SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
An electric spring with an extended range of operation and current filtering capability for SEIG-based isolated system. [PDF]
Pati S, Nanda AB, Choudhury A, Sahu BK.
europepmc +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
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Research on a Phase-Shift-Based Discontinuous PWM Method for 24V Onboard Thermally Limited Micro Voltage Source Inverters. [PDF]
Wang S, Xia C.
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What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman +4 more
wiley +1 more source
An Adaptive Control Strategy for DC/DC Converters Using Command-Filtered Backstepping and Disturbance Rejection. [PDF]
Phan VD, Duong DT, Le VC, Ho SP.
europepmc +1 more source
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso +4 more
wiley +1 more source
Design of Dual Continuous-Mode Class-J Power Amplifiers with Harmonic Matching Networks for X and Ku Bands. [PDF]
Yuan Y, Zhao X, Fan J, Yu Z.
europepmc +1 more source
Interleaved Quadratic Boost DC-DC Converter with Extended Voltage Gain and Reduced Switch Voltage Stress for Photovoltaic Applications. [PDF]
Ferreira D +7 more
europepmc +1 more source
Adaptive non unit traveling wave protection algorithm for multi terminal HVDC grids. [PDF]
Raza A +6 more
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