Results 101 to 110 of about 221,902 (249)

Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics

open access: yesFrontiers in Veterinary Science
The interaction between viral proteins and host proteins plays a crucial role in the process of virus infecting cells. Tags such as HA, His, and Flag do not interfere with the function of fusion proteins and are commonly used to study protein–protein ...
Ruiwei Wang   +12 more
doaj   +1 more source

Light‐Assisted 3D Printing Techniques and Photocrosslinking Strategies: Recent Advances in Musculoskeletal Tissue Engineering

open access: yesAdvanced Engineering Materials, EarlyView.
In this review, the current state of light‐assisted 3D printing as it pertains to engineering musculoskeletal tissues including bone, cartilage, skeletal muscle, tendon, and ligaments is summarized. Common printing techniques, photoreactive materials, and study design choices are compiled and reviewed.
Meagan Morgan, Bin Zhang, Roger Narayan
wiley   +1 more source

Swelling‐Programmed Topographical Guidance for Dynamic Spheroid Self‐Assembly via a Mechanochemical Hydrogel Niche

open access: yesAdvanced Functional Materials, EarlyView.
A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam   +8 more
wiley   +1 more source

Exploring an Alternative to mRNA Vaccine Cold Chain Storage: MRNA‐Lipid Nanoparticle Stability When Dried in a Polymer Matrix

open access: yesAdvanced Functional Materials, EarlyView.
The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett   +19 more
wiley   +1 more source

Receptor-Activated Binding of Viral Fusion Proteins to Target Membranes

open access: yes, 2003
This chapter describes three assays to monitor receptor-induced association of the envelope glycoprotein (EnvA) of avian sarcoma/leukosis virus (ASLV) with target bilayers: (1) the original assay for monitoring binding of the EnvA ectodomain (EnvA-PI) to target membranes (liposomes), (2) a modified and miniaturized EnvA-PI-liposome binding assay, and ...
Earp, Laurie J   +3 more
openaire   +2 more sources

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

open access: yesAdvanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj   +23 more
wiley   +1 more source

Newcastle Disease: A Review [PDF]

open access: yesEgyptian Journal of Animal Health
Virulent strains of Newcastle disease virus (NDV) can cause severe damage to tissues in the gastrointestinal tract, respiratory and reproductive organs, in addition to neurological disorders and high mortality in poultry.
doaj   +1 more source

Engineering Nanoplatforms for Alzheimer's Disease Detection via Biomolecular Corona Proteomic and Lipidomic Profiling

open access: yesAdvanced Healthcare Materials, EarlyView.
Engineered nanoparticles capture disease‐specific biomolecular coronas that uncover hidden molecular features of Alzheimer's disease. Combined proteomic and lipidomic analyses reveal a characteristic shift in ribosomal machinery and energy metabolism, generating a multiomic fingerprint that supports accurate disease detection and opens new ...
Antonietta Greco   +7 more
wiley   +1 more source

A Modular Liver–Microbial Microfluidic Platform to Evaluate Therapeutic and Adverse Effects of Microbial Metabolites

open access: yesAdvanced Healthcare Materials, EarlyView.
This study introduces a modular microfluidic platform that connects liver cells with live microbes to mimic key aspects of the gut–liver interaction in steatosis. Using this system, both microbial metabolites and live bacteria significantly reduced fat accumulation in liver cells, but live microbes triggered inflammation and broader metabolic changes ...
Hsih‐Yin Tan   +7 more
wiley   +1 more source

Home - About - Disclaimer - Privacy