Results 191 to 200 of about 12,666 (230)
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Determination of locust vitellogenin by radioimmunoassay with [3H]propionyl-vitellogenin
General and Comparative Endocrinology, 1983A simple procedure for radiolabeling of locust vitellogenin is described. This procedure involves coupling of [3H]propionyl succinimidate to purified vitellogenin with high yield and specific activity. Using this radiolabeled analog, a specific and sensitive radioimmunoassay was developed for determining locust vitellogenin content, with a lower ...
C, Asher +2 more
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Vitellogenin – a biomarker for endocrine disruptors
TrAC - Trends in Analytical Chemistry, 1998The increasing preponderance of females in fish populations has become a matter of concern not only for scientists but also for environmental authorities. For example, in the extensive waterways in Berlin, Germany, 70% of the fish population is female.
P.-D Hansen +6 more
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Functions of Vitellogenin in Eggs
2017Our understanding of the functions of vitellogenin (Vtg) in reproduction has undergone an evolutionary transformation over the past decade. Primarily, Vtg was regarded as a female-specific reproductive protein, which is cleaved into yolk proteins including phosvitin (Pv) and lipovitellin (Lv), stored in eggs, providing the nutrients for early embryos ...
Hongyan, Li, Shicui, Zhang
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
Abstract Polysomes from estrogenized rooster liver were subfractionated on sucrose gradients. The size distribution of the vitellogenin polysomes was determined with in vitro labelling of the nascent chains followed by immunochemical determination of the elongated vitellogenin chains. Vitellogenin polysomes contain 30–40 ribosomes.
W G, Roskam, M, Gruber, G, Ab
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Abstract Polysomes from estrogenized rooster liver were subfractionated on sucrose gradients. The size distribution of the vitellogenin polysomes was determined with in vitro labelling of the nascent chains followed by immunochemical determination of the elongated vitellogenin chains. Vitellogenin polysomes contain 30–40 ribosomes.
W G, Roskam, M, Gruber, G, Ab
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Evolutionary Conservation of Vitellogenin Genes
DNA, 1982Homologous and heterologous hybridizations in solution were performed between sheared genomic DNA and DNA complementary to vitellogenin mRNA of Xenopus, chicken, and migratory locust. The kinetics of hybridization and the thermal stability of the hybrids formed suggested a high degree of conservation of coding sequences of insect, amphibian, and avian ...
T C, James +4 more
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Vitellogenesis and the Vitellogenin Gene Family
Science, 1981Vitellogenin is synthesized under estrogen control in the liver, extensively modified, transported to the ovary, and there processed to the yolk proteins lipovitellin and phosvitin. In the frog Xenopus laevis there are at least four distinct but related vitellogenin genes.
W, Wahli +3 more
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Molecular characteristics of insect vitellogenins
Journal of Insect Physiology, 2008Vitellogenins (Vgs) are precursors of the major egg storage protein, vitellin (Vn), in many oviparous animals. Insects Vgs are large molecules ( approximately 200-kD) synthesized in the fat body in a process that involves substantial structural modifications (e.g., glycosylation, lipidation, phosphorylation, and proteolytic cleavage, etc.) of the ...
Muhammad, Tufail, Makio, Takeda
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Molecular and Cellular Endocrinology, 1978
Explants of male Xenopus liver maintained in a serum-free culture medium respond to stimulation by 2 X 10(-8) M 17beta-estradiol with an increasing rate of accumulation of vitellogenin mRNA, as revealed by hybridization of cDNA to the total cytoplasmic RNA extracted from the cultures.
B K, Felber, G U, Ryffel, R, Weber
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Explants of male Xenopus liver maintained in a serum-free culture medium respond to stimulation by 2 X 10(-8) M 17beta-estradiol with an increasing rate of accumulation of vitellogenin mRNA, as revealed by hybridization of cDNA to the total cytoplasmic RNA extracted from the cultures.
B K, Felber, G U, Ryffel, R, Weber
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Insect Biochemistry and Molecular Biology, 1994
Athalia rosae (Hymenoptera) was previously shown to have two vitellins (L-Vn and S-Vn) and the two corresponding vitellogenins (L-Vg and S-Vg). A cDNA expression library was constructed from poly(A)+ RNA prepared from adult female fat body cells, and was screened for the vitellogenins by using antisera against the L- and S-Vn, respectively ...
Y, Kageyama +4 more
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Athalia rosae (Hymenoptera) was previously shown to have two vitellins (L-Vn and S-Vn) and the two corresponding vitellogenins (L-Vg and S-Vg). A cDNA expression library was constructed from poly(A)+ RNA prepared from adult female fat body cells, and was screened for the vitellogenins by using antisera against the L- and S-Vn, respectively ...
Y, Kageyama +4 more
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Gene, 2000
By analysis of zebrafish EST (expressed sequence tag) clones from an adult cDNA library, we have identified 44 clones, about 11% of the adult EST clones, encoding vitellogenins. These vitellogenin EST clones have been derived from at least seven distinct vitellogenin genes.
Wang, H., Yan, T., Tan, J.T.T., Gong, Z.
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By analysis of zebrafish EST (expressed sequence tag) clones from an adult cDNA library, we have identified 44 clones, about 11% of the adult EST clones, encoding vitellogenins. These vitellogenin EST clones have been derived from at least seven distinct vitellogenin genes.
Wang, H., Yan, T., Tan, J.T.T., Gong, Z.
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