Results 31 to 40 of about 246,749 (219)

Chimera X Interface to Enhance Understanding in Biochemistry and Immunology

Biochemistry and Molecular Biology Education, EarlyView.
ABSTRACT Proteins are essential in biological systems, acting in transport, catalysis, and immune defense processes. However, these biomolecules' structural and functional complexity makes teaching and understanding these topics challenging. To address this difficulty, this study aimed to develop tutorials that facilitate learning and teaching about ...
Dalpiaz Giovana, Krohn Muriel Schiling, Groehs Eduarda, Anjos André da Silva, Meireles R. Mariana   +4 more
wiley   +1 more source

A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy

Biotechnology Progress, EarlyView.
Using online metabolite data from the first few days of a bioreactor run, the digital shadow can forecast the cell concentration in real‐time with an accuracy of 13% (standard deviation ≈ 6%). These predictions can guide the optimal harvest time of CAR T cells for subsequent re‐infusion into the patient.
Joseph R. Egan, Núria Marí‐Buyé, Elia Vallejo Benítez‐Cano, Miquel Costa, Linda Wanika, Michael J. Chappell, Ursula Schultz, Jelena Ochs, Manuel Effenberger, David Horna, Qasim Rafiq, Stephen Goldrick   +11 more
wiley   +1 more source

Impact of bioreactor process parameters and yeast biomass on Raman spectra

Biotechnology Progress, EarlyView.
Abstract In‐line Raman spectroscopy combined with chemometric modeling is a valuable process analytical technology (PAT) providing real‐time quantitative information on cell culture compounds. Considering that compound quantification through chemometric models depends on pre‐processing to maintain consistent changes in intensity at certain wavenumbers,
Maarten Klaverdijk, Mehrab Nemati, Marcel Ottens, Marieke E. Klijn   +3 more
wiley   +1 more source

Accelerating AAV purification process development using high‐throughput resin tip module

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated viruses (AAVs) with precise genome editing and cell‐virus interaction have become a promising delivery tool for gene therapy. A robust AAV purification process is crucial for ensuring therapeutic efficacy. The challenges of AAV purification process development encompass limited material availability during early ...
Qingxuan Li, Mahsa Hadidi, Steven Benner, Junfen Ma   +3 more
wiley   +1 more source

PEGylation of polymerized albumin retains colloid osmotic pressure: Towards an enhanced potential plasma substitute

Biotechnology Progress, EarlyView.
Abstract Plasma expanders (PEs) are commonly used to replace lost blood volume for septic shock patients with increased vascular permeability. Human serum albumin (HSA) is the preferred PE, due to its innate ability to restore blood colloid osmotic pressure (COP).
Amna Abdalbaqi, Ahmad Yahya, Krianthan Govender, Carlos Muñoz, Gala Sanchez Van Moer, Daniela Lucas, Pedro Cabrales, Andre F. Palmer   +7 more
wiley   +1 more source

Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression

Biotechnology Progress, EarlyView.
Abstract Industrial fermentation continually improves biological process control for a wide range of microorganisms used in multi‐billion‐dollar industries including industrial enzymes, pharmaceuticals, foods, beverages, commodity chemicals, and bioenergy.
Jennifer Reid, Andrew Szto, Airong Chen, Patricia Gomes, Craig Kearse, Joyce Ni, Tao Yuan   +6 more
wiley   +1 more source

Production of recombinant methionine‐containing elastin‐like polypeptides in a fermenter using ECPM1 medium

Biotechnology Progress, EarlyView.
Abstract Elastin‐like polypeptides (ELPs) are recombinant protein‐like polymers whose macromolecular structure can be precisely controlled through genetic manipulation of their sequence and length. Their lower critical solution temperature (LCST) phase behavior facilitates purification via chromatography‐free techniques and can be explored for self ...
Alice Delhaes, Laure Bataille, Myriam Médéric, Sébastien Lecommandoux, Elisabeth Garanger   +4 more
wiley   +1 more source

Harvesting AAV by tangential flow filtration using reverse asymmetric membranes

Biotechnology Progress, EarlyView.
Abstract Efficient bioreactor clarification for harvesting virus particles is often challenging. Tangential flow filtration is attractive as it can be easily adapted for batch and perfusion operations. Here the feasibility of using reverse asymmetric hollow fiber membranes, where the more open support structure faces the feed stream, has been ...
Xiaolei Hao, Ronny Horax, Xianghong Qian, April Wheeler, Hironobu Shirataki, S. Ranil Wickramasinghe   +5 more
wiley   +1 more source

Innovations in Dental Implants Integration: Optimizing dental implants performance utilizing stem cells and coatings

Biotechnology Progress, EarlyView.
Abstract The last two decades, between 2000 and 2024, significant steps were achieved regarding the interaction between various stem cells and titanium implant surfaces to improve dental implant integration. This literature review focuses on the potential effects of (i) bone marrow mesenchymal stem cells (BMSCs), (ii) periodontal ligament stem cells ...
Ioannis Tsamesidis, Athanasios Christodoulou, Evangelia Stalika, Georgia K. Pouroutzidou, Eleana Kontonasaki   +4 more
wiley   +1 more source

Enhancing rapid Protein A performance in monoclonal antibody processing: Anion exchange chromatographic clarification

Biotechnology Progress, EarlyView.
Abstract Clarification fidelity, including reduction of insoluble and soluble contaminants, has been demonstrated to significantly affect the performance and robustness of the Protein A capture chromatography step during the purification of monoclonal antibodies (mAb) and their derivatives expressed in CHO cell cultures.
Andrew Vail, David Chau, Jennifer Heitkamp, Alexei Voloshin   +3 more
wiley   +1 more source