Results 191 to 200 of about 35,570 (216)
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Motif C in nonstructural protein 5 of duck Tembusu virus is essential for viral proliferation
Veterinary Microbiology, 2021Nonstructural protein 5 (NS5) is required for duck Tembusu virus (DTMUV) genome replication, and the GDD motif in motif C is considered the hallmark of RdRp. However, the role of GDD-adjacent amino acids in motif C in viral proliferation is still unclear. To explore the role of motif C in the virus life cycle, DTMUV infectious clones and replicons were
Lu Tong +13 more
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Archives of Virology, 2015
Rice grassy stunt virus (RGSV), a member of the genus Tenuivirus, causes serious rice disease in Southeast Asian countries. In this study, a green fluorescent protein (GFP)-based transient expression assay was conducted to show that p5, encoded on RNA5 in the viral sense, is a viral suppressor of RNA silencing (VSR). Protein-protein interactions (PPIs)
Chao, Zhang +9 more
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Rice grassy stunt virus (RGSV), a member of the genus Tenuivirus, causes serious rice disease in Southeast Asian countries. In this study, a green fluorescent protein (GFP)-based transient expression assay was conducted to show that p5, encoded on RNA5 in the viral sense, is a viral suppressor of RNA silencing (VSR). Protein-protein interactions (PPIs)
Chao, Zhang +9 more
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Archives of Virology, 1990
In this report, the molecular weight and antigenicity of the proteins of a porcine enteric calicivirus (PEC) were characterized. The PEC virions were purified from intestinal contents of infected pigs and from infected cell culture lysates. The average buoyant density of the purified virus was 1.37 gm/cm3 in cesium chloride.
A V, Parwani, L J, Saif, S Y, Kang
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In this report, the molecular weight and antigenicity of the proteins of a porcine enteric calicivirus (PEC) were characterized. The PEC virions were purified from intestinal contents of infected pigs and from infected cell culture lysates. The average buoyant density of the purified virus was 1.37 gm/cm3 in cesium chloride.
A V, Parwani, L J, Saif, S Y, Kang
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Positive effect of the hepatitis C virus nonstructural 5A protein on viral multiplication
Archives of Virology, 2004Hepatitis C virus infection (HCV), is a major cause of liver disease worldwide, and are frequently resistant to the interferon alpha treatment. The nonstructural (NS) 5A protein of HCV has been proposed to be involved in this resistance. Additional studies have pointed out a role for NS5A in several other cellular interactions as well as an important ...
D, Bonte +6 more
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The Sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis
Virology, 1992An in vitro transcription system for paramyxoviruses is described, in which polymerase-free templates are combined with cell extracts containing polymerase made in vivo via transfected plasmids. Both P and L are required for polymerase activity, and both must be coexpressed for optimum activity.
Curran J, Marq JB, Kolakofsky D
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Journal of Infectious Diseases, 1992
Subgenomic hepatitis A virus (HAV) RNA sequences were translated in vitro to produce proteins representing the structural (P1) and nonstructural (P2 and P3) domains of the viral polyprotein. These proteins were used as antigens to detect the presence of antibodies in sera from acute and convalescent humans and an experimentally infected chimpanzee. All
E Ehrenfeld +2 more
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Subgenomic hepatitis A virus (HAV) RNA sequences were translated in vitro to produce proteins representing the structural (P1) and nonstructural (P2 and P3) domains of the viral polyprotein. These proteins were used as antigens to detect the presence of antibodies in sera from acute and convalescent humans and an experimentally infected chimpanzee. All
E Ehrenfeld +2 more
exaly +3 more sources
Reovirus Nonstructural Protein σNS Acts as an RNA Stability Factor Promoting Viral Genome Replication [PDF]
Journal of Virology, 2018 Following infection, viruses synthesize nonstructural proteins that mediate viral replication and promote dissemination. Viruses from the family Reoviridae encode nonstructural proteins that are required for the ...
Jonathan Knowlton, Roni Lahr, Liya Hu
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Archives of Virology, 2009
Bovine viral diarrhea virus (BVDV) nonstructural protein 5A (NS5A) is one the least studied of the BVDV proteins. Therefore, to develop a tool for unraveling the functions performed by BVDV NS5A, monoclonal antibodies (MAbs) were generated by fusion of myeloma cells with spleen cells from mice immunized with recombinant E.
Muhammad Atif, Zahoor +6 more
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Bovine viral diarrhea virus (BVDV) nonstructural protein 5A (NS5A) is one the least studied of the BVDV proteins. Therefore, to develop a tool for unraveling the functions performed by BVDV NS5A, monoclonal antibodies (MAbs) were generated by fusion of myeloma cells with spleen cells from mice immunized with recombinant E.
Muhammad Atif, Zahoor +6 more
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Molecular characterization of rubella virus nonstructural proteins and viral RNA synthesis
2009Rubella virus (RV) is a single-strand positive RNA virus. Its 9762-nucleotide (nt) genomic RNA contains two large open readings frames (ORFs) at the 5'- and 3'-termini, encoding nonstructural and structural proteins, respectively. The nonstructural proteins (NSPs) are first translated as a 200-kDa polyprotein (p200), which undergoes a single ...
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The American Journal of Tropical Medicine and Hygiene, 2009
Previous attempts to define dengue virus (DENV) tropism in human autopsy tissues have detected DENV antigens that are abundant in circulation during severe dengue, and thus may be present in uninfected cells. To better define DENV tropism, we performed immunostaining for the DENV2 nonstructural protein 3 (NS3) in humans and in a mouse model of DENV ...
Scott J, Balsitis +7 more
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Previous attempts to define dengue virus (DENV) tropism in human autopsy tissues have detected DENV antigens that are abundant in circulation during severe dengue, and thus may be present in uninfected cells. To better define DENV tropism, we performed immunostaining for the DENV2 nonstructural protein 3 (NS3) in humans and in a mouse model of DENV ...
Scott J, Balsitis +7 more
openaire +2 more sources