Ubiquitin Ligase SMURF2 Interacts with Filovirus VP40 and Promotes Egress of VP40 VLPs [PDF]
Viruses, 2021 Filoviruses Ebola (EBOV) and Marburg (MARV) are devastating high-priority pathogens capable of causing explosive outbreaks with high human mortality rates.
Ariel Shepley-McTaggart +7 more
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Domain rearrangement and denaturation in Ebola virus protein VP40 [PDF]
AIP Advances, 2018 The VP40 protein plays a critical role in coordinating the virion assembly, budding, and replication of the Ebola virus. Efforts have been made in recent years to understand various aspects of VP40 structure, dynamics, and function such as assembly of ...
Rudramani Pokhrel +5 more
doaj +3 more sources
Obtaining and Immunologic Characterization of Filoviruses Recombinant Proteins VP40 and NP
Проблемы особо опасных инфекций, 2010 Full-size genes coding matrix proteins VP40 of Ebola virus (VE) and Marburg virus (VM) and nucleoprotein (NP) of VE were cloned. These genes expression in Escherichia coli and purification of their products by affinity chromatography using Ni-NTA agarose
A. V. Ivanova +7 more
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A Loop Region in the N-Terminal Domain of Ebola Virus VP40 Is Important in Viral Assembly, Budding, and Egress [PDF]
Viruses, 2014 Ebola virus (EBOV) causes viral hemorrhagic fever in humans and can have clinical fatality rates of ~60%. The EBOV genome consists of negative sense RNA that encodes seven proteins including viral protein 40 (VP40).
Emmanuel Adu-Gyamfi +5 more
doaj +4 more sources
Cysteine Mutations in the Ebolavirus Matrix Protein VP40 Promote Phosphatidylserine Binding by Increasing the Flexibility of a Lipid-Binding Loop [PDF]
Viruses, 2021 Ebolavirus (EBOV) is a negative-sense RNA virus that causes severe hemorrhagic fever in humans. The matrix protein VP40 facilitates viral budding by binding to lipids in the host cell plasma membrane and driving the formation of filamentous, pleomorphic ...
Kristen A. Johnson +7 more
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Membrane Binding and Bending in Ebola VP40 Assembly and Egress [PDF]
Frontiers in Microbiology, 2014 Lipid-enveloped viruses contain a lipid bilayer coat that protects their genome and helps to facilitate entry into the host cell. Filoviruses are lipid-enveloped viruses that have up to 90% clinical fatality and include Marbug (MARV) and Ebola (EBOV ...
Robert V Stahelin, Robert V Stahelin
doaj +3 more sources
Ebola VP40 in Exosomes Can Cause Immune Cell Dysfunction [PDF]
Frontiers in Microbiology, 2016 Ebola virus (EBOV) is an enveloped, ssRNA virus from the family Filoviridae capable of causing severe hemorrhagic fever with up to 80–90% mortality rates.
Michelle L. Pleet +16 more
doaj +3 more sources
The Role of Exosomal VP40 in Ebola Virus Disease [PDF]
DNA and Cell Biology, 2017 Ebola virus (EBOV) can cause a devastating hemorrhagic disease, leading to death in a short period of time. After infection, the resulting EBOV disease results in high levels of circulating cytokines, endothelial dysfunction, coagulopathy, and bystander lymphocyte apoptosis in humans and nonhuman primates.
Michelle L. Pleet +4 more
openalex +3 more sources
Ebola Virus VP40-Induced Particle Formation and Association with the Lipid Bilayer [PDF]
Journal of Virology, 2001 ABSTRACT Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle. To elucidate the functions of VP40, we investigated its ability to induce the formation of membrane-bound particles when it was expressed apart from other viral proteins.
Luke D. Jasenosky +3 more
openalex +3 more sources
Alphavirus Replicon DNA Vectors Expressing Ebola GP and VP40 Antigens Induce Humoral and Cellular Immune Responses in Mice [PDF]
Frontiers in Microbiology, 2018 Ebola virus (EBOV) causes severe hemorrhagic fevers in humans, and no approved therapeutics or vaccine is currently available. Glycoprotein (GP) is the major protective antigen of EBOV, and can generate virus-like particles (VLPs) by co-expression with ...
Shoufeng Ren +14 more
doaj +2 more sources