Results 341 to 350 of about 3,690,281 (384)

Analytical strategies for quantification of adeno-associated virus (AAV) empty capsids to support process development.

Human Gene Therapy Methods, 2019
Recombinant adeno-associated virus (rAAV)-mediated gene therapy is a fast-evolving field in the biotechnology industry. One of the major challenges in developing a purification process for AAV gene therapy is establishing an effective yet scalable method
Xiaotong Fu, Wei-Chiang Chen, Chris Argento, Ryan Dickerson, Pete Clarner, V. Bhatt, G. Bou-Assaf, Meisam Bakhshayeshi, Xiaohui Lu, S. Bergelson, John Pieracci   +10 more
semanticscholar   +1 more source

Experimental quantification of transmission of genetically engineered pseudorabies virus

Vaccine, 1995
There is concern that live pseudorabies virus (PRV) vaccine or PRV vector vaccine strains may spread from vaccinated to unvaccinated pigs. Moreover, it is feared that recombining PRV vaccine strains with related vaccine or wild-type strains may lead to spread and survival of recombinant PRV.
Mulder, W.A.M., de Jong, M.C.M., Priem, J., Bouma, A., Pol, J.M.A., Kimman, T.G.   +5 more
openaire   +3 more sources

Experimental quantification of vaccine-induced reduction in virus transmission

Vaccine, 1994
Although reduction in transmission of an agent in the host population is an important goal of many vaccinations, suitable experimental methods to measure transmission have been lacking. Therefore, we designed and tested an animal experiment to quantify transmission among vaccinated and unvaccinated animals.
Mart C.M. de Jong, Tjeerd G. Kimman
openaire   +3 more sources

Propagation, Quantification, Detection, and Storage of West Nile Virus

Current Protocols in Microbiology, 2013
AbstractWest Nile virus (WNV) is a member of the Flaviviridae family of enveloped, single‐stranded, positive‐sense RNA viruses. WNV, an emerging viral pathogen, is transmitted by mosquitoes to birds and mammals and is responsible for an increasing incidence of human disease in North America and Europe.
James D. Brien, Helen M. Lazear, Michael S. Diamond   +2 more
openaire   +3 more sources

Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification

Current Protocols in Microbiology, 2018
AbstractPorcine reproductive and respiratory syndrome virus (PRRSV) is a member of the familyArteriviridae, orderNidovirale. PRRSV is an enveloped, single‐stranded, positive‐sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC‐145 cell line is the most often used in a laboratory setting.
Liping Yang, Yan-Jin Zhang, Zexu Ma
openaire   +3 more sources

Quantification of West Nile Virus by Plaque-Forming Assay

2022
The plaque-forming assay is a gold standard technique to determine the concentration of infectious viral particles. In this assay, lytic viruses infect and lyse the cells but are immobilized due to the presence of an agarose-containing overlay medium.
Neupane, Biswas, Bai, Fengwei
openaire   +3 more sources

Quantification of Cell Turnover in the Bovine Leukemia Virus Model

2017
In a perspective of a comparative virology approach, characterization of the bovine leukemia virus (BLV) model may be helpful to better understand infection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle.
de Brogniez, Alix, Barez, Pierre-Yves, Carpentier, Alexandre, Gutiérrez, Gerónimo, Reichert, Michal, Trono, Karina Gabriela, Willems, Luc   +6 more
openaire   +4 more sources

Bluetongue Virus (BTV): Propagation, Quantification, and Storage

Current Protocols in Microbiology, 2012
AbstractAs an obligate intracellular parasite, the genome of the Bluetongue virus (BTV) contains ten double‐stranded RNA segments which are encapsidated by viral proteins, forming “transport vesicles” that can transmit the viral progeny cell‐to‐cell efficiently and that can also be transmitted animal‐to‐animal by a biting midge.
openaire   +3 more sources

Serial quantification of the human immunodeficiency virus in an arthroscopic effluent

Arthroscopy: The Journal of Arthroscopic & Related Surgery, 1997
We describe a case in which a patient infected with the human immunodeficiency virus (HIV) with high plasma viral titers underwent an arthroscopic partial meniscectomy allowing us the opportunity to measure the HIV titer of serial aliquots of the arthroscopic effluent using the polymerase chain reaction.
Matthew J. Matava, Mary M. Horgan
openaire   +3 more sources

Quantification of the Removal and Inactivation of Virus Particles

Researchers from the Netherlands have developed and demonstrated a new method of quantifying and characterizing antiviral properties of polymer-functionalized surfaces for virus filtration and inactivation. Specifically, a polyethylenimine (PEI)-coated poly(ether sulfone) (PES) micro-filtration membrane was used to adsorb, inactivate, as well as ...
Jeremiah Hashley, Lisa Mueller
openaire   +1 more source