Results 61 to 70 of about 6,175 (141)

Targeted insertion of large DNA fragments through template‐jumping prime editing in rice

Plant Biotechnology Journal, EarlyView.
Fei Li, Haonan Hou, Minglei Song, Zhen Chen, Ting Peng, Yanxiu Du, Yafan Zhao, Junzhou Li, Chunbo Miao   +8 more
wiley   +1 more source

Evolution Driven Microscale Combinatorial Chemistry in Intracellular Mimicking Droplets to Engineer Thermostable RNA for Cellular Imaging

Small, Volume 21, Issue 9, March 5, 2025.
Microfluidic‐based directed evolution is a powerful tool for selection of biopolymers, however they struggle in transferability to intracellular environments. The “biomimetic equivalence” principle provides a versatile and practical framework for developing biopolymers that function effectively in cells using a cell‐free approach.
Andrew Brian Kinghorn, Wei Guo, Lin Wang, Matthew Yuk Heng Tang, Fang Wang, Simon Chi‐Chin Shiu, Kwan Kiu Lau, Chandra Jinata, Aditi Dey Poonam, Ho Cheung Shum, Julian Alexander Tanner   +10 more
wiley   +1 more source

An important role of G638 in the cis-cleavage reaction of the Neurospora VS ribozyme revealed by a novel nucleotide analog incorporation method [PDF]

RNA, 2008
We describe a chemical coupling procedure that allows joining of two RNAs, one of which contains a site-specific base analog substitution, in the absence of divalent ions. This method allows incorporation of nucleotide analogs at specific positions even into large, cis-cleaving ribozymes. Using this method we have studied the effects of substitution of
Reza Mehdizadeh, Dominic C. J. Jaikaran, Richard A. Collins, Joan E. Olive, M. Duane Smith   +4 more
openaire   +3 more sources

Études d'ingénierie du ribozyme VS de Neurospora [PDF]

, 2015
Les ribozymes sont des ARN catalytiques fréquemment exploités pour le développement d’outils biochimiques et d’agents thérapeutiques. Ils sont particulièrement intéressants pour effectuer l’inactivation de gènes, en permettant la dégradation d’ARNm ou
Lacroix-Labonté, Julie
core  

In vitro transactivation of Bacillus subtilis RNase P RNA [PDF]

, 2001
Deletion of the ‘signature’ PL5.1 stem-loop structure of a Type II RNase P RNA diminished its catalytic activity. Addition of PL5.1 in trans increased catalytic efficiency (kcat/KM) rather than kcat. Transactivation was due to the binding of a single PL5.
Kim, Hadong, Leeper, Thomas C, Meyer, Melissa A, Poelling, Richard R, Schmidt, Francis J   +4 more
core   +1 more source

Ribozymes: the characteristics and properties of catalytic RNAs [PDF]

, 2017
Ribozymes, or catalytic RNAs, were discovered a little more than 15 years ago. They are found in the organelles of plants and lower eukaryotes, in amphibians, in prokaryotes, in bacteriophages, and in viroids and satellite viruses that infect plants.
Tanner, N. Kyle
core  

Intramolecular Phenotypic Capacitance in a Modular RNA Molecule [PDF]

, 2015
Phenotypic capacitance refers to the ability of a genome to accumulate mutations that are conditionally hidden and only reveal phenotype-altering effects after certain environmental or genetic changes.
Bendixsen, Devin P., Hayden, Eric J., Wagner, Andreas   +2 more
core   +2 more sources

Phase‐Separated Droplets Can Direct the Kinetics of Chemical Reactions Including Polymerization, Self‐Replication and Oscillating Networks

ChemSystemsChem, Volume 7, Issue 1, January 2025.
Using a numerical model, we study how phase‐separated compartments can influence chemical reactions. We show how the volume ratio (R) and partition coefficient (Kp) affect reaction rates in bimolecular reactions, and how coacervates can alter polymerization, self‐replication, and oscillating networks.
Iris B. A. Smokers, Brent S. Visser, Wojciech P. Lipiński, Karina K. Nakashima, Evan Spruijt   +4 more
wiley   +1 more source

Modulating RNA structure and catalysis: lessons from small cleaving ribozymes [PDF]

, 2009
RNA is a key molecule in life, and comprehending its structure/function relationships is a crucial step towards a more complete understanding of molecular biology.
A Berzal-Herranz, A Ferguson, A Hampel, A Hampel, A Jenne, A Ke, A Kertsburg, A Khvorova, A Nehdi, A Nehdi, A Peracchi, A Roth, A Serganov, AA Andersen, AA Rojas, AC Forster, AL Feig, AM Jose, AR Ferré-D’Amaré, AR Ferré-D’Amaré, AR Ferré-D’Amaré, AT Perrotta, AT Perrotta, AT Perrotta, B Onoa, BJ Saville, BM Chowrira, C Reymond, C Reymond, Cedric Reymond, CJ Hutchins, D Hearschlag, D Lévesque, D Lévesque, D Strohbach, DA Harris, DB McKay, DD Young, DE Draper, DH Burke, DJ Earnshaw, DJ Klein, DM Lilley, DM Lilley, DM Lilley, DY Wang, DY Wang, DY Wang, E Jankowsky, EA Alemán, EL Bertrand, EM Moody, F Nishikawa, F Nishikawa, FD Jones, G Ferbeyre, G Pljevaljcic, GA Prody, GA Robichaud, GA Soukup, GA Soukup, GA Soukup, H Bhaskaran, H Porta, HC Guo, HW Lee, HW Pley, IH Shih, J Duhamel, J Fiore, J Lipfert, J Ouellet, J Tang, J Tang, JA Nelson, JB Murray, JB Murray, JC Cochrane, JC Cochrane, JC Cochrane, JD Beaudoin, JE Barrick, JE Olive, Jean-Denis Beaudoin, Jean-Pierre Perreault, JM Buzayan, JM Buzayan, K Fiola, K Fiola, K Salehi-Ashtiani, KA Marshall, KF Blount, KH Link, L Citti, L Jaeger, LJ Bergeron, LJ Bergeron, LM Epstein, M Amarzguioui, M Araki, M Koizumi, M Kuciak, M la Peña De, M Martick, M Meli, M Sioud, M Wieland, M Zivarts, MD Been, MD Been, MD Canny, MJ Fedor, MJ Fedor, MN Win, NB Leontis, NK Vaish, P Deschênes, PB Rupert, R Russell, RA Collins, RA Collins, RH Symons, S Ananvoranich, S Cho, S Nakano, S Vauléon, S Verma, SE Butcher, SH Najafi-Shoushtari, SL Hiley, T Rastogi, TE Horton, TJ McCarthy, TJ Wilson, TK Stage, TS Wadkins, WC Winkler, WG Scott, WJ Greenleaf, Y Komatsu, Y Komatsu, Y Komatsu, Y Komatsu, Y Tanaka, YA Suh, Z Tsuchihashi, Z Zhuang, ZS Huang   +147 more
core   +2 more sources

The separation between mRNA‐ends is more variable than expected

FEBS Open Bio, Volume 14, Issue 12, Page 1985-1995, December 2024.
Both mRNA ends are in proximity despite mRNAs origin, length and secondary or tertiary structures. However, we show that the distance between native mRNA ends is not constant and varies across organisms. We found that native sequences show much larger variations than those observed in random sequences, implying the existence of a biological mechanism ...
Nancy Gerling, J. Alfredo Mendez, Eduardo Gomez, Jaime Ruiz‐Garcia   +3 more
wiley   +1 more source