Results 91 to 100 of about 540,107 (353)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics

Frontiers in Veterinary Science
The interaction between viral proteins and host proteins plays a crucial role in the process of virus infecting cells. Tags such as HA, His, and Flag do not interfere with the function of fusion proteins and are commonly used to study protein–protein ...
Ruiwei Wang, Xuhong Cao, Kejia Lu, Zhengwu Chang, Xiaoyu Dong, Hanwei Guo, Xi Wei, Ruyi Dang, Juan Wang, Xinglong Wang, Sa Xiao, Haijin Liu, Zengqi Yang   +12 more
doaj   +1 more source

Antigenic properties of sARs-CoV-2/human/RUs/nsk-FRCFtM-1/2020 coronavirus isolate from a patient in novosibirsk

Журнал инфектологии, 2020
Objective: isolation of coronavirus SARS-CoV-2 from clinical sample of patient with COVID-19 in Novosibirsk; obtaining a purified and inactivated viral antigen and study of its antigenic properties.
A. A. Chepurnov, K. A. Sharshov, E. I. Kazachinskaya, Yu. V. Kononova, E. A. Kazachkova, O. P. Khripko, K. S. Yurchenko, A. Yu. Alekseev, M. I. Voevoda, A. M. Shestopalov   +9 more
doaj   +1 more source

Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]

, 2016
Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart, Anna Janowicz, BMJ Group, Frauke Seehusen, Ilaria M. Piras, Kerstin Hahn, Marco Caporale, Mariana Varela, Massimo Palmarini, Rute Maria Pinto, S. López, William Marciel de Souza, Wolfgang Baumgärtner, Xiaohong Shi   +13 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

The Epstein-Barr Virus Immunoevasins BCRF1 and BPLF1 Are Expressed by a Mechanism Independent of the Canonical Late Pre-initiation Complex.

PLoS Pathogens, 2016
Subversion of host immune surveillance is a crucial step in viral pathogenesis. Epstein-Barr virus (EBV) encodes two immune evasion gene products, BCRF1 (viral IL-10) and BPLF1 (deubiquitinase/deneddylase); both proteins suppress antiviral immune ...
Jessica McKenzie, Francesc Lopez-Giraldez, Henri-Jacques Delecluse, Ann Walsh, Ayman El-Guindy   +4 more
doaj   +1 more source

Hepatitis C virus NS5A targets the nucleosome assembly protein NAP1L1 to control the innate cellular response [PDF]

, 2017
Hepatitis C virus (HCV) is a single-stranded positive-sense RNA hepatotropic virus. Despite cellular defenses, HCV is able to replicate in hepatocytes and to establish a chronic infection that could lead to severe complications and hepatocellular ...
Cesarec, Mia, Da Silva Filipe, Ana, Licastro, Danilo, Marcello, Alessandro, McLauchlan, John, Çevik, Recep Emrah   +5 more
core   +1 more source

Stochastic Kinetics of Viral Capsid Assembly Based on Detailed Protein Structures [PDF]

Biophysical Journal, 2006
We present a generic computational framework for the simulation of viral capsid assembly which is quantitative and specific. Starting from PDB files containing atomic coordinates, the algorithm builds a coarse grained description of protein oligomers based on graph rigidity. These reduced protein descriptions are used in an extended Gillespie algorithm
Hemberg, M, Yaliraki, SN, Barahona, M
openaire   +5 more sources

Super‐Refractory Status Epilepticus (SRSE) in a Patient With Compound Heterozygous OPA1 Variants: Case Report and Literature Review

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Super‐Refractory Status Epilepticus (SRSE) is a rare, life‐threatening neurological emergency with unclear etiology in many cases. Mitochondrial dysfunction, often due to disease‐causing genetic variants, is increasingly recognized as a cause, with each gene producing distinct pathophysiological mechanisms.
Pouria Mohammadi, Lara Basovic, Christopher Michael McGraw   +2 more
wiley   +1 more source

Cellular origin of the viral capsid-like bacterial microcompartments

Biology Direct, 2017
ᅟ Bacterial microcompartments (BMC) are proteinaceous organelles that structurally resemble viral capsids, but encapsulate enzymes that perform various specialized biochemical reactions in the cell cytoplasm.
Mart Krupovic, Eugene V. Koonin
doaj   +1 more source