Results 251 to 260 of about 84,952 (272)
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Frontiers in Optics + Laser Science 2023 (FiO, LS), 2023
We report a rapid and automated viral plaque assay using time-lapse holographic imaging and deep learning, significantly reducing the detection time needed for traditional viral plaque assays and entirely eliminating staining and manual counting procedures.
Yuzhu Li +10 more
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We report a rapid and automated viral plaque assay using time-lapse holographic imaging and deep learning, significantly reducing the detection time needed for traditional viral plaque assays and entirely eliminating staining and manual counting procedures.
Yuzhu Li +10 more
openaire +1 more source
Canadian Journal of Microbiology, 1969
A plaque assay and some growth characteristics of Egtved virus in a line of rainbow trout cells (RTG-2) are described. Preliminary experiments with metabolic inhibitors suggest that the viral genome is ribonucleic acid.
J B, Campbell, K, Wolf
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A plaque assay and some growth characteristics of Egtved virus in a line of rainbow trout cells (RTG-2) are described. Preliminary experiments with metabolic inhibitors suggest that the viral genome is ribonucleic acid.
J B, Campbell, K, Wolf
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Infectious Viral Quantification of Chikungunya Virus—Virus Plaque Assay
2016The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via
Parveen, Kaur +2 more
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Archives of Virology, 1984
The widely used tetrazolium dye, MTT, has several advantages as a vital stain in the identification of viral plaques. First, since the yellow colored dye MTT stains live cells dark blue, viral plaques can be counted without removal of the phenol red containing agar overlay. Secondly, the high contrast between live and dead cells afforded by MTT permits
R. J. Klebe, June V. Harriss
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The widely used tetrazolium dye, MTT, has several advantages as a vital stain in the identification of viral plaques. First, since the yellow colored dye MTT stains live cells dark blue, viral plaques can be counted without removal of the phenol red containing agar overlay. Secondly, the high contrast between live and dead cells afforded by MTT permits
R. J. Klebe, June V. Harriss
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Journal of Virological Methods, 1996
Viral susceptibility testing has been shown to have a role in the management of patients with herpes simplex infections. In this study, 25 isolates of herpes simplex virus representing a broad spectrum of acyclovir-susceptible and -resistant phenotypes were tested using a microplate in situ enzyme-linked immunosorbent assay (MISE).
B J, Leahy +2 more
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Viral susceptibility testing has been shown to have a role in the management of patients with herpes simplex infections. In this study, 25 isolates of herpes simplex virus representing a broad spectrum of acyclovir-susceptible and -resistant phenotypes were tested using a microplate in situ enzyme-linked immunosorbent assay (MISE).
B J, Leahy +2 more
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Journal of Aquatic Animal Health, 1991
Abstract Sera collected from cultured rainbow trout Oncorhynchus mykiss surviving outbreaks of infectious hematopoietic necrosis (IHN) or viral hemorrhagic septicemia (VHS) were examined for the presence of antibodies to both of the causative viruses, infectious hematopoietic necrosis virus (IHNV) and Egtved virus (viral hemorrhagic septicemia virus ...
P. E.Vestergaard Jorgensen +4 more
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Abstract Sera collected from cultured rainbow trout Oncorhynchus mykiss surviving outbreaks of infectious hematopoietic necrosis (IHN) or viral hemorrhagic septicemia (VHS) were examined for the presence of antibodies to both of the causative viruses, infectious hematopoietic necrosis virus (IHNV) and Egtved virus (viral hemorrhagic septicemia virus ...
P. E.Vestergaard Jorgensen +4 more
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A simple and highly repeatable viral plaque assay for enterovirus 71.
Journal of basic microbiology, 2016The classic plaque assay is a method for counting infectious viral particles, however its complexity limits its use in a variety of virological experiments. To simplify the operation and to improve the repeatability, we employed an improved plaque assay procedure based on Avicel to make the whole experiment easier and optimize the results on a model of
Yingxian, Yin +4 more
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Archives of virology, 1984
The widely used tetrazolium dye, MTT, has several advantages as a vital stain in the identification of viral plaques. First, since the yellow colored dye MTT stains live cells dark blue, viral plaques can be counted without removal of the phenol red containing agar overlay. Secondly, the high contrast between live and dead cells afforded by MTT permits
R J, Klebe, J V, Harriss
openaire +1 more source
The widely used tetrazolium dye, MTT, has several advantages as a vital stain in the identification of viral plaques. First, since the yellow colored dye MTT stains live cells dark blue, viral plaques can be counted without removal of the phenol red containing agar overlay. Secondly, the high contrast between live and dead cells afforded by MTT permits
R J, Klebe, J V, Harriss
openaire +1 more source
A simple and highly repeatable viral plaque assay for enterovirus 71
Journal of Basic Microbiology, 2014Yingxian Yin +4 more
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Stain-free, rapid, and quantitative viral plaque assay using deep learning and holography.
CoRR, 2022Tairan Liu +8 more
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