Results 1 to 10 of about 12,322 (192)

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies. [PDF]

PLoS ONE, 2021
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize.
Lee A Armstrong, Sven M Lange, Virginia Dee Cesare, Stephen P Matthews, Raja Sekhar Nirujogi, Isobel Cole, Anthony Hope, Fraser Cunningham, Rachel Toth, Rukmini Mukherjee, Denisa Bojkova, Franz Gruber, David Gray, Paul G Wyatt, Jindrich Cinatl, Ivan Dikic, Paul Davies, Yogesh Kulathu   +17 more
doaj   +5 more sources

Identification of a non-canonical G3BP-binding sequence in a Mayaro virus nsP3 hypervariable domain

Frontiers in Cellular and Infection Microbiology, 2022
Ras-GTPase-activating SH3 domain-binding-proteins 1 (G3BP1) and 2 (G3BP2) are multifunctional RNA-binding proteins involved in stress granule nucleation, previously identified as essential cofactors of Old World alphaviruses.
Laurence Briant, William Bakhache, Patrick Eldin   +2 more
exaly   +4 more sources

Alphavirus nsP3 organizes into tubular scaffolds essential for infection and the cytoplasmic granule architecture

Nature Communications
Alphaviruses, such as chikungunya virus (CHIKV), are mosquito-borne viruses that represent a significant threat to human health due to the current context of global warming.
Vasiliya Kril, Michael Hons, Celine Amadori, Claire Zimberger, Laurine Couture, Yara Bouery, Julien Burlaud-Gaillard, Andrei Karpov, Denis Ptchelkine, Alexandra L. Thienel, Beate M. Kümmerer, Ambroise Desfosses, Rhian Jones, Philippe Roingeard, Laurent Meertens, Ali Amara, Juan Reguera   +16 more
doaj   +3 more sources

Key residues in SARS-CoV-2 NSP3 hypervariable region are necessary to modulate early stress granule activity [PDF]

Journal of Virology
Antagonism of the host responses that limits viral replication is critical to the success of infection. Recently, we identified that the hypervariable region (HVR) of SARS-CoV-2 NSP3 binds to FXR1 and disrupts stress granule formation during the early ...
R. Elias Alvarado, Kumari G. Lokugamage, Dimitriya Garvanska, Leah K. Estes, Yani Ahearn, Alyssa M. McLeland, Arian Moayyed, Jennifer Chen, Blanca Lopez Mendez, Jessica A. Plante, Kenneth S. Plante, Bryan A. Johnson, Jakob Nilsson, Vineet D. Menachery   +13 more
doaj   +3 more sources

The DMV pore-forming TM2–Y region of SARS-CoV-2 nsp3 exhibits structural conservation beyond the coronavirus family [PDF]

Journal of Virology
Positive-strand RNA viruses remodel host intracellular membranes to form replication organelles in the cytoplasm. SARS-CoV-2 and other coronaviruses, members of the Nidovirales order, replicate within double-membrane vesicles (DMVs) derived from the ...
Alexandra Pozhidaeva, Jeffrey C. Hoch, Yulia Pustovalova   +2 more
doaj   +3 more sources

Heterodimerization of PRRSV replicase membrane proteins nsp2 and nsp3 regulates their cytoplasmic tail binding to viral RdRp domain for sgRNA synthesis [PDF]

Journal of Virology
Recruitment of viral RNA polymerase to replicase membrane proteins is a critical step for assembly of the replication and transcription complex (RTC) during the replication of positive-stranded RNA viruses.
Xueyan Liu, Yunhao Hu, Qiongqiong Zhou, Peng Gao, Yongning Zhang, Lei Zhou, Xinna Ge, Xin Guo, Jun Han, Hanchun Yang   +9 more
doaj   +3 more sources

Alphavirus nsP3 as a multifunctional orchestrator of virus-host interplay [PDF]

Journal of Virology
Alphaviruses can cause polyarthralgia and encephalitis and pose an escalating global health threat to humans and animals. They are transmitted to vertebrates mainly by infected mosquitoes, leading to host alternation that drives an evolutionary arms race
Ana Alice Pimenta-Pereira, Bailey Lubinski, Rafael Kroon Campos   +2 more
doaj   +2 more sources

Nsp3-N interactions are critical for SARS-CoV-2 fitness and virulence

Proceedings of the National Academy of Sciences of the United States of America, 2023
Significance SARS-CoV-2, the causative agent of COVID-19, encodes several proteins not present in other coronaviruses. SARS-Unique Domain (SUD), a domain in the nonstructural protein 3 (nsp3) of coronavirus, is present only in Sarbecovirus, a subgenus of
Lok Yin Roy Wong, Nicholas Schnicker, David K Meyerholz   +2 more
exaly   +2 more sources

Discovery and Development Strategies for SARS-CoV-2 NSP3 Macrodomain Inhibitors

Pathogens, 2023
The worldwide public health and socioeconomic consequences caused by the COVID-19 pandemic highlight the importance of increasing preparedness for viral disease outbreaks by providing rapid disease prevention and treatment strategies.
Tryfon Zarganes - Tzitzikas, Paul Brennan, Ivan Ahel   +2 more
exaly   +2 more sources

Structural and functional analyses of SARS-CoV-2 Nsp3 and its specific interactions with the 5’ UTR of the viral genome

Microbiology Spectrum
Non-structural protein 3 (Nsp3) is the largest open reading frame encoded in the SARS-CoV-2 genome, essential for the formation of double-membrane vesicles (DMV) wherein viral RNA replication occurs.
Sofia Lemak, Tatiana Skarina, Deepak T. Patel, Peter J. Stogios, Alexei Savchenko   +4 more
doaj   +2 more sources