Recurrent Loss of Macrodomain Activity in Host Immunity and Viral Proteins
Pathogens, 2023 Protein post-translational modifications (PTMs) are an important battleground in the evolutionary arms races that are waged between the host innate immune system and viruses.
Andrew P Ryan, Matthew D Daugherty
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Discovery and Development Strategies for SARS-CoV-2 NSP3 Macrodomain Inhibitors
Pathogens, 2023 The worldwide public health and socioeconomic consequences caused by the COVID-19 pandemic highlight the importance of increasing preparedness for viral disease outbreaks by providing rapid disease prevention and treatment strategies.
Marion Schuller +2 more
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Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells [PDF]
Communications BiologyMono-ADP-ribosylation, a modification of both proteins and nucleic acids, is implicated in innate immunity. Intracellularly, this modification is catalyzed by PARP enzymes, some induced in response to interferons.
Sarah Knapp +16 more
doaj +2 more sources
Viruses, 2023 Among the different drug targets of SARS-CoV-2, a multi-domain protein known as NSP3 is a critical element of the translational and replication machinery.
Anwar Mohammad +2 more
exaly +3 more sources
The Mac1 ADP-ribosylhydrolase is a therapeutic target for SARS-CoV-2 [PDF]
eLifeSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to pose a threat to public health. Current therapeutics remain limited to direct-acting antivirals that lack distinct mechanisms of action and are already showing signs of viral ...
Rahul K Suryawanshi +30 more
doaj +2 more sources
Mutations differentially affecting the coronavirus Mac1 ADP-ribose binding and hydrolysis activities indicate that it promotes multiple stages of the viral replication cycle [PDF]
Journal of VirologyAll coronaviruses (CoVs) encode a conserved macrodomain, termed Mac1, in non-structural protein 3 (nsp3) that binds and hydrolyzes ADP-ribose covalently attached to proteins.
Joseph J. O'Connor +9 more
doaj +2 more sources
Family-wide analysis of human macrodomains reveals novel activities and identifies PARG as most efficient ADPr-RNA hydrolase [PDF]
Communications BiologyADP-ribosylation is well-known as protein posttranslational modification and was recently also identified as RNA posttranscriptional modification. When macrodomain proteins were identified as protein ADP-ribosylhydrolases, several ADP-ribosylation ...
Lisa Weixler +9 more
doaj +2 more sources
PARP14 is regulated by the PARP9/DTX3L complex and promotes interferon γ-induced ADP-ribosylation [PDF]
The EMBO JournalProtein ADP-ribosylation plays important but ill-defined roles in antiviral signalling cascades such as the interferon response. Several viruses of clinical interest, including coronaviruses, express hydrolases that reverse ADP-ribosylation catalysed by ...
Victoria Chaves Ribeiro +2 more
doaj +2 more sources
HTRF-based assay for detection of mono-ADP-ribosyl hydrolyzing macrodomains and inhibitor screening [PDF]
iScienceSummary: The COVID-19 pandemic has highlighted the lack of effective, ready-to-use antivirals for the treatment of viruses with pandemic potential. The development of a diverse drug portfolio is therefore crucial for pandemic preparedness.
Niklas Ildefeld +3 more
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Molecular docking study and molecular dynamics simulation of spice metabolites against main protease enzymes and NSP3 macrodomain SARS CoV-2 [PDF]
Pharmaciana, 2022 COVID-19 is still a global pandemic. The transmission is very fast and wide. Its prevalence continues to increase. There is no specific antiviral drug for SARS CoV 2 yet.
Purwaniati Purwaniati, Asep Roni
doaj +1 more source