Results 121 to 130 of about 695,432 (264)
FEBS Open Bio, EarlyView.In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes +32 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
, 2017 Dimitrios Karagiannis,1 Irini Chatziralli,1 Konstantinos Kaprinis,1 Ilias Georgalas,2 Efstratios Parikakis,1 Panagiotis Mitropoulos1 1Second Department of Ophthalmology, Ophthalmiatrion Athinon, Athens, Greece; 2First Department of Ophthalmology ...
Chatziralli I +5 more
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Screening older people for impaired vision [PDF]
A systematic review of trials about screening older people for visual impairment found no evidence that screening improved vision. We undertook a new trial nested within a larger cluster randomised trial of multidimensional ...
Smeeth, Liam
core +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Self-reported independent living outcome measures of blind elders attending adjustment training [PDF]
, 2002 Plan BThis descriptive study was a 2-year follow up of Adjustment to Blindness training and filled gaps in the literature by using self-report to measure an array of adjustment to blindness outcomes.
Van Tol, Ruth R.
core
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Visual outcome of cataract surgery
, 2013 The aim of this study was to assess whether the visual outcome of cataract surgery in the institution was in accordance with the World Health Organization recommendations.
Khan, Qazi Assad +3 more
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Purpose: To evaluate long-term refractive, visual outcomes and patient satisfaction following capsular bag implantation of a multifocal toric intraocular lens (IOL). Setting: Department of Ophthalmology, Professor K. Gibinski University Clinical Center,
Agnieszka Malec-Pleskacz +2 more
core +1 more source