Results 41 to 50 of about 102,321 (242)

Ultrasound‐Actuated Gene Editing in Human Kidney Organoids

Advanced Science, EarlyView.
Peptide‐stabilized nanoemulsions enable spatially controlled, ultrasound‐triggered delivery of gene‐editing proteins deep within human kidney organoid tissues. Focused acoustic pulses drive droplet vaporization, propelling cargo into target renal cells while preserving native tissue microarchitecture. This non‐viral platform improves both the depth and
Michael A. Miller, Nicole Vo, Utkarsh, Ivan Sokirniy, Justin Pritchard, Benjamin S. Freedman, Scott H. Medina   +6 more
wiley   +1 more source

Centrifugation-Based Purification Protocol Optimization Enhances Structural Preservation of Nucleopolyhedrovirus Budded Virion Envelopes

Insects
The structural integrity of viral envelopes is a critical determinant of infectivity for enveloped viruses, directly influencing vector stability, functional accuracy of surface-displayed epitopes, and preservation of native conformational states ...
Yong Pan, Jiming Yan, Yinong Zhang, Jiasheng Lin, Zhiquan Liang, Jingchen Sun   +5 more
doaj   +1 more source

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection [PDF]

Open Biology, 2017
Successful generation of virions from infected cells is a complex process requiring orchestrated regulation of host and viral genes. Cells infected with human cytomegalovirus (HCMV) undergo a dramatic reorganization of membrane organelles resulting in ...
Jonathan Pavelin, Dominique McCormick, Stephen Chiweshe, Saranya Ramachandran, Yao-Tang Lin, Finn Grey   +5 more
doaj   +1 more source

TOLLIP Inhibits Psoriasis Progression via Suppressing PKM2‐Mediated Glycolysis in Keratinocytes

Advanced Science, EarlyView.
In this study, we identify TOLLIP as a critical regulator of psoriasis pathogenesis through its modulation of glycolytic metabolism. Our findings establish the TOLLIP‐PKM2‐glycolysis axis as a key mechanism linking metabolic reprogramming to psoriasis pathogenesis, and propose TOLLIP as a promising therapeutic target.
Xiuhuan Jiang, Guoying Miao, Na Shen, Qingxia Han, Xiaoru Han, Zichang Qiao, Kexin Feng, Zhuo Tan, Yaguang Wang, Xiuhua Liu, Chen Wu, Zhenzhen Yan   +11 more
wiley   +1 more source

THE VIRUS RANGE OF HOP PLANTS: IDENTIFICATION KEY

Екологія, людина, суспільство
The paper presents an identification key for the viruses infecting Humulus lupulus L. based on size and shape of virions. This identification key has coverage of 16 virus species and divides the virus range of H. lupulus into three groups.
Назарій СУС
doaj   +1 more source

Structure and Assembly of the Virion

Intervirology, 1996
This article briefly catalogues most of the recognized virion proteins of cytomegalovirus (CMV), summarizes general features of the virion assembly pathway, and outlines some early protein-protein interactions that appear to play key roles in initiating and guiding the capsid assembly process.
openaire   +2 more sources

Purification of Herpesvirus Virions and Capsids [PDF]

BIO-PROTOCOL, 2014
This protocol was designed for large-scale purification of herpesvirus particles by cell culture. Virions and capsids are isolated from extracellular culture media and cell nuclei, respectively. Purity and concentration of the purified samples are usually sufficient for structural studies with cryo electron microscopy and cryo electron tomography.
Dai, Xinghong, Zhou, Z Hong
openaire   +5 more sources

Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF‐κB Signaling

Advanced Science, EarlyView.
The cysteine redox state of the Hendra virus W (WHeV) protein controls assembly into fibrils or amorphous aggregates, with residues 2–29 required for fibrillation. In cells, WHeV forms different types of nuclear condensates. Impaired ability to form redox‐sensitive, non‐filamentous condensates reduces WHeV inhibition of the NF‐κB pathway, while it ...
Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre‐Yves Lozach, Denis Gerlier, Cyrille Mathieu, Sonia Longhi   +12 more
wiley   +1 more source

VRON-0200 alone and in combination with investigational antivirals for participants with chronic hepatitis B virus infection receiving stable nucleos(t)ide therapy: a phase 1b, randomised, open-label, multicentre trial

The Lancet Microbe
Summary: Background: Functional cure treatments for hepatitis B virus (HBV) have been restricted by their inability to restore anti-HBV immunity, often leading to viral rebound after treatment.
ProfMD Edward Gane, ProfMD Grace Lai-Hung Wong, Sue Currie, PhD, Andrew Luber, PharmD, Paula MacDonald, MSc, Marie Bonhomme, PhD, Tien huey Lim, MD   +6 more
doaj   +1 more source

Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft

Viruses, 2016
The influenza glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are associated with the lipid raft, have the potential to initiate virion budding. However, the role of these viral proteins in infectious virion assembly is still unclear.
Naoki Takizawa, Fumitaka Momose, Yuko Morikawa, Akio Nomoto   +3 more
doaj   +1 more source