Results 71 to 80 of about 935,045 (283)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Calculation Method of Phase Interfacial Parameter for Churn Flow and Annular Flow in Narrow Rectangular Channel

Yuanzineng kexue jishu, 2022
The void fraction and interfacial area concentration are important phase interfacial parameters in two-phase flow. Accurate acquisition of void fraction and interfacial area concentration under churn flow and annular flow in a narrow rectangular channel
CHENG Linhai;GU Haifeng;LIU Antai;YU Xiang;ZHANG Junyi
doaj  

An improved electrical-conductance sensor for void-fraction measurement in a horizontal pipe

Nuclear Engineering and Technology, 2015
The electrical-impedance method has been widely used for void-fraction measurement in two-phase flow due to its many favorable features. In the impedance method, the response characteristics of the electrical signal heavily depend upon flow pattern, as ...
Min Seok Ko, Bo An Lee, Woo Youn Won, Yeon Gun Lee, Dong Wook Jerng, Sin Kim   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Experimental Research and Numerical Simulation on Gas-Liquid Separation Performance at High Gas Void Fraction of Helically Coiled Tube Separator

International Journal of Chemical Engineering, 2014
The industrial removal process of the light hydrocarbon and water from wet natural gas can be simulated in laboratory with the independently designed helically coiled tube gas-liquid separator.
Yongxue Zhang, Chan Guo, Hucan Hou, Guomin Xue   +3 more
doaj   +1 more source

Three dimensional analysis of temperature effect on control rod worth in TRR

Nuclear Engineering and Technology, 2018
In this paper, three-dimensional neutronic calculations were performed in order to calculate the dependency of CRW on the temperature of fuel and moderator and the moderator void.
Maedeh Yari, Ahmad Lashkari, S. Farhad Masoudi, Mirshahram Hosseinipanah   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Experimental investigation of the spatial evolution of local flow parameters in vertical LBE–N2 bubbly flow

International Journal of Advanced Nuclear Reactor Design and Technology
To support closure-model development for thermal-hydraulic analysis of SGTR/HXTR-related two-phase flow in lead-cooled fast reactors, a separate-effect experimental study was conducted on lead–bismuth eutectic (LBE)–nitrogen bubbly flow in a vertical ...
Zihao Zhu, Bin Li, Bin Hou, Lina Zhu, Kefan Geng, Bo Pang, Yangbin Deng, Xi Huang   +7 more
doaj   +1 more source

Numerical Study of the Steady-State Subchannel Test-Case with NEPTUNE_CFD for the OECD/NRC NUPEC PSBT Benchmark

Science and Technology of Nuclear Installations, 2012
The multifield computational fluid dynamics (CFD) code NEPTUNE_CFD is applied to carry out a numerical study of the steady-state subchannel test-case of the OECD/NRC NUPEC PWR subchannel and bundle tests (PSBTs) international benchmark, focusing on the ...
C. Baudry, M. Guingo, A. Douce, J. Laviéville, S. Mimouni, M. Boucker   +5 more
doaj   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source