A Gaussia luciferase reporter assay for the evaluation of coronavirus Nsp5/3CLpro activity
Scientific ReportsHuman coronaviruses (hCoVs) infect millions of people every year. Among these, MERS, SARS-CoV-1, and SARS-CoV-2 caused significant morbidity and mortality and their emergence highlights the risk of possible future coronavirus outbreaks.
Rayhane Nchioua
exaly +4 more sources
Swine acute diarrhea syndrome coronavirus nsp5 induces apoptosis by targeting GATA zinc finger domain-containing protein 2A (GATAD2A/p66α) [PDF]
mBioSwine acute diarrhea syndrome coronavirus (SADS-CoV) causes severe enteric disease in neonatal piglets, resulting in substantial economic losses worldwide.
Haixin Huang +14 more
doaj +2 more sources
SARS-CoV-2 Nsp5 Activates NF-κB Pathway by Upregulating SUMOylation of MAVS
Frontiers in Immunology, 2021 The COVID-19 is an infectious disease caused by SARS-CoV-2 infection. A large number of clinical studies found high-level expression of pro-inflammatory cytokines in patients infected with SARS-CoV-2, which fuels the rapid development of the disease ...
Qian Peng, Jialu Qiao, Binlian Sun
exaly +3 more sources
Phosphorylation tunes strain-specific protein condensation during rotavirus replication organelle assembly [PDF]
The EMBO JournalIn many viruses, intrinsically disordered proteins (IDPs) drive the formation of replicative organelles via liquid–liquid phase separation (LLPS). In species A rotaviruses, the disordered protein NSP5 forms condensates with NSP2, but its high sequence ...
Julia Acker +19 more
doaj +2 more sources
Microbiology Spectrum, 2022 Over the last 2 years, several global virus-host interactome studies have been published with SARS-CoV-2 proteins with the purpose of better understanding how specific viral proteins can subvert or utilize different cellular processes to promote viral ...
Zsolt Toth, Zhe Ma
exaly +3 more sources
Ferritinophagy Rewires Carnitine-Dependent Lipid Metabolism to Inhibit PRRSV and IAV Replication. [PDF]
Adv Sci (Weinh)NCOA4‐mediated ferritinophagy reprograms carnitine metabolism by disrupting Fe‐S cluster biogenesis, thereby establishing an iron‐lipid axis that suppresses various viruses, including PRRSV and IAV. However, viruses counteract this mechanism by degrading NCOA4.
Guan K +7 more
europepmc +2 more sources
PDCoV NSP5 cleaves the selective autophagy receptor CCDC50 to disrupt autophagic degradation of the viral envelope protein [PDF]
mBioSelective autophagy is a critical host defense mechanism that eliminates viral components through lysosomal degradation during coronavirus infection. Coronaviruses (CoVs), however, deploy countermeasures that disrupt this process, and several underlying ...
Ke Li +11 more
doaj +2 more sources
Two Lineages of DS-1-Like G3P[8] Human Rotaviruses in Sabah, Malaysia: Genetic Diversity and Evidence of Reassortment. [PDF]
J Med VirolABSTRACT Group A rotavirus (RVA) genotype G3P[8] strains typically carry a Wa‐like genomic backbone, but DS‐1‐like G3P[8] reassortants have disseminated globally since 2013. In Sabah, Malaysia, surveillance in 2018–2019 identified frequent G3P[8] strains with a short RNA electropherotype, consistent with DS‐1‐like backbones.
Akari Y +11 more
europepmc +2 more sources
Rotavirus-NSP5 interacts with and hijacks host ATP citrate lyase to viroplasms, enhancing lipid biosynthesis for facilitating rotavirus infection [PDF]
VirulenceRotavirus (RV) replication occurs within viroplasms (VMs) and is initiated by two RV non-structural proteins NSP2 and NSP5. Viruses exploit host cellular components for their replication and assembly; however, information on the roles of host proteins in
Ranjana Sharma +7 more
doaj +2 more sources
PRRSV NSP5 orchestrates dual immune disruption by targeting NLRP3 and STING [PDF]
Veterinary ResearchInflammasomes and interferons are two critical defense mechanisms of innate immunity, and their imbalance is a key strategy employed by viruses to evade host immune surveillance.
Xiangyu Huang +8 more
doaj +2 more sources