Results 341 to 350 of about 3,645,587 (361)
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Experimental quantification of vaccine-induced reduction in virus transmission
Vaccine, 1994Although reduction in transmission of an agent in the host population is an important goal of many vaccinations, suitable experimental methods to measure transmission have been lacking. Therefore, we designed and tested an animal experiment to quantify transmission among vaccinated and unvaccinated animals.
Mart C.M. de Jong, Tjeerd G. Kimman
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Propagation, Quantification, Detection, and Storage of West Nile Virus
Current Protocols in Microbiology, 2013AbstractWest Nile virus (WNV) is a member of the Flaviviridae family of enveloped, single‐stranded, positive‐sense RNA viruses. WNV, an emerging viral pathogen, is transmitted by mosquitoes to birds and mammals and is responsible for an increasing incidence of human disease in North America and Europe.
James D. Brien +2 more
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Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification
Current Protocols in Microbiology, 2018AbstractPorcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family Arteriviridae, order Nidovirale. PRRSV is an enveloped, single‐stranded, positive‐sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC‐145 cell line is the most often used in a laboratory setting.
Liping Yang, Yan-Jin Zhang, Zexu Ma
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Quantification of West Nile Virus by Plaque-Forming Assay
2022The plaque-forming assay is a gold standard technique to determine the concentration of infectious viral particles. In this assay, lytic viruses infect and lyse the cells but are immobilized due to the presence of an agarose-containing overlay medium.
Neupane, Biswas, Bai, Fengwei
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Bluetongue Virus (BTV): Propagation, Quantification, and Storage
Current Protocols in Microbiology, 2012AbstractAs an obligate intracellular parasite, the genome of the Bluetongue virus (BTV) contains ten double‐stranded RNA segments which are encapsidated by viral proteins, forming “transport vesicles” that can transmit the viral progeny cell‐to‐cell efficiently and that can also be transmitted animal‐to‐animal by a biting midge.
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Quantification of Cell Turnover in the Bovine Leukemia Virus Model
2017In a perspective of a comparative virology approach, characterization of the bovine leukemia virus (BLV) model may be helpful to better understand infection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle.
de Brogniez, Alix +6 more
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Serial quantification of the human immunodeficiency virus in an arthroscopic effluent
Arthroscopy: The Journal of Arthroscopic & Related Surgery, 1997We describe a case in which a patient infected with the human immunodeficiency virus (HIV) with high plasma viral titers underwent an arthroscopic partial meniscectomy allowing us the opportunity to measure the HIV titer of serial aliquots of the arthroscopic effluent using the polymerase chain reaction.
Matthew J. Matava, Mary M. Horgan
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Phytopathology, 2003
Reliable detection and quantification of barley and cereal yellow dwarf viruses (YDVs) is a critical component in managing yellow dwarf diseases in small grain cereal crops.
B. Balaji +2 more
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Reliable detection and quantification of barley and cereal yellow dwarf viruses (YDVs) is a critical component in managing yellow dwarf diseases in small grain cereal crops.
B. Balaji +2 more
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Quantification of the Removal and Inactivation of Virus Particles
Researchers from the Netherlands have developed and demonstrated a new method of quantifying and characterizing antiviral properties of polymer-functionalized surfaces for virus filtration and inactivation. Specifically, a polyethylenimine (PEI)-coated poly(ether sulfone) (PES) micro-filtration membrane was used to adsorb, inactivate, as well as ...Jeremiah Hashley, Lisa Mueller
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Uncertainty quantification in the Brazilian outbreak of Zika virus
2018Several instances of Zika virus epidemic have been reported around the world in the last 20 years, causing Zika fever to become a disease of international concern. The use of mathematical models for epidemics in this context very important, once they are useful tools to predict the outbreaks underlying numbers, and allow one to test the effectiveness ...
Cunha Jr, Americo +2 more
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