Mutation of CD2AP and SH3KBP1 Binding Motif in Alphavirus nsP3 Hypervariable Domain Results in Attenuated Virus [PDF]
Viruses, 2018 Infection by Chikungunya virus (CHIKV) of the Old World alphaviruses (family Togaviridae) in humans can cause arthritis and arthralgia. The virus encodes four non-structural proteins (nsP) (nsP1, nsp2, nsP3 and nsP4) that act as subunits of the virus ...
Margit Mutso +13 more
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Key residues in SARS-CoV-2 NSP3 hypervariable region are necessary to modulate early stress granule activity [PDF]
Journal of VirologyAntagonism of the host responses that limits viral replication is critical to the success of infection. Recently, we identified that the hypervariable region (HVR) of SARS-CoV-2 NSP3 binds to FXR1 and disrupts stress granule formation during the early ...
R. Elias Alvarado +13 more
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SNAP-tagged Chikungunya Virus Replicons Improve Visualisation of Non-Structural Protein 3 by Fluorescence Microscopy [PDF]
Scientific Reports, 2017 Chikungunya virus (CHIKV), a mosquito-borne alphavirus, causes febrile disease, muscle and joint pain, which can become chronic in some individuals. The non-structural protein 3 (nsP3) plays essential roles during infection, but a complete understanding ...
Roland Remenyi +4 more
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Identification of a non-canonical G3BP-binding sequence in a Mayaro virus nsP3 hypervariable domain
Frontiers in Cellular and Infection Microbiology, 2022 Ras-GTPase-activating SH3 domain-binding-proteins 1 (G3BP1) and 2 (G3BP2) are multifunctional RNA-binding proteins involved in stress granule nucleation, previously identified as essential cofactors of Old World alphaviruses.
Patrick Eldin
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SARS-CoV-2 nonstructural protein 3 remodels the phosphorylation of target proteins via protein-protein interactions [PDF]
Microbiology SpectrumSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), triggered a global pandemic with a significant impact on human health. The molecular basis of its pathogenicity remains incompletely
Hui Yang +3 more
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Viruses, 2021 Alphavirus non-structural proteins 1–4 (nsP1, nsP2, nsP3, and nsP4) are known to be crucial for alphavirus RNA replication and translation. To date, nsP3 has been demonstrated to mediate many virus–host protein–protein interactions in several fundamental
Nurshariza Abdullah +2 more
exaly +3 more sources
Heliyon, 2020 Literature reported that nsp3 of CHIKV is an important target for the designing of drug as it involves in the replication, survival etc. Herein, about eighteen million molecules available in the ZINC database are filtered against nsp3 using RASPD.
Mahendra Kumar Meena +2 more
exaly +3 more sources
A structural roadmap for the formation of the coronavirus nsp3/nsp4 double membrane vesicle pore and its implications for polyprotein processing and replication/transcription [PDF]
Journal of VirologyCoronavirus replication is understood to occur within double membrane vesicles (DMVs) that arise during viral infection. Prior work has determined that these DMVs have characteristic pores formed from the non-structural viral proteins nsp3 and nsp4 ...
Jason K. Perry +3 more
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Structural and functional analyses of SARS-CoV-2 Nsp3 and its specific interactions with the 5’ UTR of the viral genome [PDF]
Microbiology SpectrumNon-structural protein 3 (Nsp3) is the largest open reading frame encoded in the SARS-CoV-2 genome, essential for the formation of double-membrane vesicles (DMV) wherein viral RNA replication occurs.
Sofia Lemak +4 more
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Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing [PDF]
Viruses, 2018 Positive-strand RNA viruses, such as coronaviruses, induce cellular membrane rearrangements during replication to form replication organelles allowing for efficient viral RNA synthesis.
Nicole Doyle +7 more
doaj +5 more sources