Results 331 to 340 of about 3,690,281 (384)

Infectious Viral Quantification of Chikungunya Virus-Virus Plaque Assay.

Methods in molecular biology, 2016
The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via
Parveen Kaur, Regina Ching Hua Lee, J. Chu   +2 more
semanticscholar   +3 more sources

Virus Propagation and Cell-Based Colorimetric Quantification

Journal of Visualized Experiments, 2023
Zika virus (ZIKV) is a mosquito-borne virus belonging to the genus Flavivirus. ZIKV infection has been associated with congenital brain abnormalities and potentially Guillain-Barré syndrome in adults. Research on ZIKV to understand the disease mechanisms is important to facilitate vaccine and treatment development.
Tan, Jia-Yi, Wong, Jo -Ern, Zainal, Nurhafiza, AbuBakar, Sazaly, Tan, Kim-Kee   +4 more
openaire   +3 more sources

Rapid Airborne Influenza Virus Quantification Using an Antibody-Based Electrochemical Paper Sensor and Electrostatic Particle Concentrator.

Environmental Science and Technology, 2020
Airborne influenza viruses are responsible for serious respiratory diseases, and most detection methods for airborne viruses are based on extraction of nucleic acids.
Jyoti Bhardwaj, M. Kim, Jaesung Jang
semanticscholar   +1 more source

Review of Detection and Quantification of Rabies Virus Antibodies

Viral Immunology, 2021
Rabies is an almost invariably fatal disease. According to the World Health Organization (WHO), rabies virus neutralizing antibody (RVNA) titers of ≥0.5 IU/mL are considered adequate for rabies protection. Therefore, detection and quantification of RABV antibodies are important. Many methods have been developed for detecting RABV antibodies.
Kewen Su, Jian Xue, Xiaoyue Shan, Haipeng Ye, Ling Zhang, Siwei Tan, Ji Shao, Yanpeng Shi, Zhe Wang, Lei Zhang   +9 more
openaire   +2 more sources

Sindbis Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2005
AbstractThe prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, Sindbis virus shuttles from an insect vector to a vertebrate host and back to the insect vector in a complex transmission cycle. Sindbis virus must, therefore, be able to replicate in two biochemically and genetically divergent hosts, invertebrates and ...
Christine Sinodis, Raquel Hernandez, Dennis Brown   +2 more
openaire   +3 more sources

Newcastle Disease Virus: Propagation, Quantification, and Storage [PDF]

Current Protocols in Microbiology, 2006
AbstractNewcastle disease virus (NDV) is a prototype paramyxovirus used to define basic steps in the life cycle of this family of viruses. NDV is also an ideal virus system for elucidating determinants of viral pathogenicity. Some strains of this virus are important agricultural pathogens that cause disease in poultry with a high mortality while other ...
Julie N. Reitter, Trudy G. Morrison, Homer Pantua, Lori W. McGinnes   +3 more
openaire   +2 more sources

Dengue Virus: Isolation, Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2012
AbstractDengue is a disease caused by infection with one of the four dengue virus serotypes (DENV‐1, ‐2, ‐3, and ‐4). The virus is transmitted to humans by Aedes sp. mosquitoes. This enveloped virus contains a positive single‐stranded RNA genome. Clinical manifestations of dengue can have a wide range of outcomes varying from a mild febrile illness to ...
Freddy A. Medina, Jorge L. Muñoz-Jordán, Edgardo Vergne, Juan F. Medina, Gilberto A. Santiago, Candimar Colón   +5 more
openaire   +3 more sources

Herpes Simplex Virus: Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2006
AbstractHerpes simplex virus (HSV) is the prototype of a family of large, enveloped, double‐stranded DNA viruses, the Herpesviridae, which cause significant morbidity and mortality in humans. Productive replication of HSV in cells in culture results in definitive changes in cellular physiology and metabolism, ultimately leading to lysis.
John A. Blaho, Elise R. Morton, Jamie C. Yedowitz   +2 more
openaire   +2 more sources

Hemorrhagic Enteritis in Turkeys: Purification and Quantification of the Virus

Avian Diseases, 1983
Two methods for purifying the virus of hemorrhagic enteritis from infected turkey spleens are described. One procedure utilized precipitation with polyethylene glycol, and the other consisted of trichlorotrifluoroethane extraction. Both procedures included sucrose-cesium chloride gradient centrifugation in the final purification step.
G G Schurig, R C Bates, J E Ossa
openaire   +3 more sources