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Optimization of YF17D-Vectored Zika Vaccine Production by Employing Small-Molecule Viral Sensitizers to Enhance Yields [PDF]

Vaccines
Background: Modern viral vector production needs to consider process intensification for higher yields from smaller production volumes. However, innate antiviral immunity triggered in the producer cell may limit virus replication.
Sven Göbel, Tilia Zinnecker, Ingo Jordan, Volker Sandig, Andrea Vervoort, Jondavid de Jong, Jean-Simon Diallo, Peter Satzer, Manfred Satzer, Kai Dallmeier, Udo Reichl, Yvonne Genzel   +11 more
doaj   +4 more sources

Vero cell upstream bioprocess development for the production of viral vectors and vaccines

Biotechnology Advances, 2020
The Vero cell line is considered the most used continuous cell line for the production of viral vectors and vaccines. Historically, it is the first cell line that was approved by the WHO for the production of human vaccines. Comprehensive experimental data on the production of many viruses using the Vero cell line can be found in the literature ...
Amine Kamen
exaly   +4 more sources

Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate

Pharmaceutics, 2022
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) homotrimeric spike (S) protein is responsible for mediating host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2) receptor, thus being a key viral antigen to target in a ...
Bárbara Fernandes, Rute Castro, Farien Bhoelan, Denzel Bemelman, Ricardo A. Gomes, Júlia Costa, Patrícia Gomes-Alves, Toon Stegmann, Mario Amacker, Paula M. Alves, Sylvain Fleury, António Roldão   +11 more
doaj   +1 more source

Quality by design modelling to support rapid RNA vaccine production against emerging infectious diseases

npj Vaccines, 2021
Rapid-response vaccine production platform technologies, including RNA vaccines, are being developed to combat viral epidemics and pandemics. A key enabler of rapid response is having quality-oriented disease-agnostic manufacturing protocols ready ahead ...
Damien van de Berg, Zoltán Kis, Carl Fredrik Behmer, Karnyart Samnuan, Anna K. Blakney, Cleo Kontoravdi, Robin Shattock, Nilay Shah   +7 more
doaj   +1 more source

Bioprocess development for universal influenza vaccines based on inactivated split chimeric and mosaic hemagglutinin viruses

Frontiers in Bioengineering and Biotechnology, 2023
Seasonal influenza viruses account for 1 billion infections worldwide every year, including 3–5 million cases of severe illness and up to 650,000 deaths.
Eduard Puente-Massaguer, Annika Beyer, Madhumathi Loganathan, Iden Sapse, Juan Manuel Carreño, Juan Manuel Carreño, Goran Bajic, Weina Sun, Peter Palese, Peter Palese, Florian Krammer, Florian Krammer, Florian Krammer   +12 more
doaj   +1 more source

Overview of recent advances in Vero cells genomic characterization and engineering for high‐throughput vaccine manufacturing

Clinical and Translational Discovery, 2022
Background The Vero cell line is the most used continuous cell line for viral vaccine manufacturing with more than 30 years of accumulated experience in the vaccine industry. Nonetheless, virus production yield with Vero cells remains limited. Therefore,
Marie‐Angélique Sène, Yu Xia, Prof. Amine Abdelkader Kamen   +2 more
doaj   +1 more source

Process Development for Newcastle Disease Virus-Vectored Vaccines in Serum-Free Vero Cell Suspension Cultures

Vaccines, 2021
The ongoing COVID-19 pandemic drew global attention to infectious diseases, attracting numerous resources for development of pandemic preparedness plans and vaccine platforms—technologies with robust manufacturing processes that can quickly be pivoted to
Julia Puppin Chaves Fulber, Omar Farnós, Sascha Kiesslich, Zeyu Yang, Shantoshini Dash, Leonardo Susta, Sarah K. Wootton, Amine A. Kamen   +7 more
doaj   +1 more source

Development and Scalable Production of Newcastle Disease Virus-Vectored Vaccines for Human and Veterinary Use

Viruses, 2022
The COVID-19 pandemic has highlighted the need for efficient vaccine platforms that can rapidly be developed and manufactured on a large scale to immunize the population against emerging viruses.
Julia P. C. Fulber, Amine A. Kamen
doaj   +1 more source

Biolayer Interferometry Analysis for a Higher Throughput Quantification of In-Process Samples of a Rotavirus Vaccine

Vaccines, 2022
Rotavirus A infection is a global leading cause of severe acute gastroenteritis associated with life-threatening diarrheal episodes in infants and young children.
Sofia B. Carvalho, Mafalda M. Dias, Jean-Philippe Matheise, Isabelle Knott, Patrícia Gomes-Alves, Paula M. Alves   +5 more
doaj   +1 more source

An Alternative Perfusion Approach for the Intensification of Virus-Like Particle Production in HEK293 Cultures

Frontiers in Bioengineering and Biotechnology, 2020
Virus-like particles (VLPs) have gained interest over the last years as recombinant vaccine formats, as they generate a strong immune response and present storage and distribution advantages compared to conventional vaccines.
Jesús Lavado-García, Laura Cervera, Francesc Gòdia   +2 more
doaj   +1 more source