengthening Synaptotagmin‐1‐Induced Membrane Bending [PDF]
Advanced Science, 2023 Cholesterol is essential for neuronal activity and function. Cholesterol depletion in the plasma membrane impairs synaptic transmission. However, the molecular mechanisms by which cholesterol deficiency leads to defects in vesicle fusion remain poorly ...
Houda Yasmine Ali Moussa +6 more
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Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion [PDF]
eLife, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Jiajie Diao +13 more
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The effect of hydrophilic ionic liquids 1-ethyl-3-methylimidazolium lactate and choline lactate on lipid vesicle fusion. [PDF]
PLoS ONE, 2013 Ionic liquids (ILs) are room-temperature molten salts that have applications in both physical sciences and more recently in the purification of proteins and lipids, gene transfection and sample preparation for electron microscopy (EM) studies ...
Eri H Hayakawa +5 more
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Membrane fusion of secretory vesicles and liposomes Two different types of fusion [PDF]
, 1981 Secretory vesicles isolated from adrenal medulla were found to fuse in vitro in response to incubation with Ca2+. Intervesicular fusion was detected by electron microscopy and was indicated by the appearance of twinned vesicles in freeze-fractured ...
Roland Ekerdt +2 more
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Single-vesicle intensity and colocalization fluorescence microscopy to study lipid vesicle fusion, fission, and lipid exchange [PDF]
Frontiers in Molecular Neuroscience, 2022 Interactions of lipid vesicles play important roles in a large variety of functions and dysfunctions in the human body. Vital for several biochemical functions is the interaction between monomeric proteins and lipid membranes, and the induced phenomena ...
Alexandra Andersson +6 more
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eLife, 2014 Previously we showed that fast Ca2+-triggered vesicle fusion with reconstituted neuronal SNAREs and synaptotagmin-1 begins from an initial hemifusion-free membrane point contact, rather than a hemifusion diaphragm, using a single vesicle–vesicle lipid ...
Ying Lai +6 more
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Modulating vesicle priming reveals that vesicle immobilization is necessary but not sufficient for fusion-competence. [PDF]
PLoS ONE, 2008 In neurons and neuroendocrine cells, docked vesicles need to undergo priming to become fusion competent. Priming is a multi-step process that was shown to be associated with vesicle immobilization.
Ofer Yizhar, Uri Ashery
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