Large Phenotypic Enhancement of Structured Random RNA Pools. [PDF]
, 2020 Laboratory evolution of functional RNAs has applications in many areas of chemical and synthetic biology. In vitro selections critically depend on the presence of functional molecules, such as aptamers and ribozymes, in the starting sequence pools.
Chizzolini, Fabio +5 more
core
The dynamics of the RNA world : Insights and challenges [PDF]
, 2015 The problem of the origin of life is not only one of structure but also that of dynamics. Ever since the seminal result of Manfred Eigen in 1971 showing that early template replication suffers from an error threshold, research has tackled the issue of ...
Boza, Gergely +5 more
core +1 more source
The Plant Genome, Volume 18, Issue 2, June 2025.Abstract Multiplex gene editing allows for the simultaneous targeting and mutagenesis of multiple loci in a genome. This tool is particularly valuable for plant genetic improvement, as plant genomes often require mutations at multiple loci to confer useful and/or novel traits.
Junqi Liu +14 more
wiley +1 more source
Polyethylenimine and its derivates: investigation of in vivo fate, subcellular trafficking and development of novel vector systems [PDF]
, 2003 In this dissertation several aspects of polymer based gene delivery were investigated. First, key issues in subcellular processing of electrostatic polymer/nucleic acid complexes were investigated and new insights into mechanisms involved
Merdan, Thomas, Merdan, Thomas
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Selfishness versus functional cooperation in a stochastic protocell model [PDF]
, 2010 How to design an "evolvable" artificial system capable to increase in complexity? Although Darwin's theory of evolution by natural selection obviously offers a firm foundation, little hope of success seems to be expected from the explanatory adequacy of ...
Agresti +56 more
core +2 more sources
Nanosecond Hyperquenching for Electron Cryo‐Microscopy Without Air‐Water Interface Artifacts
Chemistry – A European Journal, Volume 31, Issue 29, May 22, 2025.A novel method for the ultrafast vitrification of the biological assemblies is assayed by the electron cryotomography. Protein specimens prepared by this method inherently lack the spatial/structural artifacts, which derive from the surface tension at the air‐water interface (AWI).
Utz H. Ermel +2 more
wiley +1 more source
Correcting pervasive errors in RNA crystallography through enumerative structure prediction
, 2012 Three-dimensional RNA models fitted into crystallographic density maps exhibit pervasive conformational ambiguities, geometric errors and steric clashes. To address these problems, we present enumerative real-space refinement assisted by electron density
AT Brunger +27 more
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Amino acid-dependent stability of the acyl linkage in aminoacyl-tRNA. [PDF]
, 2014 Aminoacyl-tRNAs are the biologically active substrates for peptide bond formation in protein synthesis. The stability of the acyl linkage in each aminoacyl-tRNA, formed through an ester bond that connects the amino acid carboxyl group with the tRNA ...
, +5 more
core +2 more sources
Simple and Efficient Transformation and Gene Editing of Marchantia polymorpha Spores
Current Protocols, Volume 5, Issue 5, May 2025.Abstract Marchantia polymorpha (Marchantia) has become a model species for liverwort studies, owing to its rapid growth in vitro, ease of propagation, simple genetics, small genome, haploid‐dominant life cycle, and because it is amenable to genetic transformation.
Rebecca M. Yorker +5 more
wiley +1 more source
Dual roles of protein tyrosine phosphatase kappa in coordinating angiogenesis induced by pro-angiogenic factors [PDF]
, 2017 A potential role may be played by receptor-type protein tyrosine phosphatase kappa (PTPRK) in angiogenesis due to its critical function in coordinating intracellular signal transduction from various receptors reliant on tyrosine phosphorylation.
Chen, Gang +4 more
core +1 more source