Results 151 to 160 of about 344,723 (331)

Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276

FEBS Open Bio, EarlyView.
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig, Swanhild Lohse, Sara Elahi, Nils Hartmann, Stefanie Deckert, Shutian Si, Alexander Desuki, Anja Harder   +7 more
wiley   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Comparative utility of restriction fragment length polymorphism analysis and gene sequencing to the molecular epidemiological investigation of a viral outbreak [PDF]

, 2001
Tony L. Goldberg, R. M. Weigel, Edwin C. Hahn, Gail Scherba   +3 more
openalex   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

TRIM5α: A Protean Architect of Viral Recognition and Innate Immunity

Viruses
The evolutionary pressures exerted by viral infections have led to the development of various cellular proteins with potent antiviral activities, some of which are known as antiviral restriction factors.
Stephanie J. Spada, Michael E. Grigg, Fadila Bouamr, Sonja M. Best, Peijun Zhang   +4 more
doaj   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Temporal associations of the COVID-19 related border restrictions and respiratory viral infections in New Zealand

, 2022
Q. Sue Huang, Nikki Turner, Timothy J. Wood, Jemma L. Geoghegan, Andrew Anglemyer, Peter McIntyre, Joep de Ligt, Nayyereh Aminisani, Lauren Jelley, Sarah Jefferies, Tony Dowell, Adrian Trenholme, Cass Brynes, Michelle Balm, Christine McIntosh, Cameron Grant, Annette Nesdale, Hazel Dobinson, Priscilla Campbell‐Stokes, Karen Daniells, Ruth Seeds, Tineke Jennings, Megan Rensburg, Jort Cueto, Ernest Caballero, Joshma John, Emmanuel Penghulan, Chor Tan, Xiaoyun Ren, Klarysse Berquist, Meaghan O’Neill, Maritza Marull, Chang Yu, Andrea McNeill, Tomasz Kiedrzynski, Sally Roberts, Colin McArthur, Alicia Stanley, Susan Taylor, Conroy Wong, Shirley Lawrence, Michael G. Baker, Amanda Kvalsvig, Koen Van Der Werff, Gary McAuliffe, Hannah Antoszewska, Meik Dilcher, Jennifer Fahey, Anja Werno, Juliet Elvy, Jennifer Grant, Michael Addidle, Nicolas Zacchi, Chris Mansell, Marc‐Alain Widdowson, Paul G. Thomas, NPIsImpactOnFlu Consortium, Richard J. Webby   +57 more
openalex   +1 more source

The Robust Restriction of Zika Virus by Type-I Interferon in A549 Cells Varies by Viral Lineage and Is Not Determined by IFITM3 [PDF]

, 2020
Theodore A. Gobillot, Daryl Humes, Amit Sharma, Caroline Kikawa, Julie Overbaugh   +4 more
openalex   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source