Results 31 to 40 of about 5,845 (181)

Interaction of Rotavirus Polymerase VP1 with Nonstructural Protein NSP5 Is Stronger than That with NSP2 [PDF]

Journal of Virology, 2007
ABSTRACT Rotavirus morphogenesis starts in intracellular inclusion bodies called viroplasms. RNA replication and packaging are mediated by several viral proteins, of which VP1, the RNA-dependent RNA polymerase, and VP2, the core scaffolding protein, were shown to be sufficient to provide replicase activity in vitro.
ARNOLDI, Francesca, M. Campagna, C. Eichwald, U. Desselberger, O. R. Burrone   +4 more
openaire   +5 more sources

Amino acid T25 in the substrate-binding domain of SARS-CoV-2 nsp5 is involved in viral replication in the mouse lung. [PDF]

PLoS ONE
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 5 (nsp5) is a cysteine protease involved in viral replication and suppression of the host immune system.
Yoshiro Sugiura, Kenta Shimizu, Tatsuki Takahashi, Shiori Ueno, Haruka Tanigou, Sodbayasgalan Amarbayasgalan, Wataru Kamitani   +6 more
doaj   +2 more sources

Porcine reproductive and respiratory syndrome virus NSP5 exploited UBE2L6 to promote viral replication via antagonising host RLRs and ISGylation [PDF]

Veterinary Research
Porcine reproductive and respiratory syndrome virus (PRRSV) inhibits the host innate immune response to promote its replication. The ubiquitin–proteasome system (UPS) and ISGylation both play roles in modulating host innate immunity. Within this process,
Zhenbang Zhu, Lulu Chen, Meng Zhang, Qianwen Lin, Yifan Yan, Wenqiang Wang, Wei Wen, Zhendong Zhang, Xiangdong Li   +8 more
doaj   +2 more sources

Porcine reproductive and respiratory syndrome virus nsp5 inhibits the activation of the Nrf2/HO-1 pathway by targeting p62 to antagonize its antiviral activity [PDF]

Journal of Virology
Porcine reproductive and respiratory syndrome virus (PRRSV) infections often trigger oxidative stress and cytokine storms, resulting in significant tissue damage that causes fatalities in piglets and reproductive issues in sows.
Fang Wang, Fructueux Modeste Amona, Yipeng Pang, Qiaoya Zhang, Yuan Liang, Xiaohan Chen, Yongding Ke, Junhao Chen, Chengchuang Song, Yanhong Wang, Zongyun Li, Chunlei Zhang, Xingtang Fang, Xi Chen   +13 more
doaj   +2 more sources

Broad antagonism of coronaviruses nsp5 to evade the host antiviral responses by cleaving POLDIP3.

PLoS Pathogens, 2023
Coronaviruses (CoVs) are a family of the largest RNA viruses that typically cause respiratory, enteric, and hepatic diseases in animals and humans, imposing great threats to the public safety and animal health.
Yang Wu, Mingwei Li, Jin Tian, Haoxin Yan, Yudi Pan, Hongyan Shi, Da Shi, Jianfei Chen, Longjun Guo, Li Feng   +9 more
doaj   +3 more sources

Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus

Journal of Virological Methods, 2017
Infectious bronchitis virus (IBV) continues to be one of the most important poultry pathogens worldwide. The current commercially available enzyme-linked immunosorbent assay (ELISA) kits for IBV specific antibody detection are mostly based on the whole virion, and few serological tests based on nonstructural proteins of IBV have been developed. Herein,
Tingting Shi, Yulan Jin, Min Liao
exaly   +3 more sources

Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease. [PDF]

Sci Adv
E166V in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nsp5 protease confers strong resistance to the antiviral component of Paxlovid, nirmatrelvir (NIR), in passaging and clinical samples. In SARS-CoV-2 replicons, E166V drastically decreased Washington (WA1) but not Omicron (BA.1) fitness (20- versus 2-fold), suggesting
Neilsen G, Lan S, Slack RL, Lorson ZC, Emanuelli Castaner A, Lee R, Edwards KG, Zhang H, Lee J, Cantara WA, Cilento ME, Zhang H, De R, Amblard F, Tedbury PR, Kirby KA, Schinazi RF, Sarafianos SG.   +17 more
europepmc   +3 more sources

Effects of intrabodies specific for rotavirus NSP5 during the virus replicative cycle

Journal of General Virology, 2004
Intracellular antibodies or intrabodies (ICAbs) have great potential in protein knockout strategies for intracellular antigens. In this study, they have been used to investigate the role of the rotavirus non-structural protein NSP5 in the virus replication cycle. Intracellular antibody-capture technology was used to select single-chain Fv format (scFv)
VASCOTTO F, CAMPAGNA M, VISINTIN M, CATTANEO, ANTONINO, BURRONE OR   +4 more
openaire   +5 more sources

Structure Function Studies of Rotavirus NSP5 [PDF]

, 2008
Rotaviruses, causative agents of gastroenteritis in young animals and humans, are large icosahedral viruses with a complex architecture. The double-stranded RNA (dsRNA) genome composed of 11 segments, that codes for 6 structural and 6 non-structural proteins, is enclosed within three concentric capsid layers.
Muszynski, Bartosz
openaire   +2 more sources

Porcine deltacoronavirus nsp5 inhibits interferon-β production through the cleavage of NEMO

Virology, 2017
Porcine deltacoronavirus (PDCoV) causes acute enteric disease and mortality in seronegative neonatal piglets. Previously we have demonstrated that PDCoV infection suppresses the production of interferon-beta (IFN-β), while the detailed mechanisms are poorly understood.
Liurong Fang, Dang Wang, Jiyao Chen
exaly   +3 more sources