Results 31 to 40 of about 131,357 (307)
Epigallocatechin gallate inhibits SNARE‐dependent membrane fusion by blocking trans‐SNARE assembly
FEBS Open Bio, 2022 Insulin secretion is a signal‐triggered process that requires membrane fusion between the secretory granules and plasma membrane in pancreatic β cells. The exocytosis of insulin is mediated by target‐soluble N‐ethylmaleimide sensitive factor attachment ...
Min Zhu +4 more
doaj +1 more source
SNARE Zippering Is Suppressed by a Conformational Constraint that Is Removed by v-SNARE Splitting
Cell Reports, 2021 Summary: Intracellular vesicle fusion is catalyzed by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). Vesicle-anchored v-SNAREs pair with target membrane-associated t-SNAREs to form trans-SNARE complexes, releasing free ...
Yinghui Liu +5 more
doaj +1 more source
Exocyst dynamics during vesicle tethering and fusion [PDF]
Nature Communications, 2018 Abstract The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma membrane prior to fusion. Exocyst assembly and delivery mechanisms remain unclear, especially in mammalian cells. Here we tagged multiple endogenous exocyst subunits with sfGFP or Halo using Cas9 gene-editing, to create single and double
Ahmed, Syed Mukhtar +5 more
openaire +3 more sources
Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells
eLifeMembranes undergo various patterns of deformation during vesicle fusion, but how this membrane deformation is regulated and contributes to fusion remains unknown.
Seiichi Koike +6 more
doaj +1 more source
Membrane Fusion in Vesicles of Oligomerizable Lipids [PDF]
Biophysical Journal, 1999 Membrane fusion has been examined in a model system of small unilamellar vesicles of synthetic lipids that can be oligomerized through the lipid headgroups. The oligomerization can be induced either in both bilayer leaflets or in the inner leaflet exclusively.
Ravoo, B.J +2 more
openaire +4 more sources
Temporal Separation of Vesicle Release from Vesicle Fusion during Exocytosis [PDF]
Journal of Biological Chemistry, 2002 During exocytosis, vesicles in secretory cells fuse with the cellular membrane and release their contents in a Ca2+-dependent process. Release occurs initially through a fusion pore, and its rate is limited by the dissociation of the matrix-associated contents.
Kevin P, Troyer, R Mark, Wightman
openaire +2 more sources
eLife, 2014 Previously we showed that fast Ca2+-triggered vesicle fusion with reconstituted neuronal SNAREs and synaptotagmin-1 begins from an initial hemifusion-free membrane point contact, rather than a hemifusion diaphragm, using a single vesicle–vesicle lipid ...
Ying Lai +6 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 Impaired insulin release is a hallmark of type 2 diabetes and is closely related to chronically elevated glucose concentrations, known as “glucotoxicity.” However, the molecular mechanisms by which glucotoxicity impairs insulin secretion remain poorly ...
Guoyi Yang +9 more
doaj +1 more source
Supported Lipid Bilayer Formation: Beyond Vesicle Fusion
Langmuir, 2020 Supported lipid bilayers (SLBs) are cell-membrane-mimicking platforms that can be formed on solid surfaces and integrated with a wide range of surface-sensitive measurement techniques. SLBs are useful for unravelling details of fundamental membrane biology and biophysics as well as for various medical, biotechnology, and environmental science ...
Joshua A. Jackman, Nam-Joon Cho
openaire +4 more sources
Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion
eLife, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Jiajie Diao +13 more
doaj +1 more source