Results 31 to 40 of about 2,929 (132)

Rapid production of a chimeric antibody-antigen fusion protein based on 2A-peptide cleavage and green fluorescent protein expression in CHO cells [PDF]

, 2019
To enable large-scale antibody production, the creation of a stable, high producer cell line is essential. This process often takes longer than 6 months using standard limited dilution techniques and is very labor intensive.
Cox, Eric, Devriendt, Bert, Van Der Weken, Hans   +2 more
core   +2 more sources

Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope Protein

Biotechnology and Bioengineering, Volume 122, Issue 11, Page 3192-3204, November 2025.
ABSTRACT Despite evidence that they are not functional or infective, retrovirus‐like particles (RVLPs), originating from endogenous proviral sequences in Chinese hamster ovary (CHO) cells, present a safety risk for biotherapeutics manufactured using this cell line due to their resemblance to other mammalian leukemia viruses.
Matthew Stuible, Sergio P. Alpuche‐Lazcano, Christian Gervais, Manon Ouimet, Julie Lippens, Martine Pagé, Audrey Morasse, Anna N. Moraitis, Yves Durocher   +8 more
wiley   +1 more source

Impacto de la biotecnología en la inmunología [PDF]

, 2008
La biotecnología ha conducido al desarrollo de la inmunología en la generación de pruebas diagnósticas, fármacos y vacunas, útiles en la profilaxia, diagnóstico y tratamiento de una variedad de enfermedades infecciosas y autoinmunitarias y el cáncer. Hoy
Gómez Flores, Ricardo Alberto, Rodríguez Padilla, Cristina, Tamez Guerra, Patricia, Tamez Guerra, Reyes Silvestre   +3 more
core  

Impact of Host Cell DNA and Chromatin on Virus‐like Particle Analysis by Light Scattering in Asymmetrical Flow Field‐flow Fractionation

Journal of Separation Science, Volume 48, Issue 11, November 2025.
ABSTRACT The development of virus‐like particle (VLP) production processes is often constrained by the extensive number of analytical methods required for their quantification and characterization, as well as the significant labor demands associated with these techniques.
Johanna Bacher, Leo A. Jakob, Tomas Mesurado, Narges Lali, Alexander Zollner, Alois Jungbauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

Engineering Decavalent, Sperm‐Binding Laminin‐IgG Hybrid Antibodies for Potent Non‐Hormonal Contraception

Advanced Science, Volume 12, Issue 38, October 13, 2025.
This study investigates a highly multivalent mAb platform fusing Laminin‐511 heterotrimerization domains with Fabs and IgG1‐Fc as a non‐hormonal contraceptive. The most potent molecule ‘LamH10’, possessing 10 Fabs, rapidly achieved >99% reduction in progressively motile sperm at just 33µg per dose in sheep, making the laminin‐IgG hybrid platform the ...
Alison Schaefer, Keiichiro Kushiro, Bhawana Shrestha, Yong Zhu, Alysha Panjwani, Lauren Dawson, Haley Flowers, Kathleen L. Vincent, Samuel K. Lai   +8 more
wiley   +1 more source

Advances in Plant Molecular Farming [PDF]

, 2011
Plant molecular farming (PMF) is a new branch of plant biotechnology, where plants are engineered to produce recombinant pharmaceutical and industrial proteins in large quantities.
Leelavathi, S., Obembe, Olawole O., Popoola, J.O, Reddy, S.V.   +3 more
core   +1 more source

Design Strategies for Novel Lipid Nanoparticle for mRNA Vaccine and Therapeutics: Current Understandings and Future Perspectives

MedComm, Volume 6, Issue 10, October 2025.
This review summarizes strategies to optimize lipid nanoparticles (LNPs) for improved targeting, endosomal escape, and safety. It highlights mRNA design, alternative delivery systems, and recent therapeutic advances across infectious diseases, cancer, and drug applications.
Xiaochi Li, Junli Li, Jiazheng Wei, Weixin Du, Cheng Su, Xiaobin Shen, Aihua Zhao, Miao Xu   +7 more
wiley   +1 more source

Pseudotyped Lentiviral Vectors: One Vector, Many Guises [PDF]

, 2017
Viruses have evolved specialized molecular mechanisms to transfer their genome efficiently into host cells. Viruses can be repurposed into viral vectors to achieve controlled gene transfer to desired cells.
Joglekar, Alok V., Sandoval, Salemiz
core  

A recurrent neural network for soft sensor development using CHO stable pools in fed‐batch process for SARS‐CoV‐2 spike protein production as a vaccine antigen

Biotechnology Progress, Volume 41, Issue 5, September/October 2025.
Abstract Fed‐batch recombinant therapeutic protein (RTP) production processes utilizing Chinese Hamster Ovary (CHO) cells can take a long period of time (>10 days). Within this period, not all critical features may be measured routinely, and in fact, some are only measured once the process is terminated, complicating decision making.
Sebastian‐Juan Reyes, Robert Voyer, Yves Durocher, Olivier Henry, Phuong Lan Pham   +4 more
wiley   +1 more source

Systematic Review on the Role of Microfluidic Platforms in Advancing Scalable and Precise Microbial Bioprocessing

Engineering in Life Sciences, Volume 25, Issue 9, September 2025.
ABSTRACT Microbial bioprocessing is a key technology for the production of a wide range of biomolecules, including proteins, enzymes, antibiotics, and other bioactive compounds. In recent years, there has been an increasing interest in using microfluidic platforms for bioprocessing, due to the ability to precisely control and manipulate fluids at the ...
Alperen Alpural, Ilgin Kimiz‐Gebologlu, Mayur Parekh, Esra Imamoglu, Zulfiqur Ali, Ozlem Yesil‐Celiktas   +5 more
wiley   +1 more source