We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
CeO2:Mn3O4 Catalytic Micro-Converters Tuned for CH4 Detection Based on Catalytic Combustion under Real Operating Conditions. [PDF]
Simion CE +6 more
europepmc +1 more source
Integrated PbS Colloidal Quantum Dot Photodiodes on Silicon Nitride Waveguides. [PDF]
Pang C +9 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Characterization of the Advanced Stirling Radioisotope Generator Engineering Unit 2 [PDF]
Significant progress was made developing the Advanced Stirling Radioisotope Generator (ASRG) 140-W radioisotope power system. While the ASRG flight development project has ended, the hardware that was designed and built under the project is continuing to
Oriti, Salvatore M. +2 more
core +1 more source
Switched-capacitor-convertors based on fractal design for output power management of triboelectric nanogenerator. [PDF]
Liu W +10 more
europepmc +1 more source
Magnetostrictive and Magnetoactive Effects in Piezoelectric Polymer Composites. [PDF]
Stolbov OV, Raikher YL.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Characterization of the Advanced Stirling Radioisotope Generator EU2 [PDF]
Significant progress was made developing the Advanced Stirling Radioisotope Generator (ASRG), a 140-watt radioisotope power system. While the ASRG flight development project has ended, the hardware that was designed and built under the project is ...
Oriti, Salvatore M. +2 more
core +1 more source
Real-time numerical system convertor via two-dimensional WS2-based memristive device. [PDF]
Xin X +10 more
europepmc +1 more source

