Results 131 to 140 of about 6,175 (141)

A ph controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA 1 1Edited by I. Tinoco

Journal of Molecular Biology, 2001
The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+.
Thorsten Dieckmann, Jeremy Flinders
openaire   +3 more sources

Identification of the Catalytic Subdomain of the VS Ribozyme and Evidence for Remarkable Sequence Tolerance in the Active Site Loop

Journal of Molecular Biology, 2002
We show here that the ribozyme domain of the Neurospora VS ribozyme consists of separable upper and lower subdomains. Deletion analysis demonstrates that the entire upper subdomain (helices III/IV/V) is dispensable for site-specific cleavage activity, providing experimental evidence that the active site is contained within the lower subdomain and ...
Vanita D. Sood, Richard A. Collins
openaire   +3 more sources

Spermine Switches a Neurospora VS Ribozyme from Slow Cis Cleavage to Fast Trans Cleavage

Biochemistry, 1998
In keeping with the known role of polyamines as counterions in RNA folding, we have found that concentrations of spermine as low as 1 microM facilitate first-order Cis cleavage and decrease the concentration of magnesium required for optimal cleavage of the VS ribozyme.
Richard A. Collins, Joan E. Olive
openaire   +2 more sources

Identification of phosphate groups involved in metal binding and tertiary interactions in the core of the Neurospora VS ribozyme 1 1Edited by D. Draper

Journal of Molecular Biology, 1998
We have used ethylation protection experiments and modification interference using phosphorothioate nucleosides to identify phosphate groups involved in the magnesium-dependent tertiary structure and function of the VS ribozyme, a small, self-cleaving RNA.
Tara L. Beattie, Vanita D. Sood, Richard A. Collins   +2 more
openaire   +3 more sources

Functional equivalence of the uridine turn and the hairpin as building blocks of tertiary structure in the Neurospora VS ribozyme 1 1Edited by D. Draper

Journal of Molecular Biology, 2001
Mutational, kinetic, and chemical modification experiments show that one of the three-way helical junctions in the Neurospora VS ribozyme contains a uridine turn that is important for organizing the functional three-dimensional structure of this junction.
Vanita D. Sood, Richard A. Collins
openaire   +3 more sources

Identification of Separate Structural Features That Affect Rate and Cation Concentration Dependence of Self-Cleavage by the Neurospora VS Ribozyme

Biochemistry, 2006
The cleavage site of the Neurospora VS ribozyme is located in an internal loop in a hairpin called stem-loop I. Stem-loop I undergoes a cation-dependent structural change to adopt a conformation, termed shifted, that is required for activity. Using site-directed mutagenesis and kinetic analyses, we show here that the insertion of a single-stranded ...
Alan H. L. Poon, Joan E. Olive, Meredith Mclaren, Richard A. Collins   +3 more
openaire   +3 more sources

Kinetic studies of the Neurospora VS ribozyme

Collection Symposium Series, 2002
Iain A. Murray, Svetomir B. Tzokov, Yana S. Kovacheva, Jane A. Grasby   +3 more
openaire   +2 more sources

Études structurales et ingénierie du ribozyme VS de Neurospora

2021
Les ARN non-codants exercent des rôles essentiels au sein de nombreux processus biologiques, allant de la régulation de l’expression génique à l’activité enzymatique. Afin de remplir leurs fonctions cellulaires, ces ARN doivent adopter des structures tridimensionnelles spécifiques, et mieux comprendre ces structures et leur dynamique est crucial pour ...
openaire   +1 more source

Faculty Opinions recommendation of The global structure of the VS ribozyme.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature, 2002
openaire   +1 more source

Faculty Opinions recommendation of A guanine nucleobase important for catalysis by the VS ribozyme.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature, 2007
openaire   +1 more source