Educação, trabalho e reforma: Azimutes do envelhecimento [PDF]
A longevidade é entendida como um direito e uma oportunidade e as experiências educativas desempenham um papel fundamental no garante da dignidade e da participação social dos indivíduos.
Rebelo, B. +3 more
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
[Recommendations and Protocols for the Safe Use of Tourniquets in Upper- and Lower-Extremity Surgeries]. [PDF]
Neder Filho AT, Campos TVO.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
[CSP in 40 years of scientific publications]. [PDF]
Lima LD, Carvalho MS, Alves LC.
europepmc +1 more source
Economic evaluation of an active search system to monitor the outcomes of health-related claims. [PDF]
Rodrigues NLP +4 more
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Internal and marginal discrepancies of monolithic zirconia, polymer infiltrated ceramic, and lithium disilicate crowns: an in vivo study. [PDF]
Miranda LM +6 more
europepmc +1 more source
Brazilian Society of Cardiology Guideline on Myocarditis - 2022. [PDF]
Montera MW +44 more
europepmc +1 more source

