Results 141 to 150 of about 2,747,170 (398)

Virus-free induction of pluripotency and subsequent excision of reprogramming factors [PDF]

, 2009
Reprogramming of somatic cells to pluripotency, thereby creating induced pluripotent stem (iPS) cells, promises to transform regenerative medicine. Most instances of direct reprogramming have been achieved by forced expression of defined factors using ...
Agnieszka Paca, AL Szymczak, B Brueckner, BW Carey, E Yeh, H Niwa, I Chambers, IH Park, J Yu, K Hasegawa, K Hochedlinger, K Kaji, K Okita, K Okita, K Takahashi, K Takahashi, Katherine Norrby, Keisuke Kaji, Knut Woltjen, KW Foster, M Nakagawa, M Stadtfeld, M Wernig, M Wernig, Maria Mileikovsky, N Maherali, Paria Mohseni, QL Ying, T Aoi, T Kunath, TS Mikkelsen, W Wang   +31 more
core   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Genetic Characterization of Chikungunya Virus in Field-Caught Aedes aegypti Mosquitoes Collected during the Recent Outbreaks in 2019, Thailand

Pathogens, 2019
Chikungunya virus (CHIKV) is a mosquito-borne virus belonging to the genus Alphavirus. The virus is transmitted to humans by the bite of infected female Aedes mosquitoes, primarily Aedes aegypti.
Proawpilart Intayot, Atchara Phumee, Rungfar Boonserm, Sriwatapron Sor-suwan, Rome Buathong, Supaporn Wacharapluesadee, Narisa Brownell, Yong Poovorawan, Padet Siriyasatien   +8 more
doaj   +1 more source

Viral vector-based tools advance knowledge of basal ganglia anatomy and physiology.

Journal of Neurophysiology, 2016
Viral vectors were originally developed to deliver genes into host cells for therapeutic potential. However, viral vector use in neuroscience research has increased because they enhance interpretation of the anatomy and physiology of brain circuits ...
R. J. Sizemore, Sonja Seeger-Armbruster, S. Hughes, L. Parr-Brownlie   +3 more
semanticscholar   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Viral vectors for malaria vaccine development

Vaccine, 2007
A workshop on viral vectors for malaria vaccine development, organized by the PATH Malaria Vaccine Initiative, was held in Bethesda, MD on October 20, 2005. Recent advancements in viral-vectored malaria vaccine development and emerging vector technologies were presented and discussed.
Li, Shengqiang, Locke, Emily, Bruder, Joseph, Clarke, David, Doolan, Denise L., Havenga, Menzo J. E., Hill, Adrian V. S., Liljestrom, Peter, Monath, Thomas P., Naimi, Hussein Y., Ockenhouse, Christian, Tang, De-chu C., Van Kampen, Kent R., Viret, Jean-Francois, Zavala, Fidel, Dubovsky, Filip   +15 more
openaire   +4 more sources

Experimental evaluation of sand fly collection and storage methods for the isolation and molecular detection of Phlebotomus-borne viruses

Parasites & Vectors, 2015
Background Several viruses have been recently isolated from Mediterranean phlebotomine sand flies; some are known to cause human disease while some are new to science.
Maria Elena Remoli, Gioia Bongiorno, Claudia Fortuna, Antonella Marchi, Riccardo Bianchi, Cristina Khoury, Maria Grazia Ciufolini, Marina Gramiccia   +7 more
doaj   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

Experimental studies on comparison of the vector competence of four Italian Culex pipiens populations for West Nile virus

Parasites & Vectors, 2015
Background West Nile virus (WNV) is a vector-borne disease responsible for causing epidemics in many areas of the world. The virus is maintained in nature by an enzootic bird-mosquito-bird cycle and occasionally transmitted to other hosts, such as ...
Claudia Fortuna, Maria Elena Remoli, Marco Di Luca, Francesco Severini, Luciano Toma, Eleonora Benedetti, Paola Bucci, Fabrizio Montarsi, Giada Minelli, Daniela Boccolini, Roberto Romi, Maria Grazia Ciufolini   +11 more
doaj   +1 more source

Minimal hepatic toxicity of Onyx-015: spatial restriction of coxsackie-adenoviral receptor in normal liver. [PDF]

, 2007
We administered an adenoviral vector, Onyx-015, into the hepatic artery of patients with metastatic colorectal cancer involving the liver. Thirty-five patients enrolled in this multi-institutional phase I/II trial received up to eight arterial infusions ...
Au, T, Kirn, D, Korn, WM, Reid, TR, Sze, D, Thorne, S   +5 more
core