Coordinated oscillations in cortical actin and Ca2+ correlate with cycles of vesicle secretion. [PDF]
, 2012 The actin cortex both facilitates and hinders the exocytosis of secretory granules. How cells consolidate these two opposing roles was not well understood. Here we show that antigen activation of mast cells induces oscillations in Ca(2+) and PtdIns(4,5)P(
Meyer, T, Wollman, R
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Molecular Mechanisms of Vesicle Fusion [PDF]
Biophysical Journal, 2009 We have performed large scale molecular dynamics simulations using coarse-grained lipid models to study the fusion of vesicles. Our earlier work showed that lipid tail splay played an important role in the first lipids to cross from one vesicle to another.
Mark J. Stevens, Dina T. Mirijanian
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A resting pool of vesicles is responsible for spontaneous vesicle fusion at the synapse [PDF]
Nature Neuroscience, 2009 Synapses relay information through the release of neurotransmitters stored in presynaptic vesicles. The identity, kinetics and location of the vesicle pools that are mobilized by neuronal activity have been studied using a variety of techniques.
Ben Fredj, Naila, Burrone, Juan
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Molecular control of compound exocytosis: A key role for VAMP8 [PDF]
, 2012 Exocytosis is the process of fusion of a membrane-bound vesicle with the cell membrane and subsequent release of the vesicle content to the outside.
Gaisano, Herbert, Thorn, Peter
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Roles of Cholesterol in Vesicle Fusion and Motion [PDF]
Biophysical Journal, 2009 Although it is well established that exocytosis of neurotransmitters and hormones is highly regulated by numerous secretory proteins, such as SNARE proteins, there is an increasing appreciation of the importance of the chemophysical properties and organization of membrane lipids to various aspects of the exocytotic program.
Zhang, J., Xue, R., Ong, W.-Y., Chen, P.
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Membrane Fusion in Vesicles of Oligomerizable Lipids [PDF]
Biophysical Journal, 1999 Membrane fusion has been examined in a model system of small unilamellar vesicles of synthetic lipids that can be oligomerized through the lipid headgroups. The oligomerization can be induced either in both bilayer leaflets or in the inner leaflet exclusively.
W. D. Weringa +2 more
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An active tethering mechanism controls the fate of vesicles
Nature Communications, 2021 Molecular tethers physically bridge transport vesicles to their target membranes as a prerequisite step for fusion. Here the authors control vesicle tethering using optogenetic approaches to study the interplay between vesicle tethering and fusion.
Seong J. An +6 more
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VAMP721 conformations unmask an extended motif for K+ channel binding and gating control [PDF]
, 2016 Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins play a major role in membrane fusion and contribute to cell expansion, signaling, and polar growth in plants.
Blatt, Mike R. +4 more
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Snare Mediated Vesicle Fusion in Supported Membranes: Comparing Synaptic Vesicles with Reconstituted Vesicles [PDF]
Biophysical Journal, 2012 In vitro reconstitution experiments have played an essential role in a large body of research on SNARE-mediated membrane fusion. We and others have developed single vesicle assays to gain more detailed insight into the kinetics of vesicle docking and fusion.
Reinhard Jahn +5 more
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LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate [PDF]
, 2016 Background Lrrk2, a gene linked to Parkinson\u2019s disease, encodes a large scaffolding protein with kinase and GTPase activities implicated in vesicle and cytoskeletal-related processes.
Arrigoni, Giorgio +15 more
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