RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins. [PDF]
, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
LIU, Fenyong +9 more
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Lipid vesicles chaperone an encapsulated RNA aptamer. [PDF]
, 2018 The organization of molecules into cells is believed to have been critical for the emergence of living systems. Early protocells likely consisted of RNA functioning inside vesicles made of simple lipids.
Chen, Irene A +2 more
core +1 more source
A Faster Triphosphorylation Ribozyme. [PDF]
, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Akoopie, Arvin +2 more
core +4 more sources
Selection of chromosomal DNA libraries using a multiplex CRISPR system. [PDF]
, 2014 The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that ...
Arkin, Adam P +10 more
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The prebiotic evolutionary advantage of transferring genetic information from RNA to DNA. [PDF]
, 2011 In the early 'RNA world' stage of life, RNA stored genetic information and catalyzed chemical reactions. However, the RNA world eventually gave rise to the DNA-RNA-protein world, and this transition included the 'genetic takeover' of information storage ...
Chen, Irene A +4 more
core +4 more sources
Intramolecular secondary structure rearrangement by the kissing interaction of the Neurospora VS ribozyme [PDF]
Proceedings of the National Academy of Sciences, 2001 Kissing interactions in RNA are formed when bases between two hairpin loops pair. Intra- and intermolecular kissing interactions are important in forming the tertiary or quaternary structure of many RNAs. Self-cleavage of the wild-type Varkud satellite (VS) ribozyme requires a kissing interaction between the hairpin loops of stem-loops I and V.
Angela A. Andersen, Richard A. Collins
openaire +3 more sources
Focus on Function: Single Molecule Enzymology of the VS ribozyme
The FASEB Journal, 2008 Non‐coding RNAs of complex structure are involved in numerous aspects of the replication and processing of genetic information. Within the mitochondria of the bread mold Neurospora, the Varkud satellite (VS) RNA replicates parasitically by cycling between DNA and multimeric RNA forms.
Miguel J. B. Pereira +2 more
openaire +2 more sources
Encoding folding paths of RNA switches [PDF]
, 2006 RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions.
Harlepp, S. +3 more
core +3 more sources
4-thio-U cross-linking identifies the active site of the VS ribozyme [PDF]
The EMBO Journal, 2002 To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide analog, 4-thio- uridine, immediately downstream of the scissile bond. Exposure to UV light produced two cross-linked RNAs, in which the 4-thio-uridine was cross-
Richard A. Collins +3 more
openaire +3 more sources
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]
, 2020 Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL +3 more
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