Results 31 to 40 of about 9,079 (162)

Functional Analysis of nsP3 Phosphoprotein Mutants ofSindbisVirus [PDF]

Journal of Virology, 2003
ABSTRACT Alphavirus nsP3 phosphoprotein is essential for virus replication and functions initially within polyprotein P123 or P23 components of the short-lived minus-strand replicase, and upon polyprotein cleavage, mature nsP3 likely functions also in plus-strand synthesis.
Indra, Dé, Cori, Fata-Hartley, Stanley G, Sawicki, Dorothea L, Sawicki   +3 more
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The nsp3 Macrodomain Promotes Virulence in Mice with Coronavirus-Induced Encephalitis [PDF]

Journal of Virology, 2015
ABSTRACT All coronaviruses encode a macrodomain containing ADP-ribose-1″-phosphatase (ADRP) activity within the N terminus of nonstructural protein 3 (nsp3). Previous work showed that mouse hepatitis virus strain A59 (MHV-A59) with a mutated catalytic site (N1348A) replicated similarly to wild-type virus but was unable to cause ...
Anthony R, Fehr, Jeremiah, Athmer, Rudragouda, Channappanavar, Judith M, Phillips, David K, Meyerholz, Stanley, Perlman   +5 more
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Crystal structures of SARS-CoV-2 ADP-ribose phosphatase: from the apo form to ligand complexes

IUCrJ, 2020
Among 15 nonstructural proteins (Nsps), the newly emerging Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) encodes a large, multidomain Nsp3. One of its units is the ADP-ribose phosphatase domain (ADRP; also known as the macrodomain, MacroD),
Karolina Michalska, Youngchang Kim, Robert Jedrzejczak, Natalia I. Maltseva, Lucy Stols, Michael Endres, Andrzej Joachimiak   +6 more
doaj   +1 more source

Recognition of eIF4G by Rotavirus NSP3 Reveals a Basis for mRNA Circularization [PDF]

Molecular Cell, 2002
Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G.
Groft, Caroline M., Burley, Stephen K.
openaire   +2 more sources

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies.

PLoS ONE, 2021
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize.
Lee A Armstrong, Sven M Lange, Virginia Dee Cesare, Stephen P Matthews, Raja Sekhar Nirujogi, Isobel Cole, Anthony Hope, Fraser Cunningham, Rachel Toth, Rukmini Mukherjee, Denisa Bojkova, Franz Gruber, David Gray, Paul G Wyatt, Jindrich Cinatl, Ivan Dikic, Paul Davies, Yogesh Kulathu   +17 more
doaj   +1 more source

Rotavirus Nonstructural Protein NSP3 Is Not Required for Viral Protein Synthesis [PDF]

Journal of Virology, 2006
ABSTRACT Initiation is the rate-limiting step in protein synthesis and therefore an important target for regulation. For the initiation of translation of most cellular mRNAs, the cap structure at the 5′ end is bound by the translation factor eukaryotic initiation factor 4E (eIF4E), while the poly(A) tail, at the 3′ end, is recognized by the ...
Hilda, Montero, Carlos F, Arias, Susana, Lopez   +2 more
openaire   +2 more sources

Structure of the X (ADRP) domain of nsp3 from feline coronavirus [PDF]

Acta Crystallographica Section D Biological Crystallography, 2009
The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site.
Wojdyla, J. A., Manolaridis, I., Snijder, E. J., Gorbalenya, A. E., Coutard, B., Piotrowski, Y., Hilgenfeld, R., Tucker, P. A.   +7 more
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Crystal structure of the CoV-Y domain of SARS-CoV-2 nonstructural protein 3

Scientific Reports, 2023
Replication of the coronavirus genome starts with the formation of viral RNA-containing double-membrane vesicles (DMV) following viral entry into the host cell.
Yunfeng Li, Yulia Pustovalova, Wuxian Shi, Oksana Gorbatyuk, Sridhar Sreeramulu, Harald Schwalbe, Jeffrey C. Hoch, Bing Hao   +7 more
doaj   +1 more source

Recognition of the Rotavirus mRNA 3′ Consensus by an Asymmetric NSP3 Homodimer [PDF]

Cell, 2002
Rotaviruses, the cause of life-threatening diarrhea in humans and cattle, utilize a functional homolog of poly(A) binding protein (PABP) known as nonstructural protein 3 (NSP3) for translation of viral mRNAs. NSP3 binds to viral mRNA 3' consensus sequences and circularizes the mRNA via interactions with eIF4G.
Deo, Rahul C., Groft, Caroline M., Rajashankar, K.R., Burley, Stephen K.   +3 more
openaire   +2 more sources

Combined structural, biochemical and cellular evidence demonstrates that both FGDF motifs in alphavirus nsP3 are required for efficient replication [PDF]

Open Biology, 2016
Recent findings have highlighted the role of the Old World alphavirus non-structural protein 3 (nsP3) as a host defence modulator that functions by disrupting stress granules, subcellular phase-dense RNA/protein structures formed upon environmental ...
Tim Schulte, Lifeng Liu, Marc D. Panas, Bastian Thaa, Nicole Dickson, Benjamin Götte, Adnane Achour, Gerald M. McInerney   +7 more
doaj   +1 more source