Nature Structural & Molecular Biology, 2008 The core of the neurotransmitter release machinery is formed by SNARE complexes, which bring the vesicle and plasma membranes together and are key for fusion, and by Munc18-1, which controls SNARE-complex formation and may also have a direct role in fusion.
Josep, Rizo, Christian, Rosenmund
openaire +2 more sources
Modulating vesicle priming reveals that vesicle immobilization is necessary but not sufficient for fusion-competence. [PDF]
PLoS ONE, 2008 In neurons and neuroendocrine cells, docked vesicles need to undergo priming to become fusion competent. Priming is a multi-step process that was shown to be associated with vesicle immobilization.
Ofer Yizhar, Uri Ashery
doaj +1 more source
Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense. [PDF]
Mol Plant PatholFang Z +8 more
europepmc +3 more sources
The effect of hydrophilic ionic liquids 1-ethyl-3-methylimidazolium lactate and choline lactate on lipid vesicle fusion. [PDF]
PLoS ONE, 2013 Ionic liquids (ILs) are room-temperature molten salts that have applications in both physical sciences and more recently in the purification of proteins and lipids, gene transfection and sample preparation for electron microscopy (EM) studies ...
Eri H Hayakawa +5 more
doaj +1 more source
Genome-wide identification, phylogeny and expression profile of vesicle fusion components in Verticillium dahliae. [PDF]
PLoS ONE, 2013 Vesicular trafficking plays a crucial role in protein localization and movement, signal transduction, and multiple developmental processes in eukaryotic cells.
Xue Yang +5 more
doaj +1 more source
Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis
eLife, 2020 Calcium (Ca2+)-evoked release of neurotransmitters from synaptic vesicles requires mechanisms both to prevent un-initiated fusion of vesicles (clamping) and to trigger fusion following Ca2+-influx. The principal components involved in these processes are
Sathish Ramakrishnan +4 more
doaj +1 more source
Vesicle Shrinking and Enlargement Play Opposing Roles in the Release of Exocytotic Contents
Cell Reports, 2020 Summary: For decades, two fusion modes were thought to control hormone and transmitter release essential to life; one facilitates release via fusion pore dilation and flattening (full collapse), and the other limits release by closing a narrow fusion ...
Wonchul Shin +11 more
doaj +1 more source
Atomic-resolution simulations predict a transition state for vesicle fusion defined by contact of a few lipid tails. [PDF]
PLoS Computational Biology, 2010 Membrane fusion is essential to both cellular vesicle trafficking and infection by enveloped viruses. While the fusion protein assemblies that catalyze fusion are readily identifiable, the specific activities of the proteins involved and nature of the ...
Peter M Kasson +2 more
doaj +1 more source
Acute Complexin Knockout Abates Spontaneous and Evoked Transmitter Release
Cell Reports, 2019 Summary: SNARE-mediated synaptic vesicle (SV) fusion is controlled by multiple regulatory proteins that determine neurotransmitter release efficiency.
Francisco José López-Murcia +4 more
doaj +1 more source
An active tethering mechanism controls the fate of vesicles
Nature Communications, 2021 Molecular tethers physically bridge transport vesicles to their target membranes as a prerequisite step for fusion. Here the authors control vesicle tethering using optogenetic approaches to study the interplay between vesicle tethering and fusion.
Seong J. An +6 more
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