Results 131 to 140 of about 3,645,587 (361)

Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

BMC Plant Biology, 2010
Background Reference genes are commonly used as the endogenous normalisation measure for the relative quantification of target genes. The appropriate application of quantitative real-time PCR (RT-qPCR), however, requires the use of reference genes whose ...
Kundu Jiban K, Jarošová Jana
doaj   +1 more source

Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]

, 2019
BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak, A Bak, Aurélie Bak, AYT Wong, BM Maghari, C Wolf, E Kamau, G Cottenet, G Cottenet, HD VanGuilder, IB Salisu, IMJ Scholtens, J Davison, JB Emerson, Joanne B. Emerson, K Arumuganathan, K Nybo, K Ramessar, L Garibyan, M Chaouachi, M Singh, M Tajadini, MA Fraiture, MJ Holden, MJ Oliver, Q Zhang, R Becker, RJ Pryor, S Broeders, S Fernandez, S Llewellyn, SA Bustin, T Demeke, W Fu, Y Hou, Y Wu   +35 more
core   +1 more source

Rift Valley Fever Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2019
AbstractRift Valley fever virus (RVFV) is an arthropod‐borne, zoonotic disease endemic to sub‐Saharan Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever have had up to 100% mortality rates in fetal and neonatal sheep. Upon infection of ruminant and human hosts alike, RVFV infection causes an at times severe hepatitis and pathology in many
William C. Wilson, Erin E. Schirtzinger, A. Sally Davis, MaRyka R. Smith   +3 more
openaire   +4 more sources

Serotonergic and Dopaminergic Function in Neuropsychiatrically Asymptomatic People With HIV on Antiretroviral Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective People with HIV (PWH) on antiretroviral therapy (ART) still experience neurocognitive dysfunction and accelerated brain volume loss. To assess whether the serotonergic and dopaminergic systems are affected, we used [11C]DASB positron emission tomography (PET) to assess presynaptic serotonergic function and [18F]FDOPA PET to measure ...
Chuen‐Yen Lau, Anna Lyndaker, Swati Shah, Paul Wakim, Amelia Mitchell, Cynthia Monroe, Alan Kim, Tracy L. Cropper, Govind Nair, Joseph Snow, Bryan R. Smith, Avindra Nath, Dima A. Hammoud   +12 more
wiley   +1 more source

Protocol for the assessment of viral retention capability of membranes [PDF]

, 2011
A series of experiments has been carried out to determine the Log removal value (LRV) of MS2 bacteriophages suspended in various buffers (osmosed water, tap water, aqueous solutions of NaCl and phosphate buffer solution) during filtration through hollow ...
Aimar, Pierre, Bergé, Mathieu, Causserand, Christel, Furiga, Aurélie, Pierre, Gwenaelle, Roques, Christine   +5 more
core   +1 more source

SARS‐CoV‐2 Is Linked to Brain Volume Loss in Multiple Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The impact of SARS‐CoV‐2 infection on brain and spinal cord pathology in patients with multiple sclerosis (pwMS) remains unclear. We aimed to describe changes in brain lesion activity and brain and spinal cord volumes following SARS‐CoV‐2 infection.
Tomas Uher, Dominika Stastna, Ingrid Menkyova, Vaclav Capek, Jiri Lindner, Petra Nytrova, Jan Krasensky, Eliza Varju, Miguel D'haeseleer, Eva Kubala Havrdova, Dana Horakova, Manuela Vaneckova, Niels Bergsland   +12 more
wiley   +1 more source

Digital microarrays: single-molecule readout with interferometric detection of plasmonic nanorod labels [PDF]

, 2018
DNA and protein microarrays are a high-throughput technology that allow the simultaneous quantification of tens of thousands of different biomolecular species.
Daaboul, George G., Kanik, Fulya Ekiz, Sevenler, Derin, Unlu, M. Selim, Unlu, Nese Lortlar   +4 more
core   +1 more source

Plasma microRNA Signature as Predictive Marker of Clinical Response to Therapy During Multiple Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Despite the availability of effective therapies for Multiple Sclerosis (MS), the unpredictable nature of disease progression and the variability in individual treatment outcomes call for reliable biomarkers. This pilot study aims to investigate the potential of plasma circulating microRNAs (miRNAs) as predictive biomarkers for ...
Fortunata Carbone, Alessandra Colamatteo, Teresa Micillo, Gianmarco Abbadessa, Silvia Garavelli, Clorinda Fusco, Claudia Russo, Francesco Perna, Federica Garziano, Claudia La Rocca, Maria Mottola, Giorgia Teresa Maniscalco, Simona Bonavita, Antonio Luca Spiezia, Alessia Castiello, Roberta Lanzillo, Vincenzo Brescia Morra, Claudio Procaccini, Paola de Candia, Giuseppe Matarese   +19 more
wiley   +1 more source

Absolute quantification of viral proteins from pseudotyped VSV-GP using UPLC-MRM

Microbiology Spectrum
The rapidly developing field of oncolytic virus (OV) therapy necessitates the development of new and improved analytical approaches for the characterization of the virus during production and development.
Rajeswari Basu, Richard Dambra, Di Jiang, Sophia A. Schätzlein, Shu Njiyang, Joseph Ashour, Abhilash I. Chiramel, Adam Vigil, Vladimir V. Papov   +8 more
doaj   +1 more source

Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection. [PDF]

Sci Rep, 2022
Bezerra CS, Portilho MM, Barbosa JR, de Azevedo CP, Mendonça ACDF, da Cruz JNM, Frota CC, do Lago BV, Villar LM.   +8 more
europepmc   +1 more source