Results 31 to 40 of about 14,170 (209)

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies.

PLoS ONE, 2021
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize.
Lee A Armstrong, Sven M Lange, Virginia Dee Cesare, Stephen P Matthews, Raja Sekhar Nirujogi, Isobel Cole, Anthony Hope, Fraser Cunningham, Rachel Toth, Rukmini Mukherjee, Denisa Bojkova, Franz Gruber, David Gray, Paul G Wyatt, Jindrich Cinatl, Ivan Dikic, Paul Davies, Yogesh Kulathu   +17 more
doaj   +1 more source

Prediction of Anti-SARS CoV-2 Activity from Green Tea Catechin (Camellia sinensis L. Kuntze) Compound Against To Receptors Non-structural Protein 3 (6W6Y) And Non-structural Protein 5 (6M2N)

Majalah Obat Tradisional, 2022
Green tea catechin compounds (Camellia sinensis L. Kuntze) have an antiviral activity such as influenza, hepatitis B, hepatitis C, herpes simplex virus, HIV, and proven in vitro antiviral influenza against NSP5 in SARS CoV.
Roihatul Mutiah, Chamlah Ayatillah, Yen yen Ari Indrawijaya, Arief Suryadinata   +3 more
doaj   +1 more source

Scalable Inhibitors of the Nsp3–Nsp4 Coupling in SARS-CoV-2

ACS Omega, 2023
The human Betacoronavirus SARS-CoV-2 is a novel pathogen claiming millions of lives and causing a global pandemic that has disrupted international healthcare systems, economies, and communities. The virus is fast mutating and presenting more infectious but less lethal versions. Currently, some small-molecule therapeutics have received FDA emergency use
Abdul-Rahman Azizogli, Varun Pai, Francesco Coppola, Roya Jafari, Joseph B. Dodd-o, Rohan Harish, Bhavani Balasubramanian, Jatin Kashyap, Amanda M. Acevedo-Jake, Petr Král, Vivek A. Kumar   +10 more
openaire   +2 more sources

Predicted binding interface between coronavirus nsp3 and nsp4 [PDF]

, 2022
AbstractDouble membrane vesicles (DMVs) in coronavirus-infected cells feature pores that span both membranes. DMV pores were observed to have six-fold symmetry and include the nsp3 protein. Co-expression of SARS-CoV nsp3 and nsp4 induces DMV formation, and elements of nsp3 and nsp4 have been identified that are essential for membrane disruption.
openaire   +1 more source

Crystal structure of the CoV-Y domain of SARS-CoV-2 nonstructural protein 3

Scientific Reports, 2023
Replication of the coronavirus genome starts with the formation of viral RNA-containing double-membrane vesicles (DMV) following viral entry into the host cell.
Yunfeng Li, Yulia Pustovalova, Wuxian Shi, Oksana Gorbatyuk, Sridhar Sreeramulu, Harald Schwalbe, Jeffrey C. Hoch, Bing Hao   +7 more
doaj   +1 more source

The nsp3 Macrodomain Promotes Virulence in Mice with Coronavirus-Induced Encephalitis [PDF]

Journal of Virology, 2015
ABSTRACT All coronaviruses encode a macrodomain containing ADP-ribose-1″-phosphatase (ADRP) activity within the N terminus of nonstructural protein 3 (nsp3). Previous work showed that mouse hepatitis virus strain A59 (MHV-A59) with a mutated catalytic site (N1348A) replicated similarly to wild-type virus but was unable to cause ...
Anthony R, Fehr, Jeremiah, Athmer, Rudragouda, Channappanavar, Judith M, Phillips, David K, Meyerholz, Stanley, Perlman   +5 more
openaire   +2 more sources

Sequestration of G3BP coupled with efficient translation inhibits stress granules in Semliki Forest virus infection [PDF]

, 2012
Dynamic, mRNA-containing stress granules (SGs) form in the cytoplasm of cells under environmental stresses, including viral infection. Many viruses appear to employ mechanisms to disrupt the formation of SGs on their mRNAs, suggesting that they represent
Eng, Kai Er, Karlsson Hedestam, Gunilla B., Lulla, Aleksei, McInerney, Gerald M., Merits, Andres, Panas, Marc D., Varjak, Margus   +6 more
core   +4 more sources

Rotavirus Nonstructural Protein NSP3 Is Not Required for Viral Protein Synthesis [PDF]

Journal of Virology, 2006
ABSTRACT Initiation is the rate-limiting step in protein synthesis and therefore an important target for regulation. For the initiation of translation of most cellular mRNAs, the cap structure at the 5′ end is bound by the translation factor eukaryotic initiation factor 4E (eIF4E), while the poly(A) tail, at the 3′ end, is recognized by the ...
Hilda, Montero, Carlos F, Arias, Susana, Lopez   +2 more
openaire   +2 more sources

ZAP's stress granule localization is correlated with its antiviral activity and induced by virus replication. [PDF]

, 2019
Cellular antiviral programs encode molecules capable of targeting multiple steps in the virus lifecycle. Zinc-finger antiviral protein (ZAP) is a central and general regulator of antiviral activity that targets pathogen mRNA stability and translation ...
Jurado, Andrea, Law, Lok Man John, Li, Melody MH, MacDonald, Margaret R, Razooky, Brandon S, Rice, Charles M, You, Shihyun   +6 more
core   +2 more sources

Rotavirus Protein NSP3 Shuts Off Host Cell Protein Synthesis

Virology, 2002
A recombinant vaccinia virus encoding rotavirus protein NSP3 driven by an internal ribosome entry site (IRES) from the encephalomyocarditis (EMC) virus was able to abate protein synthesis in BSC1 cells by 25-fold, with as much as 30% of the remaining protein synthesis being NSP3.
Padilla-Noriega, Luis, Paniagua, Octavio, Guzmán-León, Simón   +2 more
openaire   +2 more sources